Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00942106
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 468nts
Open reading frame: 28 to 495
GCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTCGTGAAAACCCTTACGGGGAAG
ACCATCACCCTCGAGGTTGAACCCTCGGATACGATAGAAAATGTAAAGGCCAAGATCCAG
GATAAGGAAGGAATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGCAGCTGGAA
GATGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATCTTGTGTTG
AGACTTCGTGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTTACACCACTCCCAAGAAGAAT
AAGCACAAGAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGGATGAGAAT
GGCAAAATTAGTCGCCTTCGTCGAGAGTGCCCTTCTGATGAATGTGGTGCTGGGGTGTTT
ATGGCAAGTCACTTTGACAGACATTATTGTGGCAAATGTTGTCTGACTTACTGTTTCAAC
AAACCAGAAGACAAGTAACTGTATGAGTTAATAAAAGACATGAACTAAAAAAAAAAAAAA
AAAA
Protein sequence predicted based on nucleotide sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRE CPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Host Type: mammalian Marker: blasticidin
Host Type: bacterial Marker: chloramphenicol
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLenti6.3/V5-DEST
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
V5 reverse
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection, mammalian expression expression with RSV and 5pLTR and HIV-1 psi and RRE and gp41 peptide and cPPT and CMV Promoter and CMV enhancer and attR1 and lac UV5 promoter and CmR and ccdB and attR2 and V5 and WPRE and Factor Xa site and SV40 promoter and SV40 Ori and T7 promoter and M13 forward and f1 ori and AmpR promoter and AmpR and ori and CAP binding site and lac promoter and lac operator and M13 reverse and T3 and EM7 and BlastR and 3pLTR and SV40 poly(A) signal; ampicillin resistance in bacterial, chloramphenicol resistance in bacterial, blasticidin resistance in mammalian;
Comments:
pLent6.3/V5-DEST from Invitrogen: https://www.thermofisher.com/order/catalog/product/V53306
Catalog: V53306
For research use only. Not intended for any therapeutic or diagnostic use.
The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway destination vector for expression of a C-terminally V5-tagged protein.
Size (bp):
9387
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
Invitrogen
Publications:
None
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
V5
epitope tag from simian virus 5
4325
4366
CDS
gp41 peptide
antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)
1493
1537
CDS
CmR
confers resistance to chloramphenicol
2801
3460
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
3802
4107
CDS
Factor Xa site
Factor Xa recognition and cleavage site
4861
4872
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
7302
8162
Miscellaneous Feature
WPRE
woodchuck hepatitis virus posttranscriptional regulatory element
4390
4978
Miscellaneous Feature
cPPT
central polypurine tract and central termination sequence of HIV-1
1804
1921
Miscellaneous Feature
HIV-1 psi
packaging signal of human immunodeficiency virus type 1
457
582
Miscellaneous Feature
RRE
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
1075
1308
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
1944
2247
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
6558
6574
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
9307
9323
promoter
lac UV5 promoter
E. coli lac promoter with an "up" mutation
2717
2747
promoter
SV40 promoter
SV40 enhancer and early promoter
4993
5309
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
6530
6548
promoter
CMV Promoter
human cytomegalovirus (CMV) immediate early promoter
2248
2451
promoter
RSV
Rous sarcoma virus enhancer/promoter
3
229
promoter
AmpR promoter
null
7197
7301
promoter
lac promoter
promoter for the E. coli lac operon
9245
9275
promoter
T3
promoter for bacteriophage T3 RNA polymerase
9344
9362
protein binding site
