Gene:
Gene Symbol:
KCND1
Gene Name:
potassium voltage-gated channel, Shal-related subfamily, member 1
Original Clone ID:
HsCD00300497
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00617888
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1944nts
Open reading frame: 1572 to 3515
AGAGGAGGAGGAGAACTCAGGCAGCTCTGCAGAGGGGGCCAGGAGCTCGGGTGTTTGGTA
CCCCCAGCCCCACCGCTGTTGCTGCTCCTGCAGCGGGGACACAGGTGAGGACGCCCCGAG
AGAGAGGGGGAGGGGGACCACCACTAGCGCTGTCCTCATCCTTCCCTGGTCCAGCCCCCA
CGCCCTAACCCATCCTGGGATAGTCCCCAGGGGTCCCACTGGACCTTGCCCTTCCCAGGC
CTCCTTCCAGGGACCCCGCACCCTCCCCCTGCCTATGCCACCTTCTCTCCTATGCCTGGC
TGTGGTTGCTTCTCTTCCTCCTGCTGCCGCTGCTGCTACTGCCACTGCCCAGTACACACC
GCCAGCCCCTCGGGGTGTGGTTTCCCCAGACCTGCCCCCAACGGTGGGACCCAGCCTTCT
CCCGGCGTCTCCTGCCAGCTTCCTGCAAAGCCCAGGAGCCCCCCTCCCTGGAGACACACC
CAACTCCCTTTCAACACCCCACCCCCCTCTTAATCCCATTTTGGGGTTAACCCCCTTCCT
CTTATTTAGTCTTCATAGATCCCCTGGGTGCTCTGGGATTGTGCCCAACTTCCTAACATT
TGAGTCCCGGCCCTTGGAATTCCCCAGGCCCCTTTTTCCTCCCTGTAGAACTCCTGAGAG
TGTCGACCAGGCTTTGTTGGCCGTCTAGATACCCACCTCCCCCAGATAACTCTCAGCCTC
TCAAAAAATAACCCAGCCCCCTGTCTACCCCTTTAGATACCCCTTCCCCTCTCCAGCCCT
ACTAGGAGGCTCTGGCCTCTCCTTTCTTCCCCAGAACTCCTGTAGAGTATAGACACCCCC
CCGGGCTATTCTTGGCAACTCTAGGCAGTCCCAAGACACCTTTCAGCCTCTTTACAACCT
CCTGGATCCACAGCCACCCCTCAAGATGCACCAGCTCCCTTTCCAGATCCATGAGGGGGG
TCTAACCCTCTTATGCTTCCCCTAGAAGTCTTGAGGGTGTGGACAACCCTCCTCACCTGT
CTCAGCCCCTCTAGAACCCCCACTTCCAGGCACTCTCAGTCTCTTTCAAGCACCTCTGGT
CCCATTTCTACCCCTCTGGATGCCCCAGTCTCCTCTCCACCACTGCAAAGGAATTCCAGG
CTCTTCTGCCTTCCCTGAGACTCTTGAGAGTGCAGAGAATTCCCCACGTGTTTCTTGGCC
CCTCTAGACGCCCCCAGACACCTCTCAGGCACAGGCTGACTCCTTTAGAATCATCTCAGT
CTCTCTAAACCCTGCCTCAGCTCCTTCTTGGCCCCATCCCCACACCCCTTTTCTGCTCTT
CTCCATGTCCCCAAGGCCCTTCTCAGTCCCTCAGAACATTGCCCAGGCCCCTCCTAGGTT
CTGTAAATGTCCCCCAGACTCCTTCCCATCTCTTTAGTTCTTCCTCCTGGTTCCTCTTGG
CCTCTCTAGACACCCCCAGTTTCCTTGTTTGGGTGGCTCAAGGTGTCTCCAAGCCCCCAC
CATCCTGGAGACAGCCACATTCTCCTAAACGCCACCCTCACTAAGTCTCCCTGGGCTTGG
GGAGCGGCACGATGGCGGCAGGCCTGGCCACGTGGCTGCCTTTTGCTCGGGCAGCAGCAG
TGGGCTGGCTGCCCCTGGCCCAGCAACCCCTGCCCCCGGCACCGGGGGTGAAGGCATCTC
