Gene:
Gene Symbol:
TRIO
Gene Name:
trio Rho guanine nucleotide exchange factor
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJFT7_nHalo_DC(r4)
Format:
CLOSED (STOP codon in vector)
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946217
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 9294nts
Open reading frame: 1 to 9294
ATGAGCGGCAGCAGCGGCGGAGCCGCCGCCCCCGCCGCGTCCTCCGGCCCCGCCGCGGCG
GCCAGCGCGGCTGGCTCGGGCTGCGGGGGCGGTGCCGGCGAGGGGGCAGAGGAGGCGGCC
AAGGACCTGGCCGACATCGCGGCCTTCTTCCGATCCGGGTTTCGAAAAAACGATGAAATG
AAAGCTATGGATGTTTTACCAATTTTGAAGGAAAAAGTTGCATACCTTTCAGGTGGGAGA
GATAAACGTGGAGGTCCCATTTTAACGTTTCCGGCCCGCAGCAATCATGACAGAATACGA
CAGGAGGATCTCAGGAGACTCATTTCCTATCTAGCCTGTATTCCCAGCGAGGAGGTCTGC
AAGCGTGGCTTCACGGTGATCGTGGACATGCGTGGGTCCAAGTGGGACTCCATCAAGCCC
CTTCTGAAGATCCTGCAGGAGTCCTTCCCCTGCTGCATCCATGTGGCCCTGATCATCAAG
CCAGACAACTTCTGGCAGAAACAGAGGACTAATTTTGGCAGTTCTAAATTTGAATTTGAG
ACAAATATGGTCTCTTTAGAAGGCCTTACCAAAGTAGTTGATCCTTCTCAGCTAACTCCT
GAGTTTGATGGCTGCCTGGAATACAACCACGAAGAATGGATTGAAATCAGAGTTGCTTTT
GAAGACTACATTAGCAATGCCACCCACATGCTGTCTCGGCTGGAGGAACTTCAGGACATC
CTAGCTAAGAAGGAGCTGCCTCAGGATTTAGAGGGGGCTCGGAATATGATCGAGGAACAT
TCTCAGCTGAAGAAGAAGGTGATTAAGGCCCCCATCGAGGACCTGGATTTGGAGGGACAG
AAGCTGCTTCAGAGGATACAGAGCAGTGAAAGCTTTCCCAAAAAGAACTCAGGCTCAGGC
AATGCGGACCTGCAGAACCTCTTGCCCAAGGTGTCCACCATGCTGGACCGGCTGCACTCG
ACACGGCAGCATCTGCACCAGATGTGGCATGTGAGGAAGCTGAAGCTGGACCAGTGCTTC
CAGCTGAGGCTGTTTGAACAGGATGCTGAGAAGATGTTTGACTGGATCACACACAACAAA
GGCCTGTTTCTAAACAGCTACACAGAGATTGGGACCAGCCACCCTCATGCCATGGAGCTT
CAGACGCAGCACAATCACTTTGCCATGAACTGTATGAACGTGTATGTAAATATAAACCGC
ATCATGTCGGTGGCCAATCGTCTGGTGGAGTCTGGCCACTATGCCTCGCAGCAGATCAGG
CAGATCGCGAGTCAGCTGGAGCAGGAGTGGAAGGCGTTTGCGGCAGCCCTGGATGAGCGG
AGCACCTTGCTGGACATGTCCTCCATTTTCCACCAGAAGGCCGAAAAGTATATGAGCAAC
GTGGATTCATGGTGTAAAGCTTGCGGTGAGGTAGACCTTCCCTCAGAGCTGCAGGACCTA
GAAGATGCCATTCATCACCACCAGGGAATATATGAACATATCACTCTTGCTTATTCTGAG
