DNASU Plasmid | GEM (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GEM

Gene Name: GTP binding protein overexpressed in skeletal muscle

Original Clone ID:

ccsbBroadEn_00629

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC022010.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00509024

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 888nts

Open reading frame: 1 to 888

Reference Sequence Alignment

ATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAG
CGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTAC
AGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCT
GACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTC
ATAGGGGAGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGAC
AGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTT
GATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAA
TGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACA
GACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAG
ACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAA
GTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACC
TCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGC
CTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAAAGGAAGGAG
AGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAAT
ATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTC

Protein sequence predicted based on nucleotide sequence

MTLNNVTMRQGTVGMQPQQQRWSIPADGRHLMVQKEPHQYSHRNRHSATPEDHCRRSWSS
DSTDSVISSESGNTYYRVVLIGEQGVGKSTLANIFAGVHDSMDSDCEVLGEDTYERTLMV
DGESATIILLDMWENKGENEWLHDHCMQVGDAYLIVYSITDRASFEKASELRIQLRRARQ
TEDIPIILVGNKSDLVRCREVSVSEGRACAVVFDCKFIETSAAVQHNVKELFEGIVRQVR
LRRDSKEKNERRLAYQKRKESMPRKARRFWGKIVAKNNKNMAFKLKSKSCHDLSVL

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GEM

Symbol Nomenclature :

GEM

SYNONYM :

KIR

Designation :

GTP-binding mitogen-induced T-cell protein|GTP-binding protein GEM|GTP-binding protein overexpressed in skeletal muscle|RAS-like protein KIR|kinase-inducible Ras-like protein

Full Nomenclature :

GTP binding protein overexpressed in skeletal muscle

GENEID :

2669

GenBank Accession :

DQ893976

HGNC :

4234

MIM :

600164

Vega :

OTTHUMG00000164392

Target GenBank:

DQ893976

Reference Sequence Alignment

  

HsCD00509024      ATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAG
NM_181702.2       ATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAG
NM_005261.3       ATGACTCTGAATAATGTCACCATGCGCCAGGGCACTGTGGGCATGCAGCCACAGCAGCAG
                  ************************************************************

HsCD00509024      CGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTAC
NM_181702.2       CGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTAC
NM_005261.3       CGCTGGAGCATCCCAGCTGATGGCAGGCATCTGATGGTCCAGAAAGAGCCCCACCAGTAC
                  ************************************************************

HsCD00509024      AGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCT
NM_181702.2       AGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCT
NM_005261.3       AGCCACCGCAACCGCCATTCTGCTACCCCTGAGGACCACTGCCGCCGAAGCTGGTCCTCT
                  ************************************************************

HsCD00509024      GACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTC
NM_181702.2       GACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTC
NM_005261.3       GACTCCACAGACTCAGTCATCTCCTCTGAGTCAGGGAACACCTACTACCGAGTGGTGCTC
                  ************************************************************

HsCD00509024      ATAGGGGAGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGAC
NM_181702.2       ATAGGGGAGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGAC
NM_005261.3       ATAGGGGAGCAGGGGGTGGGCAAGTCCACTCTGGCCAACATCTTTGCAGGTGTGCATGAC
                  ************************************************************

HsCD00509024      AGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTT
NM_181702.2       AGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTT
NM_005261.3       AGCATGGACAGCGACTGCGAGGTGCTGGGAGAAGATACATATGAACGAACCCTGATGGTT
                  ************************************************************

HsCD00509024      GATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAA
NM_181702.2       GATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAA
NM_005261.3       GATGGGGAAAGTGCAACGATTATACTCCTGGATATGTGGGAAAATAAGGGGGAAAATGAA
                  ************************************************************

HsCD00509024      TGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACA
NM_181702.2       TGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACA
NM_005261.3       TGGCTCCATGACCACTGCATGCAGGTCGGGGACGCATACCTGATTGTCTACTCAATCACA
                  ************************************************************

HsCD00509024      GACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAG
NM_181702.2       GACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAG
NM_005261.3       GACCGAGCGAGCTTCGAGAAGGCATCTGAGCTGCGAATCCAGCTCCGCAGGGCCCGGCAG
                  ************************************************************

HsCD00509024      ACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAA
NM_181702.2       ACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAA
NM_005261.3       ACAGAGGACATTCCCATAATTTTGGTTGGCAACAAAAGTGACTTAGTGCGGTGCCGAGAA
                  ************************************************************

HsCD00509024      GTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACC
NM_181702.2       GTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACC
NM_005261.3       GTGTCTGTATCAGAAGGGAGAGCCTGTGCAGTGGTGTTTGACTGCAAGTTCATCGAGACC
                  ************************************************************

HsCD00509024      TCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGC
NM_181702.2       TCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGC
NM_005261.3       TCTGCAGCTGTCCAGCACAACGTGAAGGAGCTGTTTGAGGGCATTGTGCGACAGGTGCGC
                  ************************************************************

HsCD00509024      CTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAAAGGAAGGAG
NM_181702.2       CTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAAAGGAAGGAG
NM_005261.3       CTTCGGCGGGACAGCAAGGAGAAGAATGAACGGCGGCTGGCCTACCAGAAAAGGAAGGAG
                  ************************************************************

HsCD00509024      AGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAAT
NM_181702.2       AGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAAT
NM_005261.3       AGCATGCCCAGGAAAGCCAGGCGCTTCTGGGGCAAGATCGTGGCCAAAAACAACAAGAAT
                  ************************************************************

HsCD00509024      ATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTC---
NM_181702.2       ATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTCTAG
NM_005261.3       ATGGCCTTCAAGCTCAAGTCCAAATCCTGCCATGACCTCTCTGTACTCTAG
                  ************************************************