GAGGAGATGAGGTTCTGGTGGTGAACGTGAGCGGACGGCGCTTTGAGACTTGGAAGAATA
CGCTGGACCGCTACCCAGACACCTTGCTGGGCAGCTCGGAGAAGGAATTCTTCTACGATG
CTGACTCAGGCGAGTACTTCTTCGATCGCGACCCTGACATGTTCCGCCATGTGCTGAACT
TCTACCGAACGGGGCGGCTGCATTGCCCACGGCAGGAGTGCATCCAGGCCTTCGACGAAG
AGCTGGCTTTCTACGGCCTGGTTCCCGAGCTAGTCGGTGACTGCTGCCTTGAAGAGTATC
GGGACCGAAAGAAGGAGAATGCCGAGCGCCTGGCAGAGGATGAGGAGGCAGAGCAGGCCG
GGGACGGCCCAGCCCTGCCAGCAGGCAGCTCCCTGCGGCAGCGGCTCTGGCGGGCCTTCG
AGAATCCACACACGAGCACCGCAGCCCTCGTTTTCTACTATGTGACCGGCTTCTTCATCG
CCGTGTCGGTCATCGCCAATGTGGTGGAGACCATCCCATGCCGCGGCTCTGCACGCAGGT
CCTCAAGGGAGCAGCCCTGTGGCGAACGCTTCCCACAGGCCTTTTTCTGCATGGACACAG
CCTGTGTACTCATATTCACAGGTGAATACCTCCTGCGGCTGTTTGCCGCCCCCAGCCGTT
GCCGCTTCCTGCGGAGTGTCATGAGCCTCATCGACGTGGTGGCCATCCTGCCCTACTACA
TTGGGCTTTTGGTGCCCAAGAACGACGATGTCTCTGGCGCCTTTGTCACCCTGCGTGTGT
TCCGGGTGTTTCGCATCTTCAAGTTCTCCAGGCACTCACAGGGCTTGAGGATTCTGGGCT
ACACACTCAAGAGCTGTGCCTCTGAGCTGGGCTTTCTCCTCTTTTCCCTAACCATGGCCA
TCATCATCTTTGCCACTGTCATGTTTTATGCTGAGAAGGGCACAAACAAGACCAACTTTA
CAAGCATCCCTGCGGCCTTCTGGTATACCATTGTCACCATGACCACGCTTGGCTACGGAG
ACATGGTGCCCAGCACCATTGCTGGCAAGATTTTCGGGTCCATCTGCTCACTCAGTGGCG
TCTTGGTCATTGCCCTGCCTGTGCCAGTCATTGTGTCCAACTTTAGCCGCATCTACCACC
AGAACCAGCGGGCTGACAAGCGCCGAGCACAGCAGAAGGTGCGCTTGGCAAGGATCCGAT
TGGCAAAGAGTGGTACCACCAATGCCTTCCTGCAGTACAAGCAGAATGGGGGCCTTGAGG
ACAGCGGCAGTGGCGAGGAACAGGCTCTTTGTGTCAGGAACCGTTCTGCCTTTGAACAGC
AACATCACCACTTGCTGCACTGTCTAGAGAAGACAACGTGCCATGAGTTCACAGATGAGC
TCACCTTCAGTGAAGCCCTGGGAGCCGTCTCGCCGGGTGGCCGCACCAGCCGTAGCACCT
CTGTGTCTTCCCAGCCAGTGGGACCCGGAAGCCTGCTGTCTTCTTGCTGCCCTCGCAGGG
CCAAGCGCCGCGCCATCCGCCTTGCCAACTCCACTGCCTCAGTCAGCCGTGGCAGCATGC
AGGAGCTGGACATGCTGGCAGGGCTGCGCAGGAGCCATGCCCCTCAGAGCCGCTCCAGCC
TCAATGCCAAGCCCCATGACAGCCTTGACCTGAACTGCGACAGCCGGGACTTCGTGGCTG
CCATTATCAGCATCCCTACCCCTCCTGCCAACACCCCAGATGAGAGCCAACCTTCCTCCC
CTGGCGGCGGTGGCAGGGCCGGCAGCACCCTCAGGAACTCCAGCCTGGGTACCCCTTGCC
TCTTCCCCGAGACTGTCAAGATCTCATCCCTGTGAGGGGTAGGCCTGCTGATTCAGAGGG
TCCTCTTCATTTTTGGGAACTCCTTTCCAAAGCCATATTTTTGGGAGGCAGAGAGGGGCA
GGCTTGGGCACCCCTTCTGCCCCCCCCACTGAGAACTATGCAATGGAGTTTCATGAAATG
