Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745718
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6825nts
Open reading frame: 1 to 6825
ATGGCGGCGGAGCGGCGCTGTGGAAGTATGCGAGACTTAAGAGCTCAAGTGACTAGTGGT
CTGCTGCCATTTCCAGAAGTGACTCTTCAAGCCCTTGGAGAAGACGAAATAACATTAGAA
TCTGTGCTTCGTGGAAAGTTTGCTGCAGGGAAAAATGGACTTGCTTGCTTGGCTTGTGGT
CCACAACTTGAGGTAGTGAACTCTATAACAGGAGAGCGATTGTCTGCTTACAGATTCAGT
GGAGTCAATGAACAGCCTCCTGTAGTTTTAGCTGTGAAAGAATTCTCTTGGCAGAAGCGA
ACTGGATTATTAATAGGATTGGAAGAAACAGAAGGGAGTGTTCTCTGTCTTTATGACCTT
GGAATATCAAAAGTAGTTAAAGCAGTTGTTCTTCCTGGAAGGGTAACAGCTATTGAACCT
ATAATTAATCATGGAGGAGCCAGTGCAAGCACTCAGCATTTACATCCAAGTCTGCGATGG
CTTTTTGGAGTGGCAGCTGTGGTCACTGATGTTGGACAGATCCTGCTTGTTGACCTATGT
TTGGATGACTTGTCATGCAATCAAAATGAAGTTGAAGCATCAGATCTTGAAGTTCTAACT
GGTATCCCAGCTGAAGTACCACACATTAGAGAAAGTGTAATGAGACAAGGGCGCCATCTG
TGTTTCCAGTTAGTGAGTCCAACAGGAACAGCTGTTTCAACTCTTAGTTACATAAGCAGG
ACAAATCAGCTTGCTGTAGGTTTTTCTGATGGCTATCTAGCACTTTGGAACATGAAAAGC
ATGAAAAGAGAATATTACATACAATTGGAAAGTGGACAAGTTCCTGTATATGCTGTCACT
TTTCAAGAACCTGAGAATGATCCTCGGAATTGCTGTTACCTGTGGGCTGTTCAGTCTACA
CAAGATAGTGAAGGGGATGTTTTGAGTTTGCATCTGCTGCAGCTGGCCTTTGGTAATAGA
AAGTGTTTGGCATCAGGACAAATCTTATATGAGGGGTTAGAATACTGTGAAGAAAGGTAC
ACCCTGGACCTGACAGGTGGCATGTTCCCTTTGAGGGGACAGACGAGCAATACCAAATTG
TTGGGATGCCAGAGTATAGAGAAATTTCGATCTCATGGTGACAGGGAGGAAGGCGTGAAT
GAAGCTCTATCGCCTGACACTAGTGTTTCAGTCTTTACCTGGCAAGTGAATATATATGGA
CAGGGAAAGCCTTCTGTATATTTGGGGCTTTTTGATATAAATCGTTGGTATCATGCACAA
ATGCCAGATTCGCTAAGGTCAGGAGAATATCTACATAATTGCTCTTATTTTGCACTGTGG
TCCTTGGAGTCTGTTGTAAGTAGGACTTCTCCACATGGCATCTTGGATATATTAGTACAT
GAGAGAAGTTTAAATAGAGGAGTCCCTCCTTCATATCCACCTCCCGAGCAGTTTTTTAAT
CCAAGCACTTATAATTTTGATGCCACTTGTTTGTTAAACTCGGGAGTTGTTCATTTAACT
TGTACTGGCTTTCAGAAGGAGACTCTGACTTTTTTAAAGAAATCAGGTCCATCACTCAAT
GAACTCATTCCTGATGGTTATAATCGATGTCTTGTAGCTGGCCTTCTGTCCCCAAGATTT
GTTGATGTTCAGCCTTCCAGTTTAAGCCAAGAAGAACAGTTAGAAGCTATATTGTCAGCA
GCAATTCAGACTAGTTCCCTGGGACTTTTGACTGGTTATATCCGAAGATGGATAACAGAA