attR1
recombination site for the Gateway LR reaction
2568
2692
protein binding site
attR2
recombination site for the Gateway LR reaction
4148
4272
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
9209
9230
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
9283
9299
rep_origin
SV40 Ori
SV40 origin of replication
5160
5295
rep_origin
f1 ori
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
6716
7171
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
8333
8921
viral LTR
5pLTR
truncated 5' long terminal repeat (LTR) from HIV-1
230
410
Gene
Gene Symbol :
RPS27A
Symbol Nomenclature :
RPS27A
SYNONYM :
CEP80; HEL112; S27A; UBA80; UBC; UBCEP1; UBCEP80
Designation :
40S ribosomal protein S27a|epididymis luminal protein 112|ubiquitin C|ubiquitin and ribosomal protein S27a|ubiquitin carboxyl extension protein 80|ubiquitin-40S ribosomal protein S27a|ubiquitin-CEP80
Full Nomenclature :
ribosomal protein S27a
GENEID :
GenBank Accession :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
Reactome :
3' -UTR-mediated translational regulation
Reactome :
Activated NOTCH1 Transmits Signal to the Nucleus
Reactome :
Activated TLR4 signalling
Reactome :
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon
Reactome :
Activation of NF-kappaB in B cells
Reactome :
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
Reactome :
Adaptive Immune System
Reactome :
Antigen processing-Cross presentation
Reactome :
Antigen processing: Ubiquitination & Proteasome degradation
Reactome :
Antiviral mechanism by IFN-stimulated genes
Reactome :
APC-Cdc20 mediated degradation of Nek2A
Reactome :
APC/C-mediated degradation of cell cycle proteins
Reactome :
APC/C:Cdc20 mediated degradation of Cyclin B
Reactome :
APC/C:Cdc20 mediated degradation of mitotic proteins
Reactome :
APC/C:Cdc20 mediated degradation of Securin
Reactome :
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
Reactome :
Apoptosis
Reactome :
Assembly Of The HIV Virion
Reactome :
Assembly of the pre-replicative complex
Reactome :
Association of licensing factors with the pre-replicative complex
Reactome :
Asymmetric localization of PCP proteins
Reactome :
AUF1 (hnRNP D0) destabilizes mRNA
Reactome :
Autodegradation of Cdh1 by Cdh1:APC/C
Reactome :
Autodegradation of the E3 ubiquitin ligase COP1
Reactome :
beta-catenin independent WNT signaling
Reactome :
Budding and maturation of HIV virion
Reactome :
Cap-dependent Translation Initiation
Reactome :
Cdc20:Phospho-APC/C mediated degradation of Cyclin A
Reactome :
CDK-mediated phosphorylation and removal of Cdc6
Reactome :
CDT1 association with the CDC6:ORC:origin complex
Reactome :
Cell Cycle
Reactome :
Cell Cycle Checkpoints
Reactome :
Cell Cycle, Mitotic
Reactome :
Cell death signalling via NRAGE, NRIF and NADE
Reactome :
Cellular response to hypoxia
Reactome :
Cellular responses to stress
Reactome :
Cellular Senescence
Reactome :
Circadian Clock
Reactome :
Class I MHC mediated antigen processing & presentation
Reactome :
Constitutive Signaling by NOTCH1 HD Domain Mutants
Reactome :
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants
Reactome :
Constitutive Signaling by NOTCH1 PEST Domain Mutants
Reactome :
Cyclin A:Cdk2-associated events at S phase entry
Reactome :
Cyclin D associated events in G1
Reactome :
Cyclin E associated events during G1/S transition
Reactome :
Cytokine Signaling in Immune system
Reactome :
Cytosolic sensors of pathogen-associated DNA
Reactome :
deactivation of the beta-catenin transactivating complex
Reactome :
degradation of AXIN
Reactome :
degradation of DVL
Reactome :
Disease
Reactome :
DNA Repair
Reactome :
DNA Replication
Reactome :
DNA Replication Pre-Initiation
Reactome :
Downregulation of ERBB2:ERBB3 signaling
Reactome :
Downregulation of ERBB4 signaling
Reactome :