GTCCACATAGTGGGGAAGTAGCCAGGAAATGAGAAACTTCCTCCCACCCCAGACATTTTT
CCTGGTGGGAGCTGAAGCACTGGGCTTCCACAGGCCCATGGCCTCCTTGCCCTAGCACAC
TGGGACTGGCCCCACTCTCCCAGCTGGACTCCTGCATGCTCCTCCCCTTGGGCTCTCAGA
TGAAGGCAAAGCTTTGATCCGACATCTGAGCTCTAGCCTAAGAAGGAGAGTTGAGATTTC
CTCCTCCCTCTGGCTGGGATATGGAGCTTTGGAGGTTCAGAGAAGAGAACCCTCACCTCT
GATCTGGCCTCTACGAGAGGTCCTCATCTCCATCTGGCCCAACAATTCCCAGATTCTGAA
GCTTGGAATGCAAACACAGGCTTCATGGGCTGTGGCCTCTGCAGCGACCTGCCATCCCCA
GGCCTTGCCTGAGGGGTCAGGCTGCCTCTCCCAACACACACTCAGATAGCACAAATTCTA
CCATCCCCTTCCCTGGCTGCTGGAAATGGACCCCGCAACCCTGTCCTCTGCTGGGCCCCC
AGCAAACTCTAGCAATAGCAGCTGCTGCCGTGTCATTATGCAAAGCCTCTGACCAGTTTG
CTGCAGCATTTACATCTGCCCTAATCAGAGGGGCCACCTCTAACTCCTCCTCCTCCTCTC
TTCTCCTCTGGTTTGCGTCCTCCCTGGTTGGGCTGGAGTCTGGACTGGCTGAGATAAGAG
CCTGGCAACCAGCAAAAGCTGGGCTGTATTTGGAGATCATGGGCTGATTCCATGTTCTTG
GGCAACAGTCCAGAAGCATCAGGGGCTCCGGCCTGGGATGTTTCTGAACTTTGGGAGTTA
TAGGAGACAGGAGGAACTTCTCCTCCTCCTCCTCCCCTACAATTCCTTTTCACATATTCC
TTTCTTCTCCCTCTTGGGTGACCTTCCAAAACTCTGCTCTCAGGCTGAAATCTGGCATCA
TCTCAGGTTCCCTGTCCCCAGCACTGTCCCCATGGAGCTGGTGGCTGACAAAGATGTAGT
TTCCATCAGTCAATAAAACCTGAGAGGAGAGATGAAAAAAAAAAAAAAAAAGAAAAAAAA
AAAAAAAA
Protein sequence predicted based on nucleotide sequence
MAAGLATWLPFARAAAVGWLPLAQQPLPPAPGVKASRGDEVLVVNVSGRRFETWKNTLDR YPDTLLGSSEKEFFYDADSGEYFFDRDPDMFRHVLNFYRTGRLHCPRQECIQAFDEELAF YGLVPELVGDCCLEEYRDRKKENAERLAEDEEAEQAGDGPALPAGSSLRQRLWRAFENPH TSTAALVFYYVTGFFIAVSVIANVVETIPCRGSARRSSREQPCGERFPQAFFCMDTACVL IFTGEYLLRLFAAPSRCRFLRSVMSLIDVVAILPYYIGLLVPKNDDVSGAFVTLRVFRVF RIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGTNKTNFTSIP AAFWYTIVTMTTLGYGDMVPSTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQR ADKRRAQQKVRLARIRLAKSGTTNAFLQYKQNGGLEDSGSGEEQALCVRNRSAFEQQHHH LLHCLEKTTCHEFTDELTFSEALGAVSPGGRTSRSTSVSSQPVGPGSLLSSCCPRRAKRR AIRLANSTASVSRGSMQELDMLAGLRRSHAPQSRSSLNAKPHDSLDLNCDSRDFVAAIIS IPTPPANTPDESQPSSPGGGGRAGSTLRNSSLGTPCLFPETVKISSL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: bacterial Marker: chloramphenicol
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
