Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
APC104012.102
Keyword:
None
Species:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Type:
cDNA
Vector Name:
pMCSG7
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
CjCD00626234
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2952nts
Open reading frame: 1 to 2952
GTGGCAAGAATTTTGGCATTTGATATAGGAATAAGCTCTATTGGCTGGGCATTTAGTGAA
AATGATGAGTTAAAAGATTGTGGAGTTAGGATTTTTACAAAGGTTGAAAATCCCAAAACA
GGAGAATCCTTAGCCTTGCCAAGACGCCTTGCAAGAAGTGCTAGAAAACGCCTTGCTAGA
CGCAAAGCAAGGCTAAATCACTTAAAACATTTAATCGCAAATGAATTTAAGTTAAATTAC
GAAGATTATCAAAGCTTTGATGAAAGTTTAGCCAAGGCTTATAAAGGATCTTTAATTAGC
CCTTATGAGTTAAGATTTAGAGCTTTAAACGAGCTTTTAAGTAAGCAAGATTTTGCAAGA
GTGATTTTACATATTGCAAAAAGGCGTGGCTATGATGATATTAAAAATAGTGATGATAAA
GAAAAAGGTGCGATTTTAAAAGCTATCAAGCAAAATGAAGAAAAACTAGCAAATTATCAA
AGTGTGGGCGAGTATCTTTATAAAGAGTATTTTCAAAAATTCAAAGAAAATTCCAAAGAA
TTTACAAATGTTAGAAATAAAAAAGAAAGTTATGAACGCTGCATAGCGCAAAGTTTTTTA
AAAGATGAATTAAAGCTTATTTTTAAAAAACAAAGAGAATTTGGCTTTTCTTTTTCAAAA
AAATTTGAAGAAGAGGTGCTTAGTGTAGCATTTTATAAAAGGGCTTTAAAAGATTTTTCT
CATTTGGTGGGAAATTGTAGTTTTTTTACCGATGAAAAAAGAGCGCCAAAAAATTCGCCC
TTAGCTTTTATGTTTGTAGCACTTACAAGAATTATCAATCTTTTAAACAATCTTAAAAAT
ACTGAAGGAATTTTATATACAAAAGATGATTTAAACGCCCTTTTAAATGAAGTTTTAAAA
AACGGCACTCTAACTTATAAACAAACAAAAAAGCTTTTGGGCTTAAGCGATGATTATGAA
TTTAAAGGAGAAAAAGGAACTTATTTTATTGAGTTTAAAAAATACAAAGAATTTATAAAA
GCCCTAGGTGAGCATAATTTAAGCCAAGATGATTTAAATGAAATAGCAAAAGATATTACT
CTTATAAAAGATGAGATTAAACTTAAAAAGGCTTTAGCAAAATATGATTTAAATCAAAAT
CAAATTGATAGTTTAAGCAAATTGGAATTTAAAGACCATTTAAATATAAGTTTTAAAGCC
TTAAAACTTGTCACTCCTTTAATGCTTGAAGGTAAAAAATACGATGAAGCTTGCAATGAG
CTTAATTTAAAAGTAGCAATCAATGAAGATAAAAAAGACTTTTTACCCGCATTTAATGAA
ACTTATTACAAAGATGAAGTAACTAATCCTGTGGTTTTAAGGGCGATTAAAGAATATAGA
AAGGTCTTAAATGCCTTACTTAAAAAATACGGTAAAGTGCATAAAATAAACATTGAATTA
GCAAGAGAAGTAGGTAAAAATCACAGCCAAAGAGCTAAAATAGAAAAAGAGCAAAACGAA
AACTACAAGGCCAAAAAAGACGCCGAACTTGAATGTGAAAAACTAGGCTTAAAAATAAAT
AGCAAAAATATTTTAAAATTAAGATTATTTAAAGAACAAAAAGAATTTTGTGCTTATAGT
GGAGAAAAGATTAAAATTAGTGATTTGCAAGATGAAAAAATGCTTGAAATTGATCACATT
TACCCTTATTCTAGAAGTTTTGATGATTCTTATATGAATAAAGTTTTAGTTTTTACCAAG
CAAAATCAAGAAAAACTTAATCAAACCCCCTTTGAAGCCTTTGGCAATGATAGTGCAAAA
TGGCAGAAAATTGAAGTTTTAGCAAAAAATTTGCCTACTAAAAAGCAAAAAAGAATTTTA
