Gene:
Gene Symbol:
SGOL2
Gene Name:
shugoshin-like 2 (S. pombe)
Original Clone ID:
FLH267083.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00082459
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGTGCCCAGTGATGGAAACTGGCTCACTTTTTACCTCAGGAATTAAGAGACATTTG
AAAGACAAAAGAATTTCAAAGACTACTAAGTTGAATGTTTCTCTTGCTTCAAAAATAAAA
ACAAAAATACTAAATAATTCTTCTATTTTCAAAATATCTTTAAAGCACAACAACAGGGCA
TTAGCTCAGGCTCTTAGTAGAGAAAAAGAGAATTCTCGAAGAATTACAACTGAAAAGATG
CTATTGCAAAAAGAAGTAGAGAAACTGAATTTTGAGAACACATTTCTTCGCCTAAAGCTA
AATAACTTGAATAAGAAGCTTATAGACATAGAAGCTCTCATGAACAATAACTTGATAACT
GCAACTGAAATGAGCAGTCTTTCTGAGTTCCATCAGAGTTCCTTTCTACTGTCAGCTAGC
AAGAAGAAACGAGTTAGTAAACAGTGCAAGTTGATGCGTCTTCCATTTGCAAGGGTTCCA
TTAACTTCAAATGATGATGAAGATGAAGATAAAGAGAAAATGCAGTGTGACAACAATATT
AAATCAAAGACATTACCTGATATTCCCTCTTCAGGATCAACAACACAACCTTTATCAACT
CAGGATAATTCGGAAGTGTTATTTCTTAAAGAAAATAATCAAAATGTATATGGTTTAGAT
GATTCAGAACATATTTCTTCTATAGTTGATGTACCTCCCAGAGAAAGCCATTCCCACTCA
GACCAAAGTTCTAAGACTTCTCTAATGAGTGAGATGAGAAACGCCCAGTCTATTGGCCGC
AGATGGGAGAAACCATCTCCTAGTAATGTGACTGAAAGGAAGAAGCGTGGGTCATCTTGG
GAATCAAATAATCTTTCTGCAGACACTCCCTGTGCAACAGTTTTAGATAAACAACACATT
TCAAGTCCAGAATTAAATTGCAATAATGAGATAAATGCTCATACTAATGAAACAAATACT
GAAATGCAAAGAAATAAACAGGATCTTCCTGGCTTATCTTCTGAGTCTGCCAGAGAACCT
AATGCAGAGTGCATGAATCAAATTGAGGATAATGATGACTTTCAATTGCAGAAAACTGTG
TATGATGCTGACATGGATTTAACTGCTAGTGAAGTCAGCAAAATTGTCGCAGTCTCAACA
GGCATTAAAAAGAAAAGTAATAAAAAAACAAATGAACATGGAATGAAAACTTTCAGAAAA
GTGAAAGATTCCAGCTCTGAAAAAAAGAGAGAAAGATCAAAGAGACAGTTTAAAAATAGT
TCAGATGTCGATATTGGGGAAAAGATTGAAAACAGGACAGAAAGATCTGATGTCCTGGAT
GGCAAAAGGGGTGCAGAAGATCCCGGTTTTATTTTCAATAATGAACAGCTGGCTCAGATG
AATGAACAGCTGGCTCAGGTGAATGAACTAAAGAAAATGACCCTTCAAACTGGCTTTGAA
CAAGGTGACAGAGAAAATGTACTGTGTAATAAAAAGGAGAAAAGAATAACAAATGAGCAA
GAGGAAACATACTCTTTATCCCAAAGTTCAGGTAAATTTCACCAGGAGAGTAAATTTGAT
AAGGGTCAGAATTCCCTAACTTGTAATAAAAGTAAAGCTTCTAGACAGACATTTGTGATT
