Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00841007
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2649nts
Open reading frame: 1 to 2649
ATGGCCTCTCCAGGGGCAGGTAGGGCGCCTCCGGAGTTACCGGAGCGGAACTGCGGGTAC
CGCGAAGTCGAGTACTGGGATCAGCGCTACCAAGGCGCAGCCGATTCTGCCCCCTACGAT
TGGTTCGGGGACTTCTCCTCCTTCCGTGCCCTCCTAGAGCCGGAGCTGCGGCCCGAGGAC
CGTATCCTTGTGCTAGGTTGCGGGAACAGTGCCCTGAGCTACGAGCTGTTCCTCGGAGGC
TTCCCTAATGTGACCAGTGTGGACTACTCATCAGTCGTGGTGGCTGCCATGCAGGCTCGC
CATGCCCATGTGCCGCAGCTGCGCTGGGAAACCATGGATGTGCGGAAGCTGGACTTCCCC
AGTGCTTCTTTTGATGTGGTGCTCGAGAAGGGCACGCTGGATGCCCTGCTGGCTGGGGAA
CGAGATCCCTGGACCGTGTCCTCTGAAGGTGTCCACACTGTGGACCAGGTGTTGAGTGAG
GTGGGATTCCAGAAGGGGACAAGACAGCTGTTAGGCTCACGCACGCAGCTGGAGCTGGTC
TTAGCAGGCGCCTCTCTACTGCTGGCTGCACTGCTTCTGGGCTGCCTTGTGGCCCTAGGG
GTCCAGTACCACAGGGACCCATCCCACAGCACCTGTCTTACAGAGGCCTGCATTCGAGTG
GCTGGAAAAATCCTGGAGTCCCTGGACCGAGGGGTGAGCCCCTGTGAGGACTTTTACCAG
TTCTCCTGTGGGGGCTGGATTCGGAGGAACCCCCTGCCCGATGGGCGTTCTCGCTGGAAC
ACCTTCAACAGCCTCTGGGACCAAAACCAGGCCATACTGAAGCACCTCCTTGAAAACACC
ACCTTCAACTCCAGCAGTGAAGCTGAGCAGAAGACACAGCGCTTCTACCTATCTTGCCTA
CAGGTGGAGCGCATTGAGGAGCTGGGAGCCCAGCCACTGAGAGATCTCATTGAGAAGATT
GGTGGTTGGAACATTACGGGGCCCTGGGACCAGGACAACTTTATGGAGGTGTTGAAGGCA
GTAGCAGGGACCTACAGGGCCACCCCATTCTTCACCGTCTACATCAGTGCCGACTCTAAG
AGTTCCAACAGCAATGTTATCCAGGTGGACCAGTCTGGGCTCTTTCTGCCCTCTCGGGAT
TACTACTTAAACAGAACTGCCAATGAGAAAGTGCTCACTGCCTATCTGGATTACATGGAG
GAACTGGGGATGCTGCTGGGTGGGCGGCCCACCTCCACGAGGGAGCAGATGCAGCAGGTC
CTGGAGTTGGAGATACAGCTGGCCAACATCACAGTGCCCCAGGACCAGCGGCGCGACGAG
GAGAAGATCTACCACAAGATGAGCATTTCGGAGCTGCAGGCTCTGGCGCCCTCCATGGAC
TGGCTTGAGTTCCTGTCTTTCTTGCTGTCACCATTGGAGTTGAGTGACTCTGAGCCTGTG
GTGGTGTATGGGATGGATTATTTGCAGCAGGTTTCAGAGCTCATCAACCGCACGGAACCA
AGCATCCTGAACAATTACCTGATCTGGAACCTGGTGCAAAAGACAACCTCAAGCCTGGAC
CGACGCTTTGAGTCTGCACAAGAGAAGCTGCTGGAGACACTCTATGGCACTAAGAAGTCC
TGTGTGCCGAGGTGGCAGACCTGCATCTCCAACACGGATGACGCCCTTGGCTTTGCTTTG
GGGTCCCTCTTCGTGAAGGCCACGTTTGACCGGCAAAGCAAAGAAATTGCAGAGGGGATG
ATCAGCGAAATCCGGACCGCATTTGAGGAGGCCCTGGGACAGCTGGTTTGGATGGATGAG
AAGACCCGCCAGGCAGCCAAGGAGAAAGCAGATGCCATCTATGATATGATTGGTTTCCCA
GACTTTATCCTGGAGCCCAAAGAGCTGGATGATGTTTATGACGGGTACGAAATTTCTGAA
GATTCTTTCTTCCAAAACATGTTGAATTTGTACAACTTCTCTGCCAAGGTTATGGCTGAC
CAGCTCCGCAAGCCTCCCAGCCGGGACCAGTGGAGCATGACCCCCCAGACAGTGAATGCC
TACTACCTTCCAACTAAGAATGAGATCGTCTTCCCCGCTGGCATCCTGCAGGCCCCCTTC
TATGCCCGCAACCACCCCAAGGCCCTGAACTTCGGTGGCATCGGTGTGGTCATGGGCCAT
GAGTTGACGCATGCCTTTGATGACCAAGGGCGCGAGTATGACAAAGAAGGGAACCTGCGG
CCCTGGTGGCAGAATGAGTCCCTGGCAGCCTTCCGGAACCACACGGCCTGCATGGAGGAA
CAGTACAATCAATACCAGGTCAATGGGGAGAGGCTCAACGGCCGCCAGACGCTGGGGGAG
AACATTGCTGACAACGGCGGGCTGAAGGCTGCCTACAATGCTTACAAAGCATGGCTGAGA
AAGCATGGGGAGGAGCAGCAACTGCCAGCCGTGGGGCTCACCAACCACCAGCTCTTCTTC
GTGGGATTTGCCCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTCACGAGGGGCTG
GTGACCGACCCCCACAGCCCTGCCCGCTTCCGCGTGCTGGGCACTCTCTCCAACTCCCGT
GACTTCCTGCGGCACTTCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTGTGT
GAGGTGTGG
Protein sequence predicted based on nucleotide sequence
MASPGAGRAPPELPERNCGYREVEYWDQRYQGAADSAPYDWFGDFSSFRALLEPELRPED RILVLGCGNSALSYELFLGGFPNVTSVDYSSVVVAAMQARHAHVPQLRWETMDVRKLDFP SASFDVVLEKGTLDALLAGERDPWTVSSEGVHTVDQVLSEVGFQKGTRQLLGSRTQLELV LAGASLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQ FSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCL QVERIEELGAQPLRDLIEKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSK SSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLGGRPTSTREQMQQV LELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPV VVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKS CVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDE KTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMAD QLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGH ELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGE NIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGL VTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