GATAAAAATTACAAAGACAAAGAGCAAAAAAACTTTAAAGATAGAAATCTAAACGATACT
CGCTACATAGCAAGGCTTGTTTTAAACTACACTAAAGATTATTTAGATTTTTTACCTTTA
AGCGATGATGAAAATACAAAATTAAACGATACACAAAAAGGCTCAAAAGTGCATGTAGAA
GCAAAAAGCGGTATGCTTACATCGGCTTTAAGACACACTTGGGGCTTTAGCGCTAAGGAT
AGAAATAACCATCTTCATCACGCCATCGATGCAGTAATTATTGCTTATGCAAACAACTCT
ATCGTAAAGGCTTTTTCAGATTTTAAAAAAGAGCAAGAAAGCAACAGTGCAGAACTTTAT
GCAAAAAAAATTAGTGAATTAGATTATAAAAACAAAAGGAAGTTTTTTGAGCCTTTTAGT
GGTTTTAGGCAAAAGGTTTTAGATAAAATTGATGAAATTTTTGTCTCAAAACCTGAGAGA
AAAAAGCCAAGTGGAGCTTTACATGAAGAAACATTTAGAAAAGAAGAAGAATTCTATCAA
TCATACGGCGGAAAAGAAGGAGTTTTAAAAGCCCTAGAGCTTGGTAAAATCCGCAAAGTA
AATGGTAAAATAGTTAAAAATGGCGATATGTTTAGAGTGGATATTTTTAAACACAAAAAG
ACAAATAAATTTTATGCTGTGCCAATTTATACTATGGATTTTGCTTTAAAAGTATTACCA
AATAAAGCTGTAGCACGAAGTAAAAAAGGTGAGATAAAAGACTGGATTTTAATGGATGAA
AATTATGAGTTTTGCTTTTCTTTATATAAAGATAGTTTGATTTTAATTCAAACTAAAGAT
ATGCAAGAGCCAGAATTTGTTTATTATAATGCTTTTACAAGCTCTACTGTTAGTTTGATT
GTTTCAAAACACGATAATAAATTTGAAACGCTAAGCAAAAATCAAAAAATATTGTTTAAA
AATGCAAATGAAAAAGAAGTAATCGCAAAAAGCATAGGAATTCAAAATCTAAAAGTATTT
GAAAAATACATAGTTTCAGCACTTGGGGAAGTTACAAAGGCTGAGTTTAGACAAAGAGAA
GATTTTAAAAAA
Protein sequence predicted based on nucleotide sequence
VARILAFDIGISSIGWAFSENDELKDCGVRIFTKVENPKTGESLALPRRLARSARKRLAR RKARLNHLKHLIANEFKLNYEDYQSFDESLAKAYKGSLISPYELRFRALNELLSKQDFAR VILHIAKRRGYDDIKNSDDKEKGAILKAIKQNEEKLANYQSVGEYLYKEYFQKFKENSKE FTNVRNKKESYERCIAQSFLKDELKLIFKKQREFGFSFSKKFEEEVLSVAFYKRALKDFS HLVGNCSFFTDEKRAPKNSPLAFMFVALTRIINLLNNLKNTEGILYTKDDLNALLNEVLK NGTLTYKQTKKLLGLSDDYEFKGEKGTYFIEFKKYKEFIKALGEHNLSQDDLNEIAKDIT LIKDEIKLKKALAKYDLNQNQIDSLSKLEFKDHLNISFKALKLVTPLMLEGKKYDEACNE LNLKVAINEDKKDFLPAFNETYYKDEVTNPVVLRAIKEYRKVLNALLKKYGKVHKINIEL AREVGKNHSQRAKIEKEQNENYKAKKDAELECEKLGLKINSKNILKLRLFKEQKEFCAYS GEKIKISDLQDEKMLEIDHIYPYSRSFDDSYMNKVLVFTKQNQEKLNQTPFEAFGNDSAK WQKIEVLAKNLPTKKQKRILDKNYKDKEQKNFKDRNLNDTRYIARLVLNYTKDYLDFLPL SDDENTKLNDTQKGSKVHVEAKSGMLTSALRHTWGFSAKDRNNHLHHAIDAVIIAYANNS IVKAFSDFKKEQESNSAELYAKKISELDYKNKRKFFEPFSGFRQKVLDKIDEIFVSKPER KKPSGALHEETFRKEEEFYQSYGGKEGVLKALELGKIRKVNGKIVKNGDMFRVDIFKHKK TNKFYAVPIYTMDFALKVLPNKAVARSKKGEIKDWILMDENYEFCFSLYKDSLILIQTKD MQEPEFVYYNAFTSSTVSLIVSKHDNKFETLSKNQKILFKNANEKEVIAKSIGIQNLKVF EKYIVSALGEVTKAEFRQREDFKK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC104012
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