GTCAGCCAAGATGGGAAGTCGCTCCTTGACAAGCTCCAGCGGCCCTTGACTCCCGGCAGC
TCCGATTCCCTGACAGCCTCTGCCAACTACTCCAAGGCCGTGCACCATGTCCTGGATGTC
ATCCACGAGGTGCTGCACCACCAGCGGCAGCTGGAGAACATCTGGCAACACCGCAAGGTC
CGGCTGCATCAGAGGCTGCAGCTGTGTGTTTTCCAGCAGGACGTTCAGCAGGTGCTAGAC
TGGATCGAGAACCACGGAGAAGCATTTCTGAGCAAACATACAGGTGTGGGGAAATCTCTT
CATCGGGCCAGAGCATTGCAGAAACGTCATGAAGATTTTGAAGAAGTGGCACAGAACACA
TACACCAATGCGGATAAATTACTGGAAGCAGCAGAACAGCTGGCTCAGACTGGGGAATGT
GACCCCGAAGAGATTTATCAGGCTGCCCATCAGCTGGAAGACCGGATTCAAGATTTCGTT
CGGCGTGTTGAGCAGCGAAAGATCCTACTGGACATGTCAGTGTCCTTTCACACCCATGTG
AAAGAGCTGTGGACGTGGCTGGAGGAGCTGCAGAAGGAGCTGCTGGACGACGTGTATGCC
GAGTCGGTGGAGGCCGTGCAGGACCTCATCAAGCGCTTTGGCCAGCAGCAGCAGACCACC
CTGCAGGTGACTGTCAACGTGATCAAGGAAGGGGAGGACCTCATCCAGCAGCTCAGGGAC
TCTGCCATCTCCAGTAACAAGACCCCCCACAACAGCTCCATCAACCACATTGAGACGGTG
CTGCAGCAGCTGGACGAGGCGCAGTCGCAGATGGAGGAGCTCTTCCAGGAGCGCAAGATC
AAGCTGGAGCTCTTCCTGCAGCTGCGCATCTTCGAGAGGGACGCCATCGACATTATCTCA
GACCTCGAGTCTTGGAATGATGAGCTTTCTCAGCAAATGAATGACTTCGACACAGAAGAT
CTCACGATTGCAGAGCAGCGCCTCCAGCACCATGCAGACAAAGCCTTGACCATGAACAAC
TTGACTTTTGACGTCATCCACCAAGGGCAAGATCTTCTGCAGTATGTCAATGAGGTCCAG
GCCTCTGGTGTGGAGCTGCTGTGTGATAGAGATGTAGACATGGCAACTCGGGTCCAGGAC
CTGCTGGAGTTTCTTCATGAAAAACAGCAGGAATTGGATTTAGCCGCAGAGCAGCATCGG
AAACACCTGGAGCAGTGCGTGCAGCTGCGCCACCTGCAGGCAGAAGTGAAACAGGTGCTG
GGTTGGATCCGCAACGGAGAGTCCATGTTAAATGCCGGACTTATCACAGCCAGCTCGTTA
CAAGAGGCAGAGCAGCTCCAGCGAGAGCACGAGCAGTTCCAGCATGCCATTGAGAAAACA
CATCAGAGCGCGCTGCAGGTGCAGCAGAAGGCAGAAGCCATGCTACAGGCCAACCACTAC
GACATGGACATGATCCGGGACTGCGCCGAGAAGGTGGCGTCTCACTGGCAACAGCTCATG
CTCAAGATGGAAGATCGCCTCAAGCTCGTCAACGCCTCTGTCGCTTTCTACAAAACCTCA
GAGCAGGTCTGCAGCGTCCTCGAGAGCCTGGAACAGGAGTACAAGAGAGAAGAAGACTGG
TGTGGCGGGGCGGATAAGCTGGGCCCAAACTCTGAGACGGACCACGTGACGCCCATGATC
AGCAAGCACCTGGAGCAGAAGGAGGCATTCCTGAAGGCTTGCACCCTTGCTCGGAGGAAT
GCAGACGTCTTCCTGAAATACCTGCACAGGAACAGCGTGAACATGCCAGGAATGGTGACG
