DNASU Plasmid | sll1049 (S. sp. PCC 6803 )<br>In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: sll1049

Gene Name: hypothetical protein

Original Clone ID:

SgR79-21.3

Keyword:

None

Species:

Synechocystis sp. PCC 6803

Type:

cDNA

Vector Name:

pET21_NESG

Format:

FUSION

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Rutgers University
PSI
Northeast Structural Genomics Consortium

SsCD00540614

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1692nts

Open reading frame: 51 to 1742

GGGTGCGAATTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCTATGG
TTGTCGATTCTGTGCCCGATTCATCTACTTTTGTGGGAAAAACCATTCGTCCCGTGGCCG
CTGTTTCCATCCATGGCATTGTCTTCGATGGAGAGCGTCTCTTGGCTGTGGACAGCAAAA
ATGGTTATCTGTTGGAAATCGATCCCTACACCAACAACACTAAGTTGCTCAATGGCCAGT
ACTGGCAGGAATTCATCGGGGCCACCGGCCTGGCGATCGCCGACAGGCAGTTATGGTTTA
GCTCAGGACAGTATGTGTACACCTGTAATCTGGAGGGGCCAGACCTCAAGCCCGAGGTAT
TTGCTTGGTTTGATGACCCAGTCAATGGGGTGGCCATCTGGGAAAGCACTGTGTACATCA
CCTGCCATAAACGGGGCCACATCTGGGTCTATGGCCGGGATACCCGCAAGCTAGTGGCGA
CTTTTTACAGTCCCGGCATTGGGGTGGAAAACATTACCGTGCGGGGGGAAGAACTGTGGG
TCTGTGACAGTGCTGAACAGAGTGTCTATTGCCTCGACCGGGCCACCGGGGAAATTAACT
TTAGCCTGATTACCCCCTTTGAAAGCCCCACTGGCCTGGCTTTTTATGATGATCAAGCCG
TAGGGAAAGCGACCCTCTATGTGGCCTACGTACAACAGGAACCCTACGTCCGGGACAATC
CCAACGCCGATCCTAACCATGAGTTGATGTATCGGGAGACCACCTTCATCCATCCCCTCT
ATTTCTATAACGACCCCCACCAGTGCTGTGCCTTTTCCAACGGTTACCTGGTGGAGATGC
GTTACGTTGAGGAGCTGGCCCCCCTGGACCCCGTTGATTTAAAACAAGTGGAATGGCGCA
TGGCTCTACCGGCGGTCACCCCCCGCCAAAGAATTCGCAAAATTGAATCGGTGGGTTTAC
CCTTTAGCCGCATTGATGATGTCAACGGTCAAAAGGTGGCGGTGTTTGAGTTTGACCAGC
TTGATTTAGATAGTCGGGCCGTGTTTGGCTGGCGGGTGGAAATGGAAGTATGGGGCATTA
AATATCGCCTGAAACCCAGTGATTGTGAGCATCTTCCCGCCTTACCTGAGGGTTTTGCCG
ACCGTTACCTGGTGGATAACGACAACCTCAGCATGGAAACGGACATTATTATCCGGGCGG
CGGCCGAGGCGATCGGTCGGGAAACCAACCTCCTCCGCCAAATGTTCAATATCCGGAACT
ATGTCTACGATCGCCTTTCCTACGGCATAAAACCTCATATTGATACCCCTGACTTAGCTC
TCCGCCGGGGGGTCGGTTCCTGTGGGGAATATGTGGGAGTTTTGTTAGCCCTAGCTCGTT
TGAATGGCATTGCTTGCCGCACCGCTGGCCGCTATAAATGTCCACCCCATCCCCTCCAGC
GTAATTTGCCTATGGAGCCGGACTTCAACCATGTTTGGTTTGAATTTTATTTACCTGGCA
TTGGTTGGTTGCCCATGGAATCTAACCCCGACGACACCAATGACGGTGGCCCGTACCCCA
CCCGTTTCTTTATGGGTTTGGCTTGGTACCATGCCGAAACCGCCAAGGATGTACCCTTTG
AGCAATTATTGAGTCAGGGCATTCCCGTCAATAAGCAGGTGGTTTCCATTGGTGAGTTGG
CGATTAATCATGTGCAGTTCACCATCCTGGAGGAATTAGACCCGGCGGCGCATTTGGCCC
AT

Protein sequence predicted based on nucleotide sequence

MSMVVDSVPDSSTFVGKTIRPVAAVSIHGIVFDGERLLAVDSKNGYLLEIDPYTNNTKLL
NGQYWQEFIGATGLAIADRQLWFSSGQYVYTCNLEGPDLKPEVFAWFDDPVNGVAIWEST
VYITCHKRGHIWVYGRDTRKLVATFYSPGIGVENITVRGEELWVCDSAEQSVYCLDRATG
EINFSLITPFESPTGLAFYDDQAVGKATLYVAYVQQEPYVRDNPNADPNHELMYRETTFI
HPLYFYNDPHQCCAFSNGYLVEMRYVEELAPLDPVDLKQVEWRMALPAVTPRQRIRKIES
VGLPFSRIDDVNGQKVAVFEFDQLDLDSRAVFGWRVEMEVWGIKYRLKPSDCEHLPALPE
GFADRYLVDNDNLSMETDIIIRAAAEAIGRETNLLRQMFNIRNYVYDRLSYGIKPHIDTP
DLALRRGVGSCGEYVGVLLALARLNGIACRTAGRYKCPPHPLQRNLPMEPDFNHVWFEFY
LPGIGWLPMESNPDDTNDGGPYPTRFFMGLAWYHAETAKDVPFEQLLSQGIPVNKQVVSI
GELAINHVQFTILEELDPAAHLAH

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in SgR79

Recommended expression in:

Not Applicable

Vector Name:

pET21_NESG

Synonyms:

pET21, pET21-modified, pET21Cc, pET21_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5415

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Thomas B. Acton
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCCCCGCCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI)

157

253

bacterial origin

ori

pBR322-type bacterial origin of replication

3180

3862

gene fragment

ROP

2580

2756

primer

T7 forward

T7 forward sequencing primer

283

298

primer

T7 reverse

T7 reverse sequencing primer

promoter

T7 promoter

280

299

repressor binding site

lacO

lacO regulatory sequence

258

280

repressor protein gene

LacI

752

1768

selectable marker

AmpR

ampicillin resistance gene

3960

4619

ssDNA origin

f1 ori

f1 origin

5081

5387

tag

His

C-terminal 6xHis tag

140

157

trxn termination sequence

T7 term

T7 termination sequence

Gene

Gene Symbol :

sll1049

GENEID :

953130

Locus Tag :

sll1049

GI :

16329247

Target GenBank:

None

Protein

SMART domain :

TGc : Transglutaminase/protease-like homologues

Structural Annotation Wiki :

SgR79

UniProt :

P72653

Clone

Cloning Information :

619181

HIP Master Clone ID :

516162

Original Clone ID :

SgR79-21.3

Experimental

TargetTrack - Experimental data :

SgR79

Organism

Species Specific ID:

953130

sll1049 (S. sp. PCC 6803 )In pET21_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

sll1049 (S. sp. PCC 6803 )
In pET21_NESG (His-tagged bacterial expression vector)