Gene:
Gene Symbol:
AMOTL2
Gene Name:
angiomotin like 2
Original Clone ID:
WISP13-176
Keyword:
"OSFP-AMOTL2 Sundquist Lab Internal ID corresponding to this clone = WISP13-176, AMOTL2"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFastBac1-OSFP
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Matthew Lalonde
Wesley I. Sundquist
Matthew Lalonde
HsCD00671567
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 2343nts
Open reading frame: 1 to 2343
ATGGCTAGCTGGAGCCACCCGCAGTTCGAGAAAGGTGGAGGTGCCCGAGGTGGATCGGGA
GGTGGATCGTGGAGCCACCCGCAGTTCGAAAAAGGATTCGATTACAAGGATGACGATGAC
AAGGGTACAAGATCTCCGCTGGAAGTCCTGTTCCAGGGCCCCATGAGGACACTGGAAGAC
TCCTCGGGGACAGTCCTGCACCGCCTCATCCAGGAGCAGCTGCGCTACGGCAACCTGACT
GAGACGCGCACGCTGCTAGCCATCCAGCAGCAGGCCCTGAGGGGTGGGGCTGGAACTGGG
GGTACAGGGAGCCCCCAGGCCTCCCTGGAGATCCTGGCCCCAGAGGACAGTCAGGTGCTG
CAGCAGGCCACCAGGCAGGAGCCCCAGGGCCAGGAGCACCAGGGCGGTGAGAACCACCTG
GCAGAGAACACCCTCTACCGGCTATGCCCACAGCCCAGCAAGGGAGAGGAGCTGCCCACC
TATGAGGAGGCCAAAGCCCACTCGCAGTACTATGCGGCCCAGCAGGCAGGGACCCGGCCA
CATGCGGGGGACCGAGATCCCCGTGGGGCCCCGGGAGGCAGTCGGAGGCAGGACGAGGCC
CTGCGGGAGCTGAGGCATGGGCACGTGCGCTCGTTGAGTGAACGGCTCCTTCAGTTGTCC
CTGGAGAGGAACGGCGCCCGGGCCCCCAGCCACATGAGCTCCTCCCACAGCTTCCCACAG
CTGGCCCGCAACCAGCAGGGCCCCCCACTGAGGGGCCCCCCTGCTGAGGGCCCAGAGTCC
CGAGGACCCCCACCTCAGTACCCTCATGTTGTACTAGCTCATGAGACCACCACTGCTGTC
ACTGACCCACGGTACCGTGCCCGCGGCAGCCCGCACTTCCAGCATGCTGAAGTCAGGATC
CTGCAGGCCCAGGTGCCTCCTGTGTTCCTCCAACAGCAGCAGCAGTACCAGTACCTGCAG
CAATCTCAGGAGCACCCCCCTCCCCCACATCCAGCTGCTCTCGGCCATGGCCCCCTGAGC
TCCCTCAGTCCACCTGCTGTGGAGGGGCCAGTGAGTGCCCAGGCCTCCTCAGCCACCTCG
GGCAGTGCCCACCTGGCCCAGATGGAGGCCGTGCTGAGGGAGAATGCCAGGCTGCAGAGA
GACAATGAGCGGCTGCAGAGGGAGCTGGAGAGCTCTGCGGAGAAGGCTGGCCGCATTGAG
AAGCTGGAAAGCGAAATCCAGCGGCTCTCTGAGGCCCATGAGAGCCTGACCAGAGCCTCC
TCCAAGCGTGAGGCCCTGGAGAAGACCATGCGGAACAAGATGGACAGTGAAATGAGGAGG
CTGCAAGACTTCAACCGGGATCTTAGAGAGAGATTGGAATCTGCAAATCGCCGCCTGGCA
AGCAAGACACAGGAGGCCCAGGCCGGCAGTCAGGACATGGTGGCCAAGCTGCTTGCTCAG
AGCTACGAACAGCAGCAGGAGCAAGAGAAGCTGGAGCGAGAGATGGCACTGCTGCGCGGC
GCCATCGAGGACCAGCGGCGGCGTGCCGAGCTGCTGGAGCAGGCTCTGGGCAATGCGCAG
GGCCGGGCAGCTCGAGCCGAAGAGGAGCTGCGCAAGAAGCAGGCCTATGTGGAGAAAGTG
GAGCGGCTGCAGCAGGCGCTCGGGCAGCTGCAGGCAGCCTGTGAGAAGCGGGAGCAGCTG
GAGCTGCGTCTGCGGACTCGCCTGGAGCAGGAACTCAAGGCCCTGCGTGCACAGCAGAGA
CAGGCAGGTGCCCCAGGTGGTAGCAGTGGCAGTGGTGGGTCTCCAGAGCTCAGCGCCCTG
CGACTGTCAGAACAACTGCGAGAGAAGGAGGAGCAGATCCTGGCGCTGGAGGCCGACATG
ACCAAGTGGGAGCAGAAGTATTTGGAGGAACGTGCCATGAGGCAGTTTGCCATGGATGCG
GCTGCCACGGCTGCTGCTCAGCGTGACACCACTCTCATCCGACATTCCCCCCAGCCCTCA