CACAAATTAGAAAAAGATAACTTACTCCCAAACCAAAAGGATAAAGTAACCATTTATGAA
AACCTAGACGTCACAAATGAATTTCACACAGCCAATCTTTCCACCAAAGATAATGGAAAT
TTATGTGATTATGGGACCCACAATATATTGGATTTGAAAAAGTATGTCACTGATATTCAA
CCCTCAGAGCAAAATGAATCAAACATTAATAAGCTTAGAAAGAAAGTAAACCGGAAGACA
GAAATAATTTCTGGAATGAACCACATGTATGAAGATAATGATAAAGATGTGGTGCATGGC
CTAAAAAAAGGTAATTTTTTTTTCAAAACCCAAGAGGATAAAGAACCTATCTCTGAAAAC
ATAGAAGTTTCCAAAGAGCTTCAAATCCCAGCTCTTTCTACTAGAGATAATGAAAATCAA
TGTGACTATAGGACCCAGAATGTGTTGGGTTTGCAAAAGCAGATCACCAATATGTACCCC
GTTCAGCAAAATGAATCAAAAGTTAATAAGAAGCTTAGGCAGAAAGTAAATCGGAAGACA
GAAATAATTTCTGAAGTGAATCATTTAGATAATGACAAAAGTATAGAATACACAGTTAAA
AGTCACTCACTCTTTTTAACGCAAAAAGATAAGGAAATAATCCCCGGAAACCTAGAAGAC
CCAAGTGAGTTTGAAACACCTGCTCTTTCTACCAAAGATAGTGGAAACCTGTATGATTCT
GAGATTCAAAATGTTTTGGGGGTGAAACATGGCCATGATATGCAACCTGCTTGTCAAAAT
GATTCAAAAATAGGTAAGAAGCCTAGACTAAATGTATGTCAAAAGTCAGAAATAATTCCT
GAAACCAACCAAATATATGAGAATGATAACAAAGGTGTACATGACCTAGAAAAAGATAAC
TTCTTCTCTCTAACCCCAAAGGATAAAGAAACAATTTCTGAAAATCTACAAGTCACAAAT
GAATTTCAAACAGTTGATCTTCTCATCAAAGATAATGGAAATTTATGTGATTATGACACC
CAGAATATATTGGAGTTGAAAAAGTATGTTACTGATAGGAAATCTGCTGAGCAAAATGAA
TCAAAAATAAATAAGCTCAGGAATAAAGTGAATTGGAAGACAGAAATAATTTCTGAAATG
AACCAGATATATGAGGATAATGATAAAGATGCACATGTCCAAGAAAGCTATACAAAAGAT
CTTGATTTTAAAGTAAATAAATCTAAACAAAAACTTGAATGCCAAGACATTATCAATAAA
CACTATATGGAAGTCAACAGTAATGAAAAGGAAAGTTGTGATCAAATTTTAGATTCCTAC
AAAGTAGTTAAAAAACGTAAGAAAGAATCATCATGCAAGGCAAAGAACATTTTGACAAAA
GCTAAGAACAAACTTGCTTCACAGTTAACAGAATCTTCACAGACATCTATCTCCTTAGAA
TCTGATTTAAAACATATTACTAGTGAAGCAGATTCTGATCCAGGAAACCCAGTTGAACTA
TGTAAGACTCAGAAGCAAAGCACTACCACTTTGAATAAAAAAGATCTCCCTTTTGTGGAA
GAAATAAAAGAAGGAGAGTGTCAGGTTAAAAAGGTAAATAAAATGACGTCTAAGTCAAAG
AAAAGGAAGACCTCCATAGATCCTTCTCCAGAGAGCCATGAAGTAATGGAAAGAATACTT
GACAGCGTTCAGGGAAAGTCTACTGTATCTGAACAAGCTGATAAGGAAAACAATTTGGAG
AATGAGAAAATGGTCAAAAATAAGCCAGACTTTTACACAAAGGCATTTAGATCTTTGTCT
GAGATACATTCACCTAACATACAAGATTCTTCCTTTGACAGTGTTCGTGAAGGTTTAGTA
