DNASU Plasmid | KCNA10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: KCNA10

Gene Name: potassium voltage-gated channel, shaker-related subfamily, member 10

Original Clone ID:

ccsbBroadEn_06473

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC074990.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00515872

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1534nts

Open reading frame: 1 to 1534

Reference Sequence Alignment

ATGGATGTGTGTGGCTGGAAAGAAATGGAGGTTGCGCTGGTCAATTTTGATAATTCAGAT
GAAATCCAAGAAGAGCCAGGCTATGCCACAGACTTCGACTCAACCAGCCCAAAAGGCCGG
CCTGGGGGCAGCTCCTTCTCCAACGGGAAGATCCTCATCAGCGAAAGCACCAACCATGAG
ACGGCCTTCTCCAAGCTTCCGGGAGACTATGCTGACCCCCCAGGGCCTGAGCCAGTGGTC
CTAAATGAAGGAAACCAGCGGGTGATCATCAACATTGCTGGGCTGAGATTTGAGACCCAG
CTCAGAACCCTTAGTCAGTTCCCAGAGACTCTCCTGGGAGACCGGGAGAAAAGGATGCAG
TTCTTTGACTCCATGAGAAATGAGTATTTCTTTGATCGTAACCGGCCCAGTTTTGATGGA
ATCCTATATTATTACCAATCTGGTGGGAAAATTCGGCGCCCAGCCAATGTTCCCATTGAT
ATCTTTGCTGATGAAATCTCCTTCTATGAGCTGGGTAGTGAGGCCATGGACCAGTTCCGG
GAGGATGAAGGCTTCATCAAAGACCCTGAAACACTGCTACCCACCAATGACATCCACCGT
CAGTTCTGGCTCCTCTTTGAGTACCCTGAAAGTTCCAGCGCTGCCCGTGCTGTGGCCGTG
GTCTCGGTGTTGGTTGTGGTCATCTCCATCACCATCTTCTGCCTGGAGACACTGCCAGAG
TTCCGGGAGGATAGGGAGCTGAAGGTGGTCAGAGACCCCAATCTCAACATGAGCAAGACA
GTCCTCTCCCAGACCATGTTCACCGACCCTTTCTTCATGGTGGAGTCTACCTGCATCGTG
TGGTTCACCTTCGAGCTGGTGCTCCGGTTCGTGGTCTGCCCCAGCAAGACTGACTTCTTC
AGGAACATCATGAACATCATTGACATCATCTCCATTATCCCCTACTTTGCAACTCTCATC
ACAGAGCTAGTCCAGGAGACAGAGCCGAGTGCCCAACAGAACATGTCCCTGGCCATCCTG
AGGATCATCCGCCTGGTGAGGGTCTTCCGCATCTTCAAGCTCTCGCGCCACTCCAAGGGG
CTGCAGATCCTCGGGCAAACACTGAAGGCGTCCATGCGGGAGTTGGGGTTGCTCATCTTC
TTTCTCTTCATTGGAGTCATCCTCTTCTCCAGTGCAGTCTACTTTGCTGAGGTGGATGAG
CCAGAGTCCCATTTCTCTAGCATTCCTGATGGCTTCTGGTGGGCAGTGGTCACCATGACA
ACTGTAGGCTATGGGGACATGTGCCCGACCACCCCAGGGGGGAAGATTGTGGGCACTCTG
TGTGCCATTGCAGGGGTCCTCACCATTGCCCTCCCTGTGCCTGTCATTGTCTCCAACTTC
AATTACTTCTACCACCGGGAGACTGAGAATGAAGAAAAGCAGAACATCCCGGGAGAAATT
GAAAGAATCCTCAACAGTGTAGGCTCAAGAATGGGCAGCACAGACTCTCTTAATAAGACC
AATGGTGGCTGTTCCACAGAGAAGTCTAGGAAA

Protein sequence predicted based on nucleotide sequence

MDVCGWKEMEVALVNFDNSDEIQEEPGYATDFDSTSPKGRPGGSSFSNGKILISESTNHE
TAFSKLPGDYADPPGPEPVVLNEGNQRVIINIAGLRFETQLRTLSQFPETLLGDREKRMQ
FFDSMRNEYFFDRNRPSFDGILYYYQSGGKIRRPANVPIDIFADEISFYELGSEAMDQFR
EDEGFIKDPETLLPTNDIHRQFWLLFEYPESSSAARAVAVVSVLVVVISITIFCLETLPE
FREDRELKVVRDPNLNMSKTVLSQTMFTDPFFMVESTCIVWFTFELVLRFVVCPSKTDFF
RNIMNIIDIISIIPYFATLITELVQETEPSAQQNMSLAILRIIRLVRVFRIFKLSRHSKG
LQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEVDEPESHFSSIPDGFWWAVVTMT
TVGYGDMCPTTPGGKIVGTLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEKQNIPGEI
ERILNSVGSRMGSTDSLNKTNGGCSTEKSRK

