Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00850917
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 6360nts
Open reading frame: 1 to 6360
ATGAGGAGCTTCAAAAGAGTCAACTTTGGGACTCTGCTAAGCAGCCAGAAGGAGGCTGAA
GAGTTGCTGCCCGCCTTGAAGGAGTTCCTGTCCAACCCTCCAGCTGGTTTTCCCAGCAGC
CGATCTGATGCTGAGAGGAGACAAGCTTGTGATGCCATCCTGAGGGCTTGCAACCAGCAG
CTGACTGCTAAGCTAGCTTGCCCTAGGCATCTGGGGAGCCTGCTGGAGCTGGCAGAGCTG
GCCTGTGATGGCTACTTAGTGTCTACCCCACAGCGTCCTCCCCTCTACCTGGAACGAATT
CTCTTTGTCTTACTGCGGAATGCTGCTGCACAAGGAAGCCCAGAGGCCACACTCCGCCTT
GCTCAGCCCCTCCATGCCTGCTTGGTGCAGTGCTCTCGCGAGGCTGCTCCCCAGGACTAT
GAGGCCGTGGCTCGGGGCAGCTTTTCTCTGCTTTGGAAGGGGGCAGAAGCCCTGTTGGAA
CGGCGAGCTGCATTTGCAGCTCGGCTGAAGGCCTTGAGCTTCCTAGTACTCTTGGAGGAT
GAAAGTACCCCTTGTGAGGTTCCTCACTTTGCTTCTCCAACAGCCTGTCGAGCGGTAGCT
GCCCATCAGCTATTTGATGCCAGTGGCCATGGTCTAAATGAAGCAGATGCTGATTTCCTA
GATGACCTGCTCTCCAGGCACGTGATCAGAGCCTTGGTGGGTGAGAGAGGGAGCTCTTCT
GGGCTTCTTTCTCCCCAGAGGGCCCTCTGCCTCTTGGAGCTCACCTTGGAACACTGCCGT
CGCTTTTGCTGGAGCCGCCACCATGACAAAGCCATCAGCGCAGTGGAGAAGGCTCACAGT
TACCTAAGGAACACCAATCTAGCCCCTAGCCTTCAGCTATGTCAGCTGGGGGTTAAGCTG
CTGCAGGTTGGGGAGGAAGGACCTCAGGCAGTGGCCAAGCTTCTGATCAAGGCATCAGCT
GTCCTGAGCAAGAGTATGGAGGCACCATCACCCCCACTTCGGGCATTGTATGAGAGCTGC
CAGTTCTTCCTTTCAGGCCTGGAACGAGGCACCAAGAGGCGCTATAGACTTGATGCCATT
CTGAGCCTCTTTGCTTTTCTTGGAGGGTACTGCTCTCTTCTGCAGCAGCTGCGGGATGAT
GGTGTGTATGGGGGCTCCTCCAAGCAACAGCAGTCTTTTCTTCAGATGTACTTTCAGGGA
CTTCACCTCTACACTGTGGTGGTTTATGACTTTGCCCAAGGCTGTCAGATAGTTGATTTG
GCTGACCTGACCCAACTAGTGGACAGTTGTAAATCTACCGTTGTCTGGATGCTGGAGGCC
TTAGAGGGCCTGTCGGGCCAAGAGCTGACGGACCACATGGGGATGACCGCTTCTTACACC
AGTAATTTGGCCTACAGCTTCTATAGTCACAAGCTCTATGCCGAGGCCTGTGCCATCTCT
GAGCCGCTCTGTCAGCACCTGGGTTTGGTGAAGCCAGGCACTTATCCCGAGGTGCCTCCT
GAGAAGTTGCACAGGTGCTTCCGGCTACAAGTAGAGAGTTTGAAGAAACTGGGTAAACAG
GCCCAGGGCTGCAAGATGGTGATTTTGTGGCTGGCAGCCCTGCAACCCTGTAGCCCTGAA
CACATGGCTGAGCCAGTCACTTTCTGGGTTCGGGTCAAGATGGATGCGGCCAGGGCTGGA
GACAAGGAGCTACAGCTAAAGACTCTGCGAGACAGCCTCAGTGGCTGGGACCCGGAGACC
CTGGCCCTCCTGCTGAGGGAGGAGCTGCAGGCCTACAAGGCGGTGCGGGCCGACACTGGA
CAGGAACGCTTCAACATCATCTGTGACCTCCTGGAGCTGAGCCCCGAGGAGACACCAGCC
GGGGCCTGGGCACGAGCCACCCACCTGGTAGAACTGGCTCAGGTGCTCTGCTACCACGAC
TTTACGCAGCAGACCAACTGCTCTGCTCTGGATGCTATCCGGGAAGCCCTGCAGCTTCTG
GACTCTGTGAGGCCTGAGGCCCAGGCCAGAGATCAGCTTCTGGACGATAAAGCACAGGCC
