Gene:
Gene Symbol:
TRPC5
Gene Name:
transient receptor potential cation channel, subfamily C, member 5
Original Clone ID:
ccsbBroadEn_07100
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC137271.1
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00516447
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCCAACTGTACTACAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTG
CAAATTGTGAGGGCTGAGACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTG
GAGAAGGGGGACTATGCCACTGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAAT
GTTAACATCAACTGCATGGACCCCTTGGGCCGGAGTGCCCTGCTCATTGCCATTGAGAAC
GAGAACCTGGAGATCATGGAGCTACTGCTGAACCACAGCGTGTATGTGGGTGATGCATTG
CTCTATGCCATACGCAAGGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAGCTACAGGCGG
CCCAGCGGAGAGAAGCAGGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACA
CCGGACATCACTCCCATCATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTG
CTTGTCCAAAAACGGGTCACTATCCCACGGCCCCACCAGATCCGCTGCAACTGTGTGGAG
TGTGTGTCTAGTTCAGAGGTAGACAGCCTGCGCCACTCTCGCTCCCGACTGAACATCTAT
AAGGCTCTGGCAAGCCCCTCACTCATTGCCTTATCAAGTGAGGACCCCATCCTAACTGCC
TTCCGTCTGGGCTGGGAGCTCAAGGAGCTCAGCAAGGTGGAGAATGAGTTCAAGGCCGAG
TATGAGGAGCTCTCTCAGCAGTGCAAGCTCTTTGCCAAAGACCTGCTGGACCAAGCTCGG
AGCTCCAGGGAACTGGAGATCATCCTCAACCATCGAGATGACCACAGTGAAGAGCTTGAC
CCTCAGAAGTACCATGACCTGGCCAAGTTGAAGGTGGCAATCAAATACCACCAGAAAGAG
TTTGTTGCTCAGCCCAACTGCCAACAGTTGCTTGCCACCCTGTGGTATGATGGCTTCCCT
GGATGGCGGCGGAAACACTGGGTAGTCAAGCTTCTAACCTGCATGACCATTGGGTTCCTG
TTTCCCATGCTGTCTATAGCCTACCTGATCTCACCCAGGAGCAACCTTGGGCTGTTCATC
AAGAAACCCTTTATCAAGTTTATCTGCCACACAGCATCCTATTTGACCTTCCTCTTTATG
CTTCTCCTGGCTTCTCAGCACATTGTCAGGACAGACCTTCATGTACAGGGGCCTCCCCCA
ACTGTCGTGGAATGGATGATATTGCCTTGGGTTCTAGGTTTCATTTGGGGTGAGATTAAG
GAAATGTGGGATGGTGGATTTACTGAATACATCCATGACTGGTGGAACCTGATGGATTTT
GCAATGAACTCCCTCTACCTGGCAACTATTTCCCTGAAGATTGTGGCCTATGTCAAGTAT
AATGGTTCTCGTCCAAGGGAGGAATGGGAAATGTGGCACCCGACTCTGATTGCGGAAGCA
CTCTTCGCAATATCCAACATTTTAAGTTCGTTGCGTCTCATATCCCTGTTCACAGCCAAC
TCCCACTTAGGACCTCTGCAGATCTCTTTGGGACGCATGCTGCTTGATATCCTCAAATTC
CTCTTTATCTACTGCCTGGTACTACTAGCTTTTGCCAATGGACTGAACCAGCTTTACTTC
TATTATGAAACCAGAGCTATCGATGAGCCTAACAACTGCAAGGGGATCCGATGTGAGAAA
CAGAACAATGCCTTCTCCACGCTCTTTGAGACTCTTCAGTCACTCTTCTGGTCTGTATTT
GGCCTTTTAAATCTATATGTCACCAATGTGAAAGCCAGACACGAATTCACCGAGTTTGTA