CCCAGCAGCAGCTTCAATGAGGGTCTGCTCACTGGTGGCCACAGGCATCAGGAGATGGAA
AGCAGGTTAAAGGTGCTCCATGCCCAGATCCTGGAGAAGGATGCAGTGATCAAGGTCCTT
CAGCAGCGCTCCAGGAGAGACCCTGGCAAGGCCATCCAGGGCTCCCTGCGGCCTGCCAAG
TCGGTGCCATCTGTTTTCGCGGCTGCGGCAGCAGGAACCCAGGGCTGGCAAGGGCTCTCT
TCTAGTGAGCGACAAACAGCAGACGCCCCTGCTCGGCTGACTACAGCAGACAGAGCACCC
ACAGAGGAGCCAGTGGTCACAGCTCCCCCTGCTGCCCATGCCAAACACGGGAGCAGAGAT
GGGAGCACCCAGACTGACGGCCCCCCAGACAGCACCTCCACCTGCCTGCCACCGGAGCCT
GACAGCCTTCTGGGGTGCAGCAGTAGCCAGAGAGCAGCCTCTCTGGACTCTGTAGCTACA
TCCAGAGTCCAGGACTTGTCAGACATGGTGGAGATACTGATCTGA
Protein sequence predicted based on nucleotide sequence
MASWSHPQFEKGGGARGGSGGGSWSHPQFEKGFDYKDDDDKGTRSPLEVLFQGPMRTLED SSGTVLHRLIQEQLRYGNLTETRTLLAIQQQALRGGAGTGGTGSPQASLEILAPEDSQVL QQATRQEPQGQEHQGGENHLAENTLYRLCPQPSKGEELPTYEEAKAHSQYYAAQQAGTRP HAGDRDPRGAPGGSRRQDEALRELRHGHVRSLSERLLQLSLERNGARAPSHMSSSHSFPQ LARNQQGPPLRGPPAEGPESRGPPPQYPHVVLAHETTTAVTDPRYRARGSPHFQHAEVRI LQAQVPPVFLQQQQQYQYLQQSQEHPPPPHPAALGHGPLSSLSPPAVEGPVSAQASSATS GSAHLAQMEAVLRENARLQRDNERLQRELESSAEKAGRIEKLESEIQRLSEAHESLTRAS SKREALEKTMRNKMDSEMRRLQDFNRDLRERLESANRRLASKTQEAQAGSQDMVAKLLAQ SYEQQQEQEKLEREMALLRGAIEDQRRRAELLEQALGNAQGRAARAEEELRKKQAYVEKV ERLQQALGQLQAACEKREQLELRLRTRLEQELKALRAQQRQAGAPGGSSGSGGSPELSAL RLSEQLREKEEQILALEADMTKWEQKYLEERAMRQFAMDAAATAAAQRDTTLIRHSPQPS PSSSFNEGLLTGGHRHQEMESRLKVLHAQILEKDAVIKVLQQRSRRDPGKAIQGSLRPAK SVPSVFAAAAAGTQGWQGLSSSERQTADAPARLTTADRAPTEEPVVTAPPAAHAKHGSRD G
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pFastBac1-OSFP
Synonyms:
WISP13-86
Sequencing Primer:
Forward:
FasBacFwd
Reverse:
FastBacRev
Description:
Baculoviral entry vector with polyhedron promoter for gateway recombination in DHB10Bac cells to produce bacmid; ampicillin resistance in bacterial; SLIC cloning into SmaI site
Comments:
Empty vector Sundquist Lab internal database number = WISP13-86
Size (bp):
4959
Parent Vector:
None
Properties:
baculovirus/insect cell expression, ligation independent cloning (LIC)
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
3pUTR
2 micron ori
FastBacRev reverse sequencing primer GATCCTCTAGTACTTCTCG
4328
4346
bacterial origin
ColE
ColE1 bacterial origin
1544
2226
poly-A signal
SV40 PolyA
SV40 late polyA
4388
4579
primer
forward primer
FasBacFwd forward sequencing primer ATACCGTCCCACCATCG
4010
4026
promoter
PH
Polyhedrin promoter
3904
4032
selectable marker
AmpR
Ampicillin resistance
787
1446
selectable marker
GenR
Gentamicin resistance
3000
3533
ssDNA origin
f1
f1 origin
19
325
transposon fragment
Tn7L
Tn7L
2709
2933