GAACAACCAAATTCTGCCACTAATTTGCGCTTTGTTCTTGAATGGACGTGGAATAAAGTG
GTTCTCACAAAAGAGGAATTTGACAGACTATGTGTGCCATTATTTGATGGTTCGTGTCAT
TTCATGGATCCACAAACCATACAGTCTATCCAGCAATGCTATTTGCTTCTTAGCAATCTT
AATATAGTCTTGAGCTGTTTTGCATCAGAAGCCCGAGAGATCACTGAGAGAGGACTGATA
GACTTAAGCAATAAGTTTGTGGTTTCCCACCTGATCTGTCAGTATGCACAAGTGGTTCTT
TGGTTCTCTCATTCTGGGCTTTTACCAGAAGGCATAGATGATTCTGTGCAGTTGTCAAGG
TTATGCTACAACTACCCTGTAATTCAGAACTACTACACCAGTCGTCGACAGAAGTTTGAG
CGTTTATCAAGAGGGAAGTGGAATCCCGATTGCTTGATGATTGATGGACTGGTTTCTCAG
TTAGGAGAGAGAATTGAGAAGTTGTGGAAACGAGATGAAGGAGGCACAGGAAAATATCCT
CCTGCTAGTCTGCATGCAGTACTTGATATGTACCTATTAGACGGCGTTACTGAAGCAGCC
AAACACTCTATTACCATTTATTTGCTACTTGATATTATGTATTCCTTTCCCAACAAAACA
GACACCCCCATTGAATCTTTCCCAACTGTATTTGCCATTTCTTGGGGCCAAGTTAAACTT
ATTCAGGGGTTTTGGTTGATAGATCATAATGACTATGAGAGTGGTTTGGATCTTTTGTTT
CATCCAGCTACTGCAAAACCTTTGTCATGGCAACATTCAAAGATTATTCAGGCATTCATG
AGTCAGGGCGAGCACAGACAAGCCCTCAGATATATTCAGACAATGAAGCCAACAGTGTCC
AGTGGTAACGATGTTATCCTTCACCTCACTGTTTTGCTTTTTAATAGGTGTATGGTTGAA
GCCTGGAATTTTTTGCGGCAACATTGCAATAGGTTGAATATAGAGGAGTTACTCAAGCAC
ATGTATGAAGTCTGTCAGGAAATGGGCTTGATGGAAGATTTACTGAAGTTACCATTTACA
GACACTGAGCAGGAATGTTTAGTGAAATTTTTGCAGTCTAGTGCCAGCGTTCAGAATCAT
GAATTCCTTTTAGTGCACCATTTGCAGCGTGCCAATTATGTGCCTGCCTTGAAGCTGAAC
CAAACTCTCAAGATTAATGTTATGAATGATCGTGATCCTCGTTTGCGGGAGAGATCACTG
GCTCGAAATTCTATATTAGACCAGTATGGAAAAATCCTTCCTAGAGTCCATCGAAAATTA
GCCATTGAACGAGCTAAGCCTTATCATCTGTCAACATCATCAGTTTTTCGATTAGTTTCT
AGACCCAAACCATTATCAGCAGTGCCAAAGCAAGTTGTAACAGGAACTGTGTTGACAAGA
TCTGTTTTCATCAACAATGTGTTGTCTAAAATTGGAGAAGTTTGGGCCAGCAAAGAACCT
ATAAATAGCACCACACCTTTCAATAGTTCTAAAATAGAAGAACCATCTCCTATAGTGTAT
TCGCTCCCAGCTCCAGAGCTGCCTGAGGCATTTTTTGGAACACCAATTTCAAAAGCATCA
CAAAAAATTTCTAGACTGCTAGATTTGGTTGTTCAGCCTGTCCCCCGGCCTTCTCAGTGT
TCGGAGTTTATTCAGCAAAGCTCCATGAAATCTCCTTTGTACCTAGTATCCCGTTCACTG
CCCTCAAGTTCGCAATTAAAAGGATCGCCTCAGGCCATCTCCAGGGCTTCCGAATTACAT
TTGCTTGAAACTCCTCTTGTAGTTAAGAAAGCTAAAAGTTTGGCCATGTCAGTTACTACT