CACATCAAAGCTCCTGAACAGCAAGTGAAAAATATCTTGAATGAACTCTTCCAACGGGAG
AACAGGGTATTGCATTACTGGACCATGAGGAAGAGACGGCTGGACCAGTGTCAGCAGTAC
GTGGTCTTTGAGAGGAGTGCCAAGCAGGCTTTGGAATGGATCCATGACAATGGCGAGTTC
TACCTTTCCACACACACCTCCACGGGCTCCAGTATACAGCACACCCAGGAGCTCCTGAAA
GAGCACGAGGAGTTCCAGATAACTGCAAAGCAAACCAAAGAGAGAGTGAAGCTATTGATA
CAGCTGGCTGATGGCTTTTGTGAAAAAGGGCATGCCCATGCGGCAGAGATAAAAAAATGT
GTTACTGCTGTGGATAAGAGGTACAGAGATTTCTCTCTGCGGATGGAGAAGTACAGGACC
TCTTTGGAAAAAGCCCTGGGGATTTCTTCAGATTCCAACAAATCGAGTAAAAGTCTCCAG
CTAGATATCATTCCAGCCAGTATCCCTGGCTCAGAGGTGAAACTTCGAGATGCTGCTCAT
GAACTTAATGAAGAGAAGCGGAAATCTGCCCGCAGGAAAGAGTTCATAATGGCTGAGCTC
ATTCAAACTGAAAAGGCTTATGTAAGAGACCTCCGGGAATGTATGGATACGTACCTGTGG
GAAATGACCAGTGGCGTGGAAGAGATTCCACCTGGCATTGTAAACAAAGAACTCATCATC
TTCGGAAACATGCAAGAAATCTACGAATTTCATAATAACATATTCCTAAAGGAGCTGGAA
AAATATGAACAGTTGCCAGAGGATGTTGGACATTGTTTTGTTACTTGGGCAGACAAGTTT
CAGATGTATGTCACATATTGCAAAAATAAGCCTGATTCTACTCAGCTGATATTGGAACAT
GCAGGGTCCTATTTTGACGAGATACAGCAGCGACATGGATTAGCCAATTCCATTTCTTCC
TACCTTATTAAACCAGTTCAGCGAATAACGAAGTATCAGCTCCTTTTAAAAGAGCTGCTG
ACGTGCTGTGAGGAAGGAAAGGGAGAGATTAAAGATGGCCTGGAGGTGATGCTCAGCGTG
CCGAAGCGAGCCAATGATGCCATGCACCTCAGCATGCTGGAAGGGTTTGATGAAAACATT
GAGTCTCAGGGAGAACTCATCCTACAGGAATCCTTCCAAGTGTGGGACCCAAAAACCTTA
ATTCGAAAGGGTCGAGAACGGCATCTCTTCCTTTTTGAAATGTCCTTAGTATTTAGTAAA
GAAGTGAAAGATTCCAGTGGGAGAAGCAAGTACCTTTATAAAAGCAAATTGTTTACCTCA
GAGTTGGGTGTCACAGAACATGTTGAAGGAGACCCTTGCAAATTTGCACTGTGGGTGGGG
AGAACACCAACTTCAGATAATAAAATTGTCCTTAAGGCTTCCAGCATAGAGAACAAGCAG
GACTGGATAAAGCATATCCGCGAAGTCATCCAGGAGCGGACGATCCACCTGAAGGGAGCC
CTGAAGGAGCCCATTCACATCCCTAAGACCGCTCCCGCCACAAGACAGAAGGGAAGGAGG
GATGGAGAGGATCTGGACAGCCAAGGAGACGGCAGCAGCCAGCCTGATACGATTTCCATC
GCCTCACGGACGTCTCAGAACACGCTGGACAGCGATAAGCTCTCTGGTGGCTGTGAGCTG
ACAGTGGTGATCCATGACTTCACCGCTTGCAACAGCAACGAGCTGACCATCCGACGGGGC
CAGACCGTGGAAGTTCTGGAGCGGCCGCATGACAAGCCTGACTGGTGTCTGGTGCGGACA
ACTGACCGCTCCCCAGCGGCAGAAGGCCTGGTCCCCