DNASU Plasmid | Dhaf_2085 (D. hafniense DCB-2 ) in pMCSG68 (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: Dhaf_2085

Gene Name: ABC transporter substrate-binding protein

Original Clone ID:

APC110489.102

Keyword:

None

Species:

Desulfitobacterium hafniense DCB-2

Type:

cDNA

Vector Name:

pMCSG68

Format:

CLOSED

Source:

Midwest Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Argonne National Laboratory
Michael Endres
PSI:Biology
Midwest Center for Structural Genomics

DhCD00590594

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 996nts

Open reading frame: 51 to 1046

ATCCGCAGTTCGAAAAGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTCGACAAGTG
GTCTATCCGGGCAGGATAACCTGCCGGGGGGCGAAGGGGTATCAGCCGGCACAGGTTCTG
AGCAGGGGGAAGTGACGTATCCCCTAAGGATTAAGCATGCCTTTGGTGTGACCGTGATTG
AAAGCAAACCGGAACGGGTTGCCACCATTTCCTGGGGGAACCAGGATATACCTTTAGCTT
TAGGTGTGATTCCAGTGGGGGTGTCCAAGGCCAATTATGGGGTGACCGACGACAGCGGTT
TGCTGCCCTGGACGCTGGAAGGGTTTAAAAAGCTGGGTGTAGAGAACCCTGTTCTGTTTA
ACGATACCGACGGCTTGGATTTTGAAGCGATCAGTGATGTCCAGCCGGATGTAATTCTGG
CGGCTTACTCAGGAATCACCCAGGAAGAATACGATTTATTGAATCAAATCGCTCCCGTTG
TTGCCTATCCCACCGTGGCCTGGCAGACTTACTGGCGTGATCAGATCGTTATGGATGCCA
CTGGAATGGGCATGGAGGCAGAAGGCGAGGAGCTGGTCTCTGAGCTGGAAAAGCTGATCA
CTAAAAAAATTGCTGCCTATCCTCAGATGACCGGCAAGAATGCGGCCTTCTTCTATTTTA
TCCCCACCGATTTAGGCAAGTTCTATGTTTATCTGCCCACGGATCCCCGAGCTGCTTATC
TGACGGATCTGGGGTTAAAGGTTCCGGAAAGTGTAGCTGAACTTGCTAAAGACTCTGCCA
GCTTTGCCATTGAACTGAGTGCGGAGAATGTGGATATCCTGAAGGACGTGGATATAATCA
TTGCCTATGGTGATGAAGCCTTGCTGAAAAGCTTGCAGGCCGATCCGCTGATGGGTACAT
TGCCTGCCATTCAGAGAGGTTCAGTGGCTATGATTGAGGATGGAACTCCCCTGGCTGCTT
CCGGTACTCCCAGTGCTCTGTCCATTCCGGCTACCATCGATGATTACTTAAGCATCATTG
GCAAAGCCGCTGACTGGGTGCGATAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

STSGLSGQDNLPGGEGVSAGTGSEQGEVTYPLRIKHAFGVTVIESKPERVATISWGNQDI
PLALGVIPVGVSKANYGVTDDSGLLPWTLEGFKKLGVENPVLFNDTDGLDFEAISDVQPD
VILAAYSGITQEEYDLLNQIAPVVAYPTVAWQTYWRDQIVMDATGMGMEAEGEELVSELE
KLITKKIAAYPQMTGKNAAFFYFIPTDLGKFYVYLPTDPRAAYLTDLGLKVPESVAELAK
DSASFAIELSAENVDILKDVDIIIAYGDEALLKSLQADPLMGTLPAIQRGSVAMIEDGTP
LAASGTPSALSIPATIDDYLSIIGKAADWVR*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in MCSG-APC110489

Recommended expression in:

Not Applicable

Vector Name:

pMCSG68

Synonyms:

Sequencing Primer:

Forward: T7short

Reverse: T7 terminator

Description:

Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis and StrepII tags; rare argU and ileX codons; ampicillin resistance in bacteria; ligation independent cloning

Comments:

BglI-XbaI fragment removed from pMCSG57 and ligated into pMCSG53 digested with the same enzymes

Size (bp):

4832

Parent Vector:

None

Properties:

bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGTCGACGGAGCTCGAATTCGGATCCGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCGGTACCCTTTTCGAACTGCGGATGAGACCACAGATCTACACCAGAAGAATGATGATGATGATGGTGCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGTGGATTCAGTCAGGCGTCCCATTATCAGTGCTTCAGGAAATGGGCGGATGGGAGTCCATAGAAATGGTTCGTAGGTATGCTCACCTTGCGCCTAATCATTTGACAGAGCATGCGAGGAAAATAGACGACATTTTTGGTGATAATGTCCCAAATATGTCCCACTCTGGAATTATGGAGGATATAAAGAAGGCGTAACTGATTGAATTGTAATGGCGCGCCCTGCAGGATTCGAACCTGCGGCCCACGACTTAGAAGGTCGTTGCTCTATCCAACTGAGCTAAGGGCGCGTTGATACCGCAATGCGGTGTAATCGCGTGAATTATACGGTCAACCCTTGCTGAGTCAATGGCTTTTGATCTCGCCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCAAAAAGGCTGACGATTTCTCGTCAGCCTTTGCTATATCTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAGGGGCCATGGTAGCGGATTATAAAGTAACTCCGTGTCGCAATCCGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTACAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pBR322 origin

pBR322 origin of replication

2825

3413

ligation independent cloning

LIC

ligation independent cloning site

204

235

promoter

T7 promoter

384

400

protease cleavage site

TEV

TEV protease cleavage site

223

243

repressor protein gene

LacI

Lac I gene

1437

2396

selectable marker

AmpR

ampicillin resistance gene

3584

4444

tRNA

ileX

ileX tRNA gene

983

1134

tRNA

argU

argU tRNA gene

467

853

tag

strep

N-terminal strepII tag

250

273

tag

His

N-terminal 6xHis tag

292

309

trxn regulatory element

lac operator

lac operator sequence

359

381

trxn termination sequence

T7 term

T7 terminator

Gene

Gene Symbol :

Dhaf_2085

GENEID :

7259054

Locus Tag :

Dhaf_2085

GI :

219668123

Target GenBank:

None

Protein

Structural Annotation Wiki :

MCSG-APC110489

Clone

Cloning Information :

630783

Original Clone ID :

APC110489.102

Experimental

TargetTrack - Experimental data :

MCSG-APC110489

Organism

Species Specific ID:

7259054