TCTGGATTTTCTGAGTTCACTCCTCAGTCCATCCTGAGGTCTACTCTTCGATCCACACCT
TTAGCATCTCCCTCTCCATCACCTGGAAGATCTCCTCAACGACTTAAAGAAACTAGAATT
TCATTTGTGGAAGAAGATGTCCACCCAAAATGGATTCCTGGGGCTGCAGATGATAGCAAA
TTAGAAGTATTTACTACACCTAAAAAATGTGCAGTTCCAGTGGAAACTGAATGGCTGAAG
AGCAAAGATAGGACCACATCTTTTTTCCTGAACAGCCCTGAAAAGGAGCATCAAGAAATG
GATGAGGGGTCACAAAGCTTAGAGAAACTGGATGTGAGCAAAGGAAACAGCAGTGTTTCA
ATCACATCCGATGAGACTACCTTAGAGTATCAGGATGCACCGTCACCGGAAGACCTTGAA
GAGACTGTTTTCACGGCCTCCAAGCCCAAAAGCTCTTCCACTGCACTAACTACTAATGTA
ACTGAACAAACTGAAAAGGATGGAGATAAAGATGTATTTGCATCAGAAGTAACTCCTTCA
GACCTACAGAAACAAATGGGCAATTTAGAAGATGCAGAAACAAAGGATCTCTTGGTTGCA
GCAGAGGCATTTTCAGAATTGAATCACTTAAGCCCGGTTCAAGGAACTGAAGCTTCTCTT
TGTGCACCATCAGTCTATGAAGGGAAAATCTTCACCCAGAAGTCCAAGGTACCAGTGTTG
GACGAAGGATTAACATCTGTTGAAACCTACACCCCTGCAATTAGAGCAAATGACAATAAA
TCTATGGCTGATGTCCTTGGTGATGGTGGAAACTCCTCGCTCACTATCTCTGAAGGTCCT
ATTGTCTCTGAGCGCAGGCTTAACCAGGAAGTAGCGCTGAACTTAAAAGAAGATCATGAA
GTAGAAGTTGGTGTACTAAAAGAAAGTGTTGACTTACCAGAAGAAAAGCTTCCAATTTCT
GACAGCCCTCCTGATACTCAAGAAATTCATGTGATTGAACAAGAAAAGCTTGAAGCTCAA
GATTCAGGAGAAGAGGCTAGGAATCTTTCATTTAATGAGTTATATCCCTCTGGCACACTT
AAGCTGCAGTACAATTTTGATACTATTGACCAACAGTTTTGTGACTTAGCTGATAACAAA
GACACTGCTGAATGTGACATTGCTGAAGTAGATGGGGAACTTTTTGTGGCTCAAAGCAAC
TTTACCCTGATATTGGAAGGTGAAGAAGGAGAAGTTGAGCCAGGTGATTTTGCATCATCT
GATGTGTTACCTAAAGCAGCTAACACAGCAACTGAAGAAAAACTGGTATGCAGTGGGGAA
AATGATAATCATGGACAAATTGCAAATTTGCCATCCGCCGTAACTAGTGACCAAAAGTCC
CAAAAAGTAGACACTTTACCATATGTGCCTGAACCTATTAAAGTGGCAATTGCAGAAAAT
TTGCTAGATGTGATTAAAGACACCAGAAGTAAAGAGATTACTTCAGACACAATGGAACAG
TCCATTCATGAAACAATACCTTTAGTGAGCCAAAACATAATGTGTCCCACTAAATTGGTC
AAATCTGCATTTAAGACTGCTCAGGAAACAAGCACAATGACTATGAATGTCAGCCAGGTT
GATGACGTGGTTTCCTCCAAAACTCGTACGAGAGGTCAACGTATCCAAAACGTGAATGTC
AAATCAGCACAACAGGAAGCATCAGCAGATGTTGCTACTCCTAAGATGCCAGGGCAGTCA
GTCAGGAAGAAAACTAGGAAGGCAAAAGAAATTTCTGAAGCTTCTGAAAACATCTATTCT
GATGTCAGAGGACTATCTCAGAACCAGCAAATACCTCAAAATTCTGTTACGCCTAGGAGA
