tig (C. jejuni subsp. jejuni NCTC 11168 = ATCC 700819 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  tig
Gene Name:  trigger factor
Original Clone ID:
11188o2BCt15p1
Keyword:
full-length cds
Species:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
CjCD00336601
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1356nts
Open reading frame: 1 to 1356

GAAGTAAAGGCAAAGCAACTAGATTCTGTTAATGCGACTGCTAGTGTAAAAATTCCTTCA
GGAATGATCAAAAGTGAAGTAGAAAATTTAGCAAAAAAAGCTTCTAAAAGTGTAAAAATG
GATGGGTTTAGACCAGGAAAAGTTCCTGTAAGTGCCGTACTTAAAAGATATGAAAGAGAG
CTCACTCAAGATGCAGAACAAAATCTTTTTAAATCCGCTGTAAATAGCGCTTTACAAGAA
CTTAAAAAAGAAAATAAAGAGCTTGTAGGTGAGCCATATTTTGAAAAATTTGATCGTAAA
GATGGAGAAATTATTGCTGAATTAATACTTTCTTTTAAACCTGAAATCAAACTTGAGGGT
TATGAAAAACTCATCCCTGAATATCAAACTCCAAAAGTAAGTAAAAAAGAAATTGATGAG
AAAAAAGATGAGCTTTTAAAGCGTTTTGCTACTCCTGAAGCTATAAAAACAAAAAGAGCT
TTAAAAGAAGGTGATTTTGCAAAATTTGATTTTGAAGGTTTTGTAGATGATAAAGCTTTT
GAAGGTGGTAAAGCTGAAAATTATGTTTTAGAAATTGGTTCTAAGCAATTTATTCCAGGT
TTTGAAGATGGTATGGTGGGAATGAAAATAGGTGAAGAAAAAGATATCAAAGTAACTTTC
CCTAAAGAATACGGCGCAGCGCATTTAGCGGGTAAAGATGCAGTATTTAAAGTAAAACTA
CATGAAATTCAAGAGCTTAAAATTCCAGAACTTGATGATGAAATGCTTAAAAAACTTTTA
CCAGGAGAAGAAAAAGCTAGTGTTGAAGTTTTAGATGAAAAATTAAAAGAGCAAATCAAA
AATGAAAAACTTTTCAAACTTGTAAATGATGAATTAAAAGGCAAATTTGCAGATGCTTTG
ATTGAAAAATATAATTTTGATTTACCTAAAGGCATAGTAGAACAAGAAACTGATATGCAG
ATGCGTGCGGCTTTTAATACTTTTAGCGAAAAAGAAATTGAAGAGCTTAAAGCAAGTAAA
GAAAAATATCAAGAAAAACGTGATTCTTTTAAAGAAGAAGCACAAAAAAGTGTAAAACTT
ACTTTTATCATTGATGAACTTGCAAAATTGCGTAAGATTGAAGTAAATGATCAAGAATTG
ATTCAAGCGATTTATTTTGAAGCATATCGCTATGGTATGAATCCAAAAGAGCATTTAGAA
AACTATAAAAAACAAGGAGCTTTGCCTGCTGTGAAAATGGCTTTAATTGAAGAAAAACTT
TTTAATGATATTTTTATACCAAAAACAGAAAAATCAGAAAAAGTGAGCAAAAAAGAGAAA
GAAGATAAAGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

EVKAKQLDSVNATASVKIPSGMIKSEVENLAKKASKSVKMDGFRPGKVPVSAVLKRYERE
LTQDAEQNLFKSAVNSALQELKKENKELVGEPYFEKFDRKDGEIIAELILSFKPEIKLEG
YEKLIPEYQTPKVSKKEIDEKKDELLKRFATPEAIKTKRALKEGDFAKFDFEGFVDDKAF
EGGKAENYVLEIGSKQFIPGFEDGMVGMKIGEEKDIKVTFPKEYGAAHLAGKDAVFKVKL
HEIQELKIPELDDEMLKKLLPGEEKASVEVLDEKLKEQIKNEKLFKLVNDELKGKFADAL
IEKYNFDLPKGIVEQETDMQMRAAFNTFSEKEIEELKASKEKYQEKRDSFKEEAQKSVKL
TFIIDELAKLRKIEVNDQELIQAIYFEAYRYGMNPKEHLENYKKQGALPAVKMALIEEKL
FNDIFIPKTEKSEKVSKKEKEDKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11188o
Recommended expression in:
Not Applicable