Gene:
Gene Symbol:
POB3
Gene Name:
Pob3p
Original Clone ID:
None
Keyword:
"Pob3p, Pob3 220 - 478"
Species:
Saccharomyces cerevisiae S288c
Type:
cDNA
Vector Name:
pKB
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Christopher P. Hill
Annie Heroux
Tim Formosa
Andrew P. VanDemark
Elliot Ferris
Mary Blanksma
Christopher P. Hill
Annie Heroux
Tim Formosa
Andrew P. VanDemark
Elliot Ferris
Mary Blanksma
ScCD00671508
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 777nts
Open reading frame: 1 to 777
ATGGCAGAAGCATTCTATGAAGAACTAAAGGAAAAGGCAGACATCGGGGAAGTCGCTGGT
GATGCAATAGTTTCCTTCCAAGACGTCTTTTTTACCACGCCAAGAGGTCGTTATGATATC
GATATTTACAAGAACTCCATTAGACTCAGGGGTAAGACCTATGAATACAAATTGCAACAT
CGTCAAATACAAAGAATTGTTTCGTTACCAAAGGCAGATGATATCCATCACTTATTGGTT
TTGGCAATTGAACCTCCTTTACGTCAAGGACAGACCACCTACCCCTTTCTTGTCTTACAA
TTTCAGAAAGATGAGGAAACAGAAGTGCAATTGAATCTAGAAGATGAAGATTATGAGGAA
AATTATAAGGATAAATTGAAAAAACAATATGATGCTAAAACTCATATAGTTTTAAGTCAT
GTATTAAAAGGTCTGACTGACCGTAGAGTCATTGTTCCTGGAGAATATAAATCCAAATAT
GATCAGTGTGCAGTTTCATGTTCTTTCAAAGCAAACGAAGGTTATTTGTATCCATTAGAT
AACGCTTTCTTCTTTTTAACTAAGCCAACTTTGTACATACCATTCAGTGATGTTAGCATG
GTAAACATTTCAAGAGCAGGACAAACTTCTACGTCATCGAGGACGTTTGATTTGGAAGTG
GTACTGCGTTCAAATAGAGGTTCTACCACTTTTGCCAACATCAGTAAGGAAGAGCAGCAA
TTATTGGAACAATTCCTAAAGTCTAAAAACCTAAGGGTGAAGAATGAAGATAGATAG
Protein sequence predicted based on nucleotide sequence
MAEAFYEELKEKADIGEVAGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYKLQH RQIQRIVSLPKADDIHHLLVLAIEPPLRQGQTTYPFLVLQFQKDEETEVQLNLEDEDYEE NYKDKLKKQYDAKTHIVLSHVLKGLTDRRVIVPGEYKSKYDQCAVSCSFKANEGYLYPLD NAFFFLTKPTLYIPFSDVSMVNISRAGQTSTSSRTFDLEVVLRSNRGSTTFANISKEEQQ LLEQFLKSKNLRVKNEDR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pKB
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with N-terminal TEV-cleavable 8xHis and GST tags; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
Parent vectors described in PMID: 10610695
Size (bp):
5152
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Christopher P. Hill
Christopher P. Hill
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
protease cleavage site
TEV
TEV protease cleavage site to remove His and GST tags
0
0
selectable marker
AmpR
ampicillin resistance
0
0
tag
His
N-terminal 8xHis tag
0
0
tag
GST
N-terminal GST tag
0
0