CCTTTGAGCGTTTCTTCTGGTAAAAATGTGATAATAAAAGAAAATTTTGCCTTGGAGTGC
TCCCCAGCCTTTCAAGTAAGTGATGATGAGCATGAGAAGATGAACAAGATGAAATTTAAA
GTCAACCGGAGAACCCAAAAATCAGGAATAGGTGATAGACCATTACAGGACTTGTCAAAT
ACCAGTTTTGTTTCAAATAACACTGCTGAATCTGAAAATAAGTCAGAAGATCTATCTTCA
GAACGGACAAGCAGAAGAAGAAGGTGTACTCCTTTCTATTTTAAAGAGCCAAGCCTCAGA
GACAAGATGAGAAGA
Protein sequence predicted based on nucleotide sequence
MECPVMETGSLFTSGIKRHLKDKRISKTTKLNVSLASKIKTKILNNSSIFKISLKHNNRA LAQALSREKENSRRITTEKMLLQKEVEKLNFENTFLRLKLNNLNKKLIDIEALMNNNLIT ATEMSSLSEFHQSSFLLSASKKKRVSKQCKLMRLPFARVPLTSNDDEDEDKEKMQCDNNI KSKTLPDIPSSGSTTQPLSTQDNSEVLFLKENNQNVYGLDDSEHISSIVDVPPRESHSHS DQSSKTSLMSEMRNAQSIGRRWEKPSPSNVTERKKRGSSWESNNLSADTPCATVLDKQHI SSPELNCNNEINAHTNETNTEMQRNKQDLPGLSSESAREPNAECMNQIEDNDDFQLQKTV YDADMDLTASEVSKIVAVSTGIKKKSNKKTNEHGMKTFRKVKDSSSEKKRERSKRQFKNS SDVDIGEKIENRTERSDVLDGKRGAEDPGFIFNNEQLAQMNEQLAQVNELKKMTLQTGFE QGDRENVLCNKKEKRITNEQEETYSLSQSSGKFHQESKFDKGQNSLTCNKSKASRQTFVI HKLEKDNLLPNQKDKVTIYENLDVTNEFHTANLSTKDNGNLCDYGTHNILDLKKYVTDIQ PSEQNESNINKLRKKVNRKTEIISGMNHMYEDNDKDVVHGLKKGNFFFKTQEDKEPISEN IEVSKELQIPALSTRDNENQCDYRTQNVLGLQKQITNMYPVQQNESKVNKKLRQKVNRKT EIISEVNHLDNDKSIEYTVKSHSLFLTQKDKEIIPGNLEDPSEFETPALSTKDSGNLYDS EIQNVLGVKHGHDMQPACQNDSKIGKKPRLNVCQKSEIIPETNQIYENDNKGVHDLEKDN FFSLTPKDKETISENLQVTNEFQTVDLLIKDNGNLCDYDTQNILELKKYVTDRKSAEQNE SKINKLRNKVNWKTEIISEMNQIYEDNDKDAHVQESYTKDLDFKVNKSKQKLECQDIINK HYMEVNSNEKESCDQILDSYKVVKKRKKESSCKAKNILTKAKNKLASQLTESSQTSISLE SDLKHITSEADSDPGNPVELCKTQKQSTTTLNKKDLPFVEEIKEGECQVKKVNKMTSKSK KRKTSIDPSPESHEVMERILDSVQGKSTVSEQADKENNLENEKMVKNKPDFYTKAFRSLS EIHSPNIQDSSFDSVREGLVPLSVSSGKNVIIKENFALECSPAFQVSDDEHEKMNKMKFK VNRRTQKSGIGDRPLQDLSNTSFVSNNTAESENKSEDLSSERTSRRRRCTPFYFKEPSLR DKMRR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3836
Start on reference sequence
39
Mutations:
None
Discrepancy:
a26g,D9G; t365c,I122T;
a433g,I145V; g938c,G313A;
a
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