TTGCTGTGGCTTTACATCTGTACTCTGGAAGCCAAAATGCAGGAAGGTATCGAGCGGGAT
CGGAGAGCCCAGGCCCCTGGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAA
GATAAACTCCAGGAAGATCGTTTCCTATACAGTAACATTGCCTTCAACCTGGCTGCAGAT
GCTGCTCAGTCCAAATGCCTGGACCAAGCCCTGGCCCTGTGGAAGGAGCTGCTTACAAAG
GGGCAGGCCCCAGCTGTACGGTGTCTCCAGCAGACAGCAGCCTCACTGCAGATCCTAGCA
GCCCTCTACCAGCTGGTGGCAAAGCCCATGCAGGCTCTGGAGGTCCTCCTGCTGCTACGG
ATTGTCTCTGAGAGACTGAAGGACCACTCGAAGGCAGCTGGCTCCTCCTGCCACATCACC
CAGCTCCTCCTGACCCTCGGCTGTCCCAGCTATGCCCAGTTACACCTGGAAGAGGCAGCA
TCGAGCCTGAAGCATCTCGATCAGACTACTGACACATACCTGCTCCTTTCCCTGACCTGT
GATCTGCTTCGAAGTCAACTCTACTGGACTCACCAGAAGGTGACCAAGGGTGTCTCTCTG
CTGCTGTCTGTGCTTCGGGATCCTGCCCTCCAGAAGTCCTCCAAGGCTTGGTACTTGCTG
CGTGTCCAGGTCCTGCAGCTGGTGGCAGCTTACCTTAGCCTCCCGTCAAACAACCTCTCA
CACTCCCTGTGGGAGCAGCTCTGTGCCCAAGGCTGGCAGACACCTGAGATAGCTCTCATA
GACTCCCATAAGCTCCTCCGAAGCATCATCCTCCTGCTGATGGGCAGTGACATTCTCTCA
ACTCAGAAAGCAGCTGTGGAGACATCGTTTTTGGACTATGGTGAAAATCTGGTACAAAAA
TGGCAGGTTCTTTCAGAGGTGCTGAGCTGCTCAGAGAAGCTGGTCTGCCACCTGGGCCGC
CTGGGTAGTGTGAGTGAAGCCAAGGCCTTTTGCTTGGAGGCCCTAAAACTTACAACAAAG
CTGCAGATACCACGCCAGTGTGCCCTGTTCCTGGTGCTGAAGGGCGAGCTGGAGCTGGCC
CGCAATGACATTGATCTCTGTCAGTCGGACCTGCAGCAGGTTCTGTTCTTGCTTGAGTCT
TGCACAGAGTTTGGTGGGGTGACTCAGCACCTGGACTCTGTGAAGAAGGTCCACCTGCAG
AAGGGGAAGCAGCAGGCCCAGGTCCCCTGTCCTCCACAGCTCCCAGAGGAGGAGCTCTTC
CTAAGAGGCCCTGCTCTAGAGCTGGTGGCCACTGTGGCCAAGGAGCCTGGCCCCATAGCA
CCTTCTACAAACTCCTCCCCAGTCTTGAAAACCAAGCCCCAGCCCATACCCAACTTCCTG
TCCCATTCACCCACCTGTGACTGCTCGCTCTGCGCCAGCCCTGTCCTCACAGCAGTCTGT
CTGCGCTGGGTATTGGTCACGGCAGGGGTGAGGCTGGCCATGGGCCACCAAGCCCAGGGT
CTGGATCTGCTGCAGGTCGTGCTGAAGGGCTGTCCTGAAGCCGCTGAGCGCCTCACCCAA
GCTCTCCAAGCTTCCCTGAATCATAAAACACCCCCCTCCTTGGTTCCAAGCCTCTTGGAT
GAGATCTTGGCTCAAGCATACACACTGTTGGCACTGGAGGGCCTGAACCAGCCATCAAAC
GAGAGCCTGCAGAAGGTTCTACAGTCAGGGCTGAAGTTTGTAGCAGCACGGATACCCCAC
CTAGAGCCCTGGCGAGCCAGCCTGCTCTTGATTTGGGCCCTCACAAAACTAGGTGGCCTC
AGCTGCTGTACTACCCAACTTTTTGCAAGCTCCTGGGGCTGGCAGCCACCATTAATAAAA
AGTGTCCCTGGCTCAGAGCCCTCTAAGACTCAGGGCCAAAAACGTTCTGGACGAGGGCGC
CAAAAGTTAGCCTCTGCTCCCCTGCGCCTCAATAATACCTCTCAGAAAGGTCTGGAAGGT
AGAGGACTGCCCTGCACACCTAAACCCCCAGACCGGATCAGGCAAGCTGGCCCTCATGTC
CCCTTCACGGTGTTTGAGGAAGTCTGCCCTACAGAGAGCAAGCCTGAAGTACCCCAGGCC