Downregulation of SMAD2/3:SMAD4 transcriptional activity
Reactome :
Downregulation of TGF-beta receptor signaling
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
EGFR downregulation
Reactome :
Endosomal Sorting Complex Required For Transport (ESCRT)
Reactome :
ER-Phagosome pathway
Reactome :
Eukaryotic Translation Elongation
Reactome :
Eukaryotic Translation Initiation
Reactome :
Eukaryotic Translation Termination
Reactome :
Fanconi Anemia pathway
Reactome :
FBXW7 Mutants and NOTCH1 in Cancer
Reactome :
Formation of a pool of free 40S subunits
Reactome :
Formation of the ternary complex, and subsequently, the 43S complex
Reactome :
G1 Phase
Reactome :
G1/S DNA Damage Checkpoints
Reactome :
G1/S Transition
Reactome :
G2/M Transition
Reactome :
Gene Expression
Reactome :
Generic Transcription Pathway
Reactome :
Glucose metabolism
Reactome :
Glycogen storage diseases
Reactome :
Glycogen synthesis
Reactome :
GTP hydrolysis and joining of the 60S ribosomal subunit
Reactome :
HIV Infection
Reactome :
HIV Life Cycle
Reactome :
Host Interactions of HIV factors
Reactome :
IKK complex recruitment mediated by RIP1
Reactome :
Immune System
Reactome :
Influenza Infection
Reactome :
Influenza Life Cycle
Reactome :
Influenza Viral RNA Transcription and Replication
Reactome :
Innate Immune System
Reactome :
Interferon Signaling
Reactome :
Ion channel transport
Reactome :
IRAK2 mediated activation of TAK1 complex
Reactome :
IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation
Reactome :
ISG15 antiviral mechanism
Reactome :
L13a-mediated translational silencing of Ceruloplasmin expression
Reactome :
Late Phase of HIV Life Cycle
Reactome :
Loss of Function of SMAD2/3 in Cancer
Reactome :
Loss of Function of SMAD4 in Cancer
Reactome :
Loss of Function of TGFBR1 in Cancer
Reactome :
Loss of Function of TGFBR2 in Cancer
Reactome :
M Phase
Reactome :
M/G1 Transition
Reactome :
Membrane binding and targetting of GAG proteins
Reactome :
Membrane Trafficking
Reactome :
Metabolism
Reactome :
Metabolism of carbohydrates
Reactome :
Metabolism of mRNA
Reactome :
Metabolism of proteins
Reactome :
Metabolism of RNA
Reactome :
Mitotic Anaphase
Reactome :
Mitotic G1-G1/S phases
Reactome :
Mitotic G2-G2/M phases
Reactome :
Mitotic M-M/G1 phases
Reactome :
Mitotic Metaphase and Anaphase
Reactome :
MyD88 cascade initiated on plasma membrane
Reactome :
MyD88 dependent cascade initiated on endosome
Reactome :
MyD88-independent cascade
Reactome :
MyD88:Mal cascade initiated on plasma membrane
Reactome :
Myoclonic epilepsy of Lafora
Reactome :
Negative regulation of FGFR signaling
Reactome :
Negative regulators of RIG-I/MDA5 signaling
Reactome :
NF-kB is activated and signals survival
Reactome :
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)
Reactome :
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
Reactome :
Nonsense-Mediated Decay (NMD)
Reactome :
NOTCH1 Intracellular Domain Regulates Transcription
Reactome :
NOTCH2 Activation and Transmission of Signal to the Nucleus
Reactome :
NRIF signals cell death from the nucleus
Reactome :
Oncogene Induced Senescence
Reactome :
Orc1 removal from chromatin
Reactome :
Oxidative Stress Induced Senescence
Reactome :
Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
Reactome :
p53-Dependent G1 DNA Damage Response
Reactome :
p53-Dependent G1/S DNA damage checkpoint
Reactome :
p53-Independent DNA Damage Response
Reactome :
p53-Independent G1/S DNA damage checkpoint
Reactome :
p75 NTR receptor-mediated signalling
Reactome :
p75NTR recruits signalling complexes
Reactome :
p75NTR signals via NF-kB
Reactome :
PCP/CE pathway
Reactome :
Peptide chain elongation
Reactome :
Receptor-ligand binding initiates the second proteolytic cleavage of Notch receptor
Reactome :
Regulation of activated PAK-2p34 by proteasome mediated degradation