GGAGCTACCATGTTTGGAACATACAATGTCATCTCCCTGGTAGTGCTGCTGAACATGCTG
ATTGCTATGATGAACAACTCCTATCAGCTTATTGCCGATCATGCTGATATCGAGTGGAAG
TTTGCAAGGACGAAGCTCTGGATGAGTTACTTTGATGAAGGTGGCACCTTGCCACCTCCT
TTCAACATCATCCCCAGTCCCAAGTCATTTCTATACCTTGGTAACTGGTTCAACAACACC
TTCTGCCCCAAAAGAGACCCTGACGGTAGACGGAGAAGGCGCAACTTGAGAAGTTTCACA
GAACGCAATGCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGGAATTTA
GTCAAAAGATATGTGGCTGCTATGATAAGAAATTCCAAAACACATGAGGGACTTACAGAA
GAAAATTTTAAGGAATTAAAGCAAGACATCTCCAGCTTTCGGTATGAAGTGCTTGACCTC
TTGGGAAATAGAAAACATCCAAGGAGCTTTTCCACTAGCAGCACTGAACTGTCTCAGAGA
GACGATAATAATGATGGCAGTGGTGGGGCTCGGGCCAAATCCAAGAGTGTCTCTTTTAAT
TTAGGCTGCAAGAAAAAGACTTGCCATGGGCCACCTCTCATCAGAACCATGCCAAGGTCC
AGTGGTGCCCAAGGAAAGTCAAAAGCTGAGTCATCAAGCAAACGCTCCTTCATGGGTCCT
TCTCTCAAGAAACTGGGTCTCCTATTCTCCAAATTTAATGGTCATATGTCTGAACCCAGT
TCAGAGCCAATGTACACAATTTCTGATGGAATTGTTCAGCAGCACTGTATGTGGCAGGAC
ATCAGATATTCTCAGATGGAGAAAGGGAAAGCAGAGGCCTGTTCTCAAAGTGAAATTAAC
CTCAGTGAGGTAGAATTAGGTGAAGTCCAGGGCGCTGCTCAGAGCAGTGAATGCCCTCTA
GCCTGTTCCAGCTCTCTTCACTGTGCATCCAGCATCTGCTCCTCAAATTCTAAACTTTTA
GACTCCTCAGAGGATGTATTTGAAACTTGGGGAGAGGCTTGTGACTTGCTCATGCACAAA
TGGGGTGATGGACAGGAAGAACAAGTTACAACTCGCCTC
Protein sequence predicted based on nucleotide sequence
MAQLYYKKVNYSPYRDRIPLQIVRAETELSAEEKAFLNAVEKGDYATVKQALQEAEIYYN VNINCMDPLGRSALLIAIENENLEIMELLLNHSVYVGDALLYAIRKEVVGAVELLLSYRR PSGEKQVPTLMMDTQFSEFTPDITPIMLAAHTNNYEIIKLLVQKRVTIPRPHQIRCNCVE CVSSSEVDSLRHSRSRLNIYKALASPSLIALSSEDPILTAFRLGWELKELSKVENEFKAE YEELSQQCKLFAKDLLDQARSSRELEIILNHRDDHSEELDPQKYHDLAKLKVAIKYHQKE FVAQPNCQQLLATLWYDGFPGWRRKHWVVKLLTCMTIGFLFPMLSIAYLISPRSNLGLFI KKPFIKFICHTASYLTFLFMLLLASQHIVRTDLHVQGPPPTVVEWMILPWVLGFIWGEIK EMWDGGFTEYIHDWWNLMDFAMNSLYLATISLKIVAYVKYNGSRPREEWEMWHPTLIAEA LFAISNILSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYF YYETRAIDEPNNCKGIRCEKQNNAFSTLFETLQSLFWSVFGLLNLYVTNVKARHEFTEFV GATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFDEGGTLPPP FNIIPSPKSFLYLGNWFNNTFCPKRDPDGRRRRRNLRSFTERNADSLIQNQHYQEVIRNL VKRYVAAMIRNSKTHEGLTEENFKELKQDISSFRYEVLDLLGNRKHPRSFSTSSTELSQR DDNNDGSGGARAKSKSVSFNLGCKKKTCHGPPLIRTMPRSSGAQGKSKAESSSKRSFMGP SLKKLGLLFSKFNGHMSEPSSEPMYTISDGIVQQHCMWQDIRYSQMEKGKAEACSQSEIN LSEVELGEVQGAAQSSECPLACSSSLHCASSICSSNSKLLDSSEDVFETWGEACDLLMHK WGDGQEEQVTTRL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
