Gene:
Gene Symbol:
AMOTL1
Gene Name:
angiomotin like 1
Original Clone ID:
WISP13-177
Keyword:
"OSFP-AMOTL1 Sundquist Lab Internal ID corresponding to this clone = WISP13-177, AMOTL1"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFastBac1-OSFP
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Matthew Lalonde
Wesley I. Sundquist
Matthew Lalonde
HsCD00671565
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 2871nts
Open reading frame: 1 to 2871
ATGGCTAGCTGGAGCCACCCGCAGTTCGAGAAAGGTGGAGGTGCCCGAGGTGGATCGGGA
GGTGGATCGTGGAGCCACCCGCAGTTCGAAAAAGGATTCGATTACAAGGATGACGATGAC
AAGGGTACAAGATCTCCGCTGGAAGTCCTGTTCCAGGGCCCCATGTGGAGGGCAAAGTTG
CGCCGGGGAACTTGTGAGCCTGCGGTGAAAGGGTCCCCTTCTGCTTGTTATAGCCCCAGT
AGTCCTGTCCAGGTTCTAGAAGACTCCACCTACTTTTCCCCAGACTTTCAGCTCTATTCT
GGGAGGCATGAAACATCTGCTTTGACGGTGGAGGCAACCAGTAGCATCAGGGAAAAAGTT
GTTGAAGATCCTCTTTGTAACTTCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAA
ATGAGAGGTTCCGAGGATGCGGCAGCTGGAACAGTATTGCAGCGGCTGATCCAGGAACAA
CTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCCATTCAGCACCAGGCCACA
GGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACCTCACTCAA
GAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACACCAG
GTGGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAG
GAACTGCCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAG
CAGCAACAGCAGCAGGGGGCGGTGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAG
AAGTCCCGAACTGAGGGGAGGCCCACTGTGAACCGTGCCAACAGTGGACAGGCGCATAAG
GACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCGCTCAGCGAGAGAATCATG
CAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCCGGCTCGGGGAATGGAAAG
GGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGCTGGACCCT
CGGGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAG
ACCCAGGAGCACGGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTC
AAGCCCTACCCTGCTCCTCAGCCTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCA
CCCCCTGAGTATGGGGTAACGAGCCGCCCATGCCAACTTCCGTTCCCATCAACCATGCAG
CAGCACAGCCCCATGTCCTCCCAGACCTCTTCCGCCAGCGGGCCACTGCACTCTGTCTCC
CTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCCCCGCCTGCCGCCTCCCCC
AGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAATGGTGGAG
ATATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGACAATGCC
GACAAGCTCCACAAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTG
GTCAAGTCTACCACCAAGCGAGAATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGC
GAGATTAGAAGACTTCATGATTTCAACAGAGACCTCCGAGATCGACTAGAGACTGCTAAC
AGGCAACTATCCAGCAGGGAATACGAAGGGCATGAAGACAAAGCTGCAGAGGGGCATTAT
GCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTAGAAATGGAGTTAGCAGCA
GTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATCCTGGACCAGGCTTTGAGC
AACGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAGCAAGCATATGTT
GAGAAAGTTGAGAAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAGCGA
GAACAGATGGAGCGGAGACTGCGGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACC
CAGCAGAAACATGGAAATGGCCAGCCAGCCAACATGCCGGAATACAATGCCCCAGCCCTC
CTGGAACTTGTGCGGGAGAAGGAGGAGCGGATCCTGGCCCTGGAGGCCGACATGACAAAG
TGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTTGCCATGAATGCCGCAGCC
ACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCACGGAATGGCAGCTACGGA
GAGAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAGGTGGTGCAGGCCAACAGA
AGGTGTCAGGACATGGAATACACTATTAAAAATCTCCATGCCAAAATCATAGAGAAAGAT
GCTATGATTAAGGTCCTGCAGCAGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCC
AGCCTACGTCCTGCCCGCTCCGTTCCATCCATAGCAGCAGCTACTGGGACACACTCTCGC
CAGACCTCTCTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAGGAAGAGAAGACCTGGAAG
GGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCATGCCTCTGCCCCACTGCTG
CCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACTACGGCAGCCAGCAGTGCC
CACGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGACAAGAGTGCCGAGCTCTTC
TGGCCCAGCATGGCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACGACCCCTGCTCACAGC
CCCGTCCTGAAACACCCAGCGGCCAAAGGGACCGCAGAGAAACTGGAGAACTCTCCTGGC
CATGGGAAGTCGCCTGACCACAGAGGCCGGGTCAGCAGCTTGCTGCACAAGCCCGAGTTC
CCTGATGGAGAGATGATGGAAGTCCTCATCTAA
Protein sequence predicted based on nucleotide sequence
MASWSHPQFEKGGGARGGSGGGSWSHPQFEKGFDYKDDDDKGTRSPLEVLFQGPMWRAKL RRGTCEPAVKGSPSACYSPSSPVQVLEDSTYFSPDFQLYSGRHETSALTVEATSSIREKV VEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLAIQHQAT GSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNE ELPTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHK DEALKELKQGHVRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDP RGPPPEYPFKTKQMMSPVSKTQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQP PPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASGPLHSVSLPLPLPMALGAPQPPPAASP SQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLHKFEKELQRISEAYESL VKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDKAAEGHY ASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYV EKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPAL LELVREKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYG ESSLEAHIWQEEEEVVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSS SLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLL PPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSMASLPSRGRLSTTP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pFastBac1-OSFP
Synonyms:
WISP13-86
Sequencing Primer:
Forward:
FasBacFwd
Reverse:
FastBacRev
Description:
Baculoviral entry vector with polyhedron promoter for gateway recombination in DHB10Bac cells to produce bacmid; ampicillin resistance in bacterial; SLIC cloning into SmaI site
Comments:
Empty vector Sundquist Lab internal database number = WISP13-86
Size (bp):
4959
Parent Vector:
None
Properties:
baculovirus/insect cell expression, ligation independent cloning (LIC)
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3pUTR
2 micron ori
FastBacRev reverse sequencing primer GATCCTCTAGTACTTCTCG
4328
4346
bacterial origin
ColE
ColE1 bacterial origin
1544
2226
poly-A signal
SV40 PolyA
SV40 late polyA
4388
4579
primer
forward primer
FasBacFwd forward sequencing primer ATACCGTCCCACCATCG
4010
4026
promoter
PH
Polyhedrin promoter
3904
4032
selectable marker
AmpR
Ampicillin resistance
787
1446
selectable marker
GenR
Gentamicin resistance
3000
3533
ssDNA origin
f1
f1 origin
19
325
transposon fragment
Tn7L
Tn7L
2709
2933
