Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
WISP14-42
Keyword:
"Mutation:a16c; a17g; a31c; a32g; a33c; a79c; a80g; g81t; a85c; a86g; a87c; a97c; a98g; g99t; a142c; a143g; g144c; a187c; a188g; g189t, Mutation:K6R, K11R, K27R, K29R, K33R, K48R, K63R, Ubiquitin K0, Ubiquitin K0"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15b
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Devin Christensen
Wesley I. Sundquist
Devin Christensen
HsCD00671552
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 234nts
Open reading frame: 1 to 234
ATGCAGATCTTCGTCCGCACGTTAACCGGTCGCACCATAACTCTGGAAGTTGAACCATCC
GATACCATCGAAAACGTTCGTGCTCGCATTCAAGACCGTGAAGGAATTCCACCTGATCAA
CAAAGATTGATCTTTGCCGGTCGCCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAAC
ATTCAGCGTGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGTTGATGA
Protein sequence predicted based on nucleotide sequence
MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYN IQRESTLHLVLRLRGG**
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET15b
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (e.coli) expression vector with T7 promoter and N-terminal His tag; IPTG inducible; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
5708
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
5374
5390
promoter
T7
T7 promoter
5230
5246
repressor protein gene
LacI
LacI gene
3764
4843
selectable marker
AmpR
ampicillin resistance gene
209
1066
tag
His
N-terminal 6xHis tag
5329
5347
trxn termination sequence
T7 term
T7 terminator
5450
5496