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

KCNA10

Symbol Nomenclature :

KCNA10

SYNONYM :

Kcn1; Kv1.8

Designation :

cyclic GMP gated potassium channel|potassium voltage-gated channel subfamily A member 10|voltage-gated potassium channel subunit Kv1.8

Full Nomenclature :

potassium voltage-gated channel, shaker-related subfamily, member 10

GENEID :

3744

HGNC :

6219

MIM :

602420

Vega :

OTTHUMG00000022785

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00515872      ATGGATGTGTGTGGCTGGAAAGAAATGGAGGTTGCGCTGGTCAATTTTGATAATTCAGAT
NM_005549.2       ATGGATGTGTGTGGCTGGAAAGAAATGGAGGTTGCGCTGGTCAATTTTGATAATTCAGAT
                  ************************************************************

HsCD00515872      GAAATCCAAGAAGAGCCAGGCTATGCCACAGACTTCGACTCAACCAGCCCAAAAGGCCGG
NM_005549.2       GAAATCCAAGAAGAGCCAGGCTATGCCACAGACTTCGACTCAACCAGCCCAAAAGGCCGG
                  ************************************************************

HsCD00515872      CCTGGGGGCAGCTCCTTCTCCAACGGGAAGATCCTCATCAGCGAAAGCACCAACCATGAG
NM_005549.2       CCTGGGGGCAGCTCCTTCTCCAACGGGAAGATCCTCATCAGCGAAAGCACCAACCATGAG
                  ************************************************************

HsCD00515872      ACGGCCTTCTCCAAGCTTCCGGGAGACTATGCTGACCCCCCAGGGCCTGAGCCAGTGGTC
NM_005549.2       ACGGCCTTCTCCAAGCTTCCGGGAGACTATGCTGACCCCCCAGGGCCTGAGCCAGTGGTC
                  ************************************************************

HsCD00515872      CTAAATGAAGGAAACCAGCGGGTGATCATCAACATTGCTGGGCTGAGATTTGAGACCCAG
NM_005549.2       CTAAATGAAGGAAACCAGCGGGTGATCATCAACATTGCTGGGCTGAGATTTGAGACCCAG
                  ************************************************************

HsCD00515872      CTCAGAACCCTTAGTCAGTTCCCAGAGACTCTCCTGGGAGACCGGGAGAAAAGGATGCAG
NM_005549.2       CTCAGAACCCTTAGTCAGTTCCCAGAGACTCTCCTGGGAGACCGGGAAAAAAGGATGCAG
                  ***********************************************.************

HsCD00515872      TTCTTTGACTCCATGAGAAATGAGTATTTCTTTGATCGTAACCGGCCCAGTTTTGATGGA
NM_005549.2       TTCTTTGACTCCATGAGAAATGAGTATTTCTTTGATCGGAACCGGCCCAGTTTTGATGGA
                  ************************************** *********************

HsCD00515872      ATCCTATATTATTACCAATCTGGTGGGAAAATTCGGCGCCCAGCCAATGTTCCCATTGAT
NM_005549.2       ATCCTATATTATTACCAATCTGGTGGGAAAATTCGGCGCCCAGCCAATGTTCCCATTGAT
                  ************************************************************

HsCD00515872      ATCTTTGCTGATGAAATCTCCTTCTATGAGCTGGGTAGTGAGGCCATGGACCAGTTCCGG
NM_005549.2       ATCTTTGCTGATGAAATCTCCTTCTATGAGCTGGGTAGTGAGGCCATGGACCAGTTCCGG
                  ************************************************************

HsCD00515872      GAGGATGAAGGCTTCATCAAAGACCCTGAAACACTGCTACCCACCAATGACATCCACCGT
NM_005549.2       GAGGATGAAGGCTTCATCAAAGACCCTGAAACACTGCTACCCACCAATGACATCCACCGT
                  ************************************************************

HsCD00515872      CAGTTCTGGCTCCTCTTTGAGTACCCTGAAAGTTCCAGCGCTGCCCGTGCTGTGGCCGTG
NM_005549.2       CAGTTCTGGCTCCTCTTTGAGTACCCTGAAAGTTCCAGCGCTGCCCGTGCTGTGGCCGTG
                  ************************************************************

HsCD00515872      GTCTCGGTGTTGGTTGTGGTCATCTCCATCACCATCTTCTGCCTGGAGACACTGCCAGAG
NM_005549.2       GTCTCGGTGTTGGTTGTGGTCATCTCCATCACCATCTTCTGCCTGGAGACACTGCCAGAG
                  ************************************************************

