Gene:
Gene Symbol:
Dhaf_0378
Gene Name:
penicillin-binding protein
Original Clone ID:
APC110494.103
Keyword:
None
Species:
Desulfitobacterium hafniense DCB-2
Type:
cDNA
Vector Name:
pMCSG73
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics
DhCD00586721
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1944nts
Open reading frame: 6 to 1949
ATGCCATCTCCACCCTGGATGTAAGCAAGCTGGAAAGCCCCCTGGCCAAGCCCACCTATA
TCTACGACCAGGCAGGCAACAAGATCTCGCAGCTCTCTTCCTCCCGTATTGAGCCCGTAA
CTCTGGAACAAGTCCCTCAGCTTGCACAGCAGGCCATTATCGCTACCGAAGATAAACGCT
TCTATGAACACCAAGGTGTGGATTTCCGCTCCATCCTGCGCGCCCTCATCCAGGACCTGA
AAACCCGGAATTTCTCCGAGGTGGGCAGCACTATCAGCCAACAGCTGGCGAAAAATCTTT
TTCTCACCTCAGACAAGACCCTCAGCCGCAAGTTGAAGGAAGCCGGCTATGCCATTAAAA
TTGAAGCCACCCTCTCCAAGGAAGAGATTCTGGAAGCCTACCTCAACCATATCTATTTTG
GGGAAGGCCGCTGGGGACTTCAGGAAGCCGCCCGTTACTATTTTGGCAAAAATGCCGAAG
AGCTGAACCTGGAGGAATCCGCCCTTCTTGCCGGCATCCTCAAAGGCCCCACCATCTACT
CCCCTCTCCAAGATAAGGAACTGGCTCTCCAGCGCCGGAACATCGTCCTCGCCATGATGG
CCGACCAGGGCTATATTACCGCCAAGGAAGCCGCTCAGGCTACTGCAGCGCCCATCGCCC
TGCGCACCAAGCCTTTAGATAATTTATCCGGCAAATACGCCCCCTACGTGGATTATGTCA
TCGAAGAAGCTATCAACCGCTATGGCTTTACAGAAGATCAGATTCTCACCCTTGGTCTGC
AAATTCATACCCAAATGGATGCCAAGGTTCAACAAGCCGCCGAAACCGTCTATAAAGATG
ACCAATTCTTCCCCCAAGGCCAGAGCGATCAAAAGGTCCAAAGCGGCATCGCCATTCTTG
ACCAGCATACCGGGGCTATCCGAGGTCTGGTGGGGTATCGGGGGGAAAGCGCTTTCCGCC
AGTTTAACCATGCCTCCCAGCTCAAGCGCCAACCCGGTTCCATCATCAAACCCCTGATGG
TCTATGGCCCCGCTTTAGAAAAAGGCTATCGCCCTGATGCCCTCCTCTATGACGGTCCTC
TGGATCTGGACGGTTATGCGCCAAAGGATTGGGACGGCCAAACCCGGGGCTGGGTGACCA
TGGAAGAGGCCATTCAGCAATCCTGGAACATCCCCGCTGTCTGGTTATTTGATCAAATCG
GCATCGACACCGGAAAAGCCTTTGTGCAAAAAGCCGGCATCCCTTTAACTGAAAAGGATG
CCCATTTAAGTCTGGCTCTGGGTGGATTTGCAGAAGGAGTCTCTCCCCTGGAGATCGCCC
AGGCCTATACCGCCTTTGCCAATCAAGGGCTCATGCACACAGCTCACGCCATCACCAAAA
TTACCACCGCCGACGGACATGTCTTAGCCCAAATGCAGCCGGAAAGCGTCCAGGTCACAG
AGCCTGATCACGCCTATACCATGACCCTTCTTCTCCAAAACGTCGTCCAGCAGGGAACCG
CCCCTAAAGCCGCTCTGGGCAGTCGTCCCGTAGCCGGAAAAACCGGTTCTGTGGAGTTGC
CGCCTACCCAAGAGTTTGCAGGTATCAGCAAGGGGCAAAAAGATGTCTGGTTTGTCGGTT
ACACGCCTGAGCTGACCGCTGCCATCTGGATGGGCTATGATCAAACGGATCGGGATCATT
ACCTCACCACCTCCGGCGGCTCGGGGCCTGCCGTCGTCTTCCATGAAGTCCTCTCTTCAG
CCCTCAAGGACACCCCTATCAAACCTTTTGAGGTTCCCGCCGGGTATGTGAAGGATTGGA
ATATCTGGCCCGAAGGCTGGGATGATCAGGATGAATACGACCAAAACAAGGATGACGATA
AAGATAAAAAGGACAAAAAGGGTAAGAAGAACAAGAAAGACCCCAAAGATAAAAACGAGT
TCTGGGACCTTTTTCAGGATCTATGGAGC
Protein sequence predicted based on nucleotide sequence
ISTLDVSKLESPLAKPTYIYDQAGNKISQLSSSRIEPVTLEQVPQLAQQAIIATEDKRFY EHQGVDFRSILRALIQDLKTRNFSEVGSTISQQLAKNLFLTSDKTLSRKLKEAGYAIKIE ATLSKEEILEAYLNHIYFGEGRWGLQEAARYYFGKNAEELNLEESALLAGILKGPTIYSP LQDKELALQRRNIVLAMMADQGYITAKEAAQATAAPIALRTKPLDNLSGKYAPYVDYVIE EAINRYGFTEDQILTLGLQIHTQMDAKVQQAAETVYKDDQFFPQGQSDQKVQSGIAILDQ HTGAIRGLVGYRGESAFRQFNHASQLKRQPGSIIKPLMVYGPALEKGYRPDALLYDGPLD LDGYAPKDWDGQTRGWVTMEEAIQQSWNIPAVWLFDQIGIDTGKAFVQKAGIPLTEKDAH LSLALGGFAEGVSPLEIAQAYTAFANQGLMHTAHAITKITTADGHVLAQMQPESVQVTEP DHAYTMTLLLQNVVQQGTAPKAALGSRPVAGKTGSVELPPTQEFAGISKGQKDVWFVGYT PELTAAIWMGYDQTDRDHYLTTSGGSGPAVVFHEVLSSALKDTPIKPFEVPAGYVKDWNI WPEGWDDQDEYDQNKDDDKDKKDKKGKKNKKDPKDKNEFWDLFQDLWS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g257t,G86V
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC110494
Recommended expression in:
Not Applicable
Vector Name:
pMCSG73
Synonyms:
''
Sequencing Primer:
Forward:
MCSG73F
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
None
Size (bp):
6227
Parent Vector:
Name:
pMCSG53
Description:
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin of replication
4220
4808
ligation independent cloning
LIC
ligation independent cloning site
138
16
promoter
T7
T7 promoter
1779
1795
protease cleavage site
TVMV
TVMV protease cleavage site
218
238
protease cleavage site
TEV
TEV protease cleavage site
155
175
repressor binding site
lac operator
lac operator
1754
1776
repressor protein gene
LacI
LacI CDS
2832
3791
selectable marker
AmpR
ampicillin resistance gene
4979
5839
tRNA
ileX
ileX tRNA gene
2378
2529
tRNA
argU
argU tRNA
1862
2218
tag
NusA
N-terminal NusA
249
1708
tag
His
N-terminal 6xHis tag
200
217
tag
strep
N-terminal StrepII tag
176
199
trxn termination sequence
T7 term
T7 terminator
26
72