GGAAGGAGAAAGAAAGAAGTTAATCAGGACATACTGGAAAACACCAGTTCTGTGGAACAA
GAATTACAGATCACTACAGGCAGGGAATCAAAAAGATTAAAATCATCTCAGCTTTTGGAA
CCAGCAGTTGAAGAAACTACCAAAAAAGAAGTTAAGGTTTCATCTGTTACAAAAAGGACC
CCCAGAAGGATTAAAAGATCTGTGGAAAATCAGGAAAGTGTTGAAATCATCAATGATCTG
AAAGTTAGTACGGTAACCAGTCCTAGCAGAATGATCAGAAAATTGAGGAGTACTAATTTA
GATGCCTCTGAAAATACAGGAAATAAGCAAGATGATAAATCCAGTGACAAGCAGCTGCGT
ATTAAACATGTTAGAAGGGTCAGAGGGAGAGAAGTTAGTCCATCAGATGTGAGAGAAGAC
TCCAACCTTGAGTCATCTCAGTTGACTGTTCAAGCAGAATTTGATATGTCTGCCATACCT
AGAAAACGTGGTAGACCAAGAAAAATCAATCCATCTGAAGATGTAGGATCTAAGGCTGTT
AAGGAAGAGAGAAGCCCCAAGAAGAAAGAAGCTCCCAGCATTAGAAGGAGATCTACAAGA
AATACCCCAGCTAAAAGTGAAAATGTTGATGTTGGAAAACCAGCTTTAGGAAAATCCATT
TTGGTGCCAAACGAGGAACTTTCGATGGTGATGAGCTCTAAGAAAAAACTTACAAAAAAG
ACTGAAAGTCAAAGTCAAAAACGTTCATTGCACTCCGTATCAGAAGAACGCACAGATGAA
ATGACACACAAAGAAACAAATGAGCAGGAAGAAAGATTGCTCGCCACAGCTTCTTTCACT
AAATCATCCCGCAGCAGCAGGACTCGGTCTAGCAAGGCCATCTTGTTGCCGGACCTTTCT
GAACCAAACAATGAGCCTTTATTTTCTCCAGCGTCAGAAGTTCCAAGGAAAGCAAAAGCT
AAAAAAATAGAGGTTCCTGCACAGCTGAAAGAATTAGTTTCGGATTTATCTTCTCAGTTT
GTCATCTCACCTCCTGCTTTAAGGAGCAGACAAAAAAACACATCCAATAAGAACAAGCTT
GAAGATGAACTGAAAGATGATGCACAATCAGTAGAAACTCTGGGAAAGCCAAAAGCGAAA
CGAATCAGGACGTCAAAAACAAAACAAGCAAGCAAAAACACAGAAAAAGAAAGTGCTTGG
TCACCTCCTCCCATAGAAATTCGGCTGATTTCCCCCTTGGCTAGCCCAGCTGACGGAGTC
AAGAGCAAACCAAGAAAAACTACAGAAGTGACCGGAACAGGTCTTGGAAGGAACAGAAAG
AAACTGTCTTCCTATCCAAAGCAAATTTTACGCAGAAAAATGCTG
Protein sequence predicted based on nucleotide sequence
MAAERRCGSMRDLRAQVTSGLLPFPEVTLQALGEDEITLESVLRGKFAAGKNGLACLACG PQLEVVNSITGERLSAYRFSGVNEQPPVVLAVKEFSWQKRTGLLIGLEETEGSVLCLYDL GISKVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLVDLC LDDLSCNQNEVEASDLEVLTGIPAEVPHIRESVMRQGRHLCFQLVSPTGTAVSTLSYISR TNQLAVGFSDGYLALWNMKSMKREYYIQLESGQVPVYAVTFQEPENDPRNCCYLWAVQST QDSEGDVLSLHLLQLAFGNRKCLASGQILYEGLEYCEERYTLDLTGGMFPLRGQTSNTKL LGCQSIEKFRSHGDREEGVNEALSPDTSVSVFTWQVNIYGQGKPSVYLGLFDINRWYHAQ MPDSLRSGEYLHNCSYFALWSLESVVSRTSPHGILDILVHERSLNRGVPPSYPPPEQFFN