Reactome :
Regulation of APC/C activators between G1/S and early anaphase
Reactome :
Regulation of Apoptosis
Reactome :
Regulation of DNA replication
Reactome :
regulation of FZD by ubiquitination
Reactome :
Regulation of Hypoxia-inducible Factor (HIF) by oxygen
Reactome :
Regulation of innate immune responses to cytosolic DNA
Reactome :
Regulation of mitotic cell cycle
Reactome :
Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome :
Regulation of PLK1 Activity at G2/M Transition
Reactome :
Regulation of the Fanconi anemia pathway
Reactome :
Removal of licensing factors from origins
Reactome :
Ribosomal scanning and start codon recognition
Reactome :
RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways
Reactome :
S Phase
Reactome :
SCF(Skp2)-mediated degradation of p27/p21
Reactome :
SCF-beta-TrCP mediated degradation of Emi1
Reactome :
Senescence-Associated Secretory Phenotype (SASP)
Reactome :
Separation of Sister Chromatids
Reactome :
Signal Transduction
Reactome :
Signaling by constitutively active EGFR
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by NOTCH
Reactome :
Signaling by NOTCH1
Reactome :
Signaling by NOTCH1 HD Domain Mutants in Cancer
Reactome :
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer
Reactome :
Signaling by NOTCH1 in Cancer
Reactome :
Signaling by NOTCH1 PEST Domain Mutants in Cancer
Reactome :
Signaling by NOTCH1 t(7;9)(NOTCH1:M1580_K2555) Translocation Mutant
Reactome :
Signaling by NOTCH2
Reactome :
Signaling by TGF-beta Receptor Complex
Reactome :
Signaling by TGF-beta Receptor Complex in Cancer
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
SMAD2/3 MH2 Domain Mutants in Cancer
Reactome :
SMAD2/3 Phosphorylation Motif Mutants in Cancer
Reactome :
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
Reactome :
SMAD4 MH2 Domain Mutants in Cancer
Reactome :
Spry regulation of FGF signaling
Reactome :
SRP-dependent cotranslational protein targeting to membrane
Reactome :
Stabilization of p53
Reactome :
Stimuli-sensing channels
Reactome :
Switching of origins to a post-replicative state
Reactome :
Synthesis of DNA
Reactome :
TCF dependent signaling in response to WNT
Reactome :
TGF-beta receptor signaling activates SMADs
Reactome :
TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
Reactome :
TGFBR1 KD Mutants in Cancer
Reactome :
TGFBR1 LBD Mutants in Cancer
Reactome :
TGFBR2 Kinase Domain Mutants in Cancer
Reactome :
TGFBR2 MSI Frameshift Mutants in Cancer
Reactome :
Toll Like Receptor 10 (TLR10) Cascade
Reactome :
Toll Like Receptor 2 (TLR2) Cascade
Reactome :
Toll Like Receptor 3 (TLR3) Cascade
Reactome :
Toll Like Receptor 4 (TLR4) Cascade
Reactome :
Toll Like Receptor 5 (TLR5) Cascade
Reactome :
Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome :
Toll Like Receptor 9 (TLR9) Cascade
Reactome :
Toll Like Receptor TLR1:TLR2 Cascade
Reactome :
Toll Like Receptor TLR6:TLR2 Cascade
Reactome :
Toll-Like Receptors Cascades
Reactome :
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome :
TRAF6 Mediated Induction of proinflammatory cytokines
Reactome :
TRAF6 mediated induction of TAK1 complex
Reactome :
Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer
Reactome :
Translation
Reactome :
Translation initiation complex formation
Reactome :
Transmembrane transport of small molecules
Reactome :
TRIF-mediated TLR3/TLR4 signaling
Reactome :
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
Reactome :
Ubiquitin-dependent degradation of Cyclin D
Reactome :
Ubiquitin-dependent degradation of Cyclin D1
Reactome :
Vif-mediated degradation of APOBEC3G
Reactome :
Viral mRNA Translation
Reactome :
Vpu mediated degradation of CD4
WikiPathway :
Cytoplasmic Ribosomal Proteins
Protein
HPRD :
01878
Clone
Cloning Information :
Organism
Species Specific ID:
Genome