CCCAGGGTACAACAGAGAGTCCAGACGCGCCTCAAGGTGAACTTCAGTGATGACAGTGAC
TTGGAAGACCCTGTCTCAGCTGAGGCCTGGCTGGCAGAGGAGCCTAAGAGACGGGGCACT
GCTTCCCGGGGCCGGGGGCGAGCAAGGAAGGGCCTGAGCCTAAAGACGGATGCCGTGGTT
GCCCCAGGTAGTGCCCCTGGGAACCCTGGCCTGAATGGCAGGAGCCGGAGGGCCAAGAAG
GTGGCATCAAGACATTGTGAGGAGCGGCGTCCCCAGAGGGCCAGTGACCAGGCCAGGCCT
GGCCCTGAGATCATGAGGACCATCCCTGAGGAAGAACTGACTGACAACTGGAGAAAAATG
AGCTTTGAGATCCTCAGGGGCTCTGACGGGGAAGACTCAGCCTCAGGTGGGAAGACTCCA
GCTCCGGGCCCTGAGGCAGCTTCTGGAGAATGGGAGCTGCTGAGGCTGGATTCCAGCAAG
AAGAAGCTGCCCAGCCCATGCCCAGACAAGGAGAGTGACAAGGACCTTGGTCCTCGGCTC
CGGCTCCCCTCAGCCCCCGTAGCCACTGGTCTTTCTACCCTGGACTCCATCTGTGACTCC
CTGAGTGTTGCTTTCCGGGGCATTAGTCACTGTCCTCCTAGTGGGCTCTATGCCCACCTC
TGCCGCTTCCTGGCCTTGTGCCTGGGCCACCGGGATCCTTATGCCACTGCTTTCCTTGTC
ACCGAGTCTGTCTCCATCACCTGTCGCCACCAGCTGCTCACCCACCTCCACAGACAGCTC
AGCAAGGCCCAGAAGCACCGAGGATCACTTGAAATAGCAGACCAGCTGCAGGGGCTGAGC
CTTCAGGAGATGCCTGGAGATGTCCCCCTGGCCCGCATCCAGCGCCTCTTTTCCTTCAGG
GCTTTGGAATCTGGCCACTTCCCCCAGCCTGAAAAGGAGAGTTTCCAGGAGCGCCTGGCT
CTGATCCCCAGTGGGGTGACTGTGTGTGTGTTGGCCCTGGCCACCCTCCAGCCCGGAACC
GTGGGCAACACCCTCCTGCTGACCCGGCTGGAAAAGGACAGTCCCCCAGTCAGTGTGCAG
ATTCCCACTGGCCAGAACAAGCTTCATCTGCGTTCAGTCCTGAATGAGTTTGATGCCATC
CAGAAGGCACAGAAAGAGAACAGCAGCTGTACTGACAAGCGAGAATGGTGGACAGGGCGG
CTGGCACTGGACCACAGGATGGAGGTTCTCATCGCTTCCCTAGAGAAGTCTGTGCTGGGC
TGCTGGAAGGGGCTGCTGCTGCCGTCCAGTGAGGAGCCCGGCCCTGCCCAGGAGGCCTCC
CGCCTACAGGAGCTGCTACAGGACTGTGGCTGGAAATATCCTGACCGCACTCTGCTGAAA
ATCATGCTCAGTGGTGCCGGTGCCCTCACCCCTCAGGACATTCAGGCCCTGGCCTACGGG
CTGTGCCCAACCCAGCCAGAGCGAGCCCAGGAGCTCCTGAATGAGGCAGTAGGACGTCTA
CAGGGCCTGACAGTACCAAGCAATAGCCACCTTGTCTTGGTCCTAGACAAGGACTTGCAG
AAGCTGCCGTGGGAAAGCATGCCCAGCCTCCAAGCACTGCCTGTCACCCGGCTGCCCTCC
TTCCGCTTCCTACTCAGCTACTCCATCATCAAAGAGTATGGGGCCTCGCCAGTGCTGAGT
CAAGGGGTGGATCCACGAAGTACCTTCTATGTCCTGAACCCTCACAATAACCTGTCAAGC
ACAGAGGAGCAATTTCGAGCCAATTTCAGCAGTGAAGCTGGCTGGAGAGGAGTGGTTGGG
GAGGTGCCAAGACCTGAACAGGTGCAGGAAGCCCTGACAAAGCATGATTTGTATATCTAT
GCAGGGCATGGGGCTGGTGCCCGCTTCCTTGATGGGCAGGCTGTCCTGCGGCTGAGCTGT
CGGGCAGTGGCCCTGCTGTTTGGCTGTAGCAGTGCGGCCCTGGCTGTGCGTGGAAACCTG
GAGGGGGCTGGCATCGTGCTCAAGTACATCATGGCTGGTTGCCCCTTGTTTCTGGGTAAT
CTCTGGGATGTGACTGACCGCGACATTGACCGCTACACGGAAGCTCTGCTGCAAGGCTGG
CTTGGAGCAGGCCCAGGGGCCCCCCTTCTCTACTATGTAAACCAGGCCCGCCAAGCTCCC
CGACTCAAGTATCTTATTGGGGCTGCACCTATAGCCTATGGCTTGCCTGTCTCTCTGCGG
Protein sequence predicted based on nucleotide sequence
MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQ LTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRL AQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLED ESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSS GLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKL LQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAI LSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDL ADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAIS EPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPE HMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTG QERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLL DSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYE DKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILA ALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAA SSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLL RVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILS TQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTK LQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQ KGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFL SHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQ ALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPH LEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGR QKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQA PRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVV APGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKM SFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRL RLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLV TESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFR ALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQ IPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLG CWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYG LCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPS FRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVG EVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNL EGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAP RLKYLIGAAPIAYGLPVSLR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