PSTYNFDATCLLNSGVVHLTCTGFQKETLTFLKKSGPSLNELIPDGYNRCLVAGLLSPRF VDVQPSSLSQEEQLEAILSAAIQTSSLGLLTGYIRRWITEEQPNSATNLRFVLEWTWNKV VLTKEEFDRLCVPLFDGSCHFMDPQTIQSIQQCYLLLSNLNIVLSCFASEAREITERGLI DLSNKFVVSHLICQYAQVVLWFSHSGLLPEGIDDSVQLSRLCYNYPVIQNYYTSRRQKFE RLSRGKWNPDCLMIDGLVSQLGERIEKLWKRDEGGTGKYPPASLHAVLDMYLLDGVTEAA KHSITIYLLLDIMYSFPNKTDTPIESFPTVFAISWGQVKLIQGFWLIDHNDYESGLDLLF HPATAKPLSWQHSKIIQAFMSQGEHRQALRYIQTMKPTVSSGNDVILHLTVLLFNRCMVE AWNFLRQHCNRLNIEELLKHMYEVCQEMGLMEDLLKLPFTDTEQECLVKFLQSSASVQNH EFLLVHHLQRANYVPALKLNQTLKINVMNDRDPRLRERSLARNSILDQYGKILPRVHRKL AIERAKPYHLSTSSVFRLVSRPKPLSAVPKQVVTGTVLTRSVFINNVLSKIGEVWASKEP INSTTPFNSSKIEEPSPIVYSLPAPELPEAFFGTPISKASQKISRLLDLVVQPVPRPSQC SEFIQQSSMKSPLYLVSRSLPSSSQLKGSPQAISRASELHLLETPLVVKKAKSLAMSVTT SGFSEFTPQSILRSTLRSTPLASPSPSPGRSPQRLKETRISFVEEDVHPKWIPGAADDSK LEVFTTPKKCAVPVETEWLKSKDRTTSFFLNSPEKEHQEMDEGSQSLEKLDVSKGNSSVS ITSDETTLEYQDAPSPEDLEETVFTASKPKSSSTALTTNVTEQTEKDGDKDVFASEVTPS DLQKQMGNLEDAETKDLLVAAEAFSELNHLSPVQGTEASLCAPSVYEGKIFTQKSKVPVL DEGLTSVETYTPAIRANDNKSMADVLGDGGNSSLTISEGPIVSERRLNQEVALNLKEDHE VEVGVLKESVDLPEEKLPISDSPPDTQEIHVIEQEKLEAQDSGEEARNLSFNELYPSGTL KLQYNFDTIDQQFCDLADNKDTAECDIAEVDGELFVAQSNFTLILEGEEGEVEPGDFASS DVLPKAANTATEEKLVCSGENDNHGQIANLPSAVTSDQKSQKVDTLPYVPEPIKVAIAEN LLDVIKDTRSKEITSDTMEQSIHETIPLVSQNIMCPTKLVKSAFKTAQETSTMTMNVSQV DDVVSSKTRTRGQRIQNVNVKSAQQEASADVATPKMPGQSVRKKTRKAKEISEASENIYS DVRGLSQNQQIPQNSVTPRRGRRKKEVNQDILENTSSVEQELQITTGRESKRLKSSQLLE PAVEETTKKEVKVSSVTKRTPRRIKRSVENQESVEIINDLKVSTVTSPSRMIRKLRSTNL DASENTGNKQDDKSSDKQLRIKHVRRVRGREVSPSDVREDSNLESSQLTVQAEFDMSAIP RKRGRPRKINPSEDVGSKAVKEERSPKKKEAPSIRRRSTRNTPAKSENVDVGKPALGKSI LVPNEELSMVMSSKKKLTKKTESQSQKRSLHSVSEERTDEMTHKETNEQEERLLATASFT KSSRSSRTRSSKAILLPDLSEPNNEPLFSPASEVPRKAKAKKIEVPAQLKELVSDLSSQF VISPPALRSRQKNTSNKNKLEDELKDDAQSVETLGKPKAKRIRTSKTKQASKNTEKESAW SPPPIEIRLISPLASPADGVKSKPRKTTEVTGTGLGRNRKKLSSYPKQILRRKML
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