HsCD00515872      TTCCGGGAGGATAGGGAGCTGAAGGTGGTCAGAGACCCCAATCTCAACATGAGCAAGACA
NM_005549.2       TTCCGGGAGGATAGGGAGCTAAAGGTGGTCAGAGACCCCAATCTCAACATGAGCAAGACA
                  ********************.***************************************

HsCD00515872      GTCCTCTCCCAGACCATGTTCACCGACCCTTTCTTCATGGTGGAGTCTACCTGCATCGTG
NM_005549.2       GTCCTCTCCCAGACCATGTTCACCGACCCTTTCTTCATGGTGGAGTCTACCTGCATCGTG
                  ************************************************************

HsCD00515872      TGGTTCACCTTCGAGCTGGTGCTCCGGTTCGTGGTCTGCCCCAGCAAGACTGACTTCTTC
NM_005549.2       TGGTTCACCTTCGAGCTGGTGCTCCGGTTCGTGGTCTGCCCCAGCAAGACTGACTTCTTC
                  ************************************************************

HsCD00515872      AGGAACATCATGAACATCATTGACATCATCTCCATTATCCCCTACTTTGCAACTCTCATC
NM_005549.2       AGGAACATCATGAACATCATTGACATCATCTCCATTATCCCCTACTTTGCAACTCTCATC
                  ************************************************************

HsCD00515872      ACAGAGCTAGTCCAGGAGACAGAGCCGAGTGCCCAACAGAACATGTCCCTGGCCATCCTG
NM_005549.2       ACAGAGCTAGTCCAGGAGACAGAGCCGAGTGCCCAACAGAACATGTCCCTGGCCATCCTG
                  ************************************************************

HsCD00515872      AGGATCATCCGCCTGGTGAGGGTCTTCCGCATCTTCAAGCTCTCGCGCCACTCCAAGGGG
NM_005549.2       AGGATCATCCGCCTGGTGAGGGTCTTCCGCATCTTCAAGCTCTCGCGCCACTCCAAGGGG
                  ************************************************************

HsCD00515872      CTGCAGATCCTCGGGCAAACACTGAAGGCGTCCATGCGGGAGTTGGGGTTGCTCATCTTC
NM_005549.2       CTGCAGATCCTCGGGCAAACACTGAAGGCGTCCATGCGGGAGTTGGGGTTGCTCATCTTC
                  ************************************************************

HsCD00515872      TTTCTCTTCATTGGAGTCATCCTCTTCTCCAGTGCAGTCTACTTTGCTGAGGTGGATGAG
NM_005549.2       TTTCTCTTCATTGGAGTCATCCTCTTCTCCAGTGCAGTCTACTTTGCTGAGGTGGATGAG
                  ************************************************************

HsCD00515872      CCAGAGTCCCATTTCTCTAGCATTCCTGATGGCTTCTGGTGGGCAGTGGTCACCATGACA
NM_005549.2       CCAGAGTCCCATTTCTCTAGCATTCCTGATGGCTTCTGGTGGGCAGTGGTCACCATGACA
                  ************************************************************

HsCD00515872      ACTGTAGGCTATGGGGACATGTGCCCGACCACCCCAGGGGGGAAGATTGTGGGCACTCTG
NM_005549.2       ACTGTAGGCTATGGGGACATGTGCCCGACCACCCCAGGGGGGAAGATTGTGGGCACTCTG
                  ************************************************************

HsCD00515872      TGTGCCATTGCAGGGGTCCTCACCATTGCCCTCCCTGTGCCTGTCATTGTCTCCAACTTC
NM_005549.2       TGTGCCATTGCAGGGGTCCTCACCATTGCCCTCCCTGTGCCTGTCATTGTCTCCAACTTC
                  ************************************************************

HsCD00515872      AATTACTTCTACCACCGGGAGACTGAGAATGAAGAAAAGCAGAACATCCCGGGAGAAATT
NM_005549.2       AATTACTTCTACCACCGGGAGACTGAGAATGAAGAAAAGCAGAACATCCCAGGAGAAATT
                  **************************************************.*********

HsCD00515872      GAAAGAATCCTCAACAGTGTAGGCTCAAGAATGGGCAGCACAGACTCTCTTAATAAGACC
NM_005549.2       GAAAGAATCCTCAACAGTGTAGGCTCAAGAATGGGCAGCACAGACTCTCTTAATAAGACC
                  ************************************************************

HsCD00515872      AATGGTGGCTGTTCCACAGAGAAGTCTAGGAAA---
NM_005549.2       AATGGTGGCTGTTCCACAGAGAAGTCTAGGAAATGA
                  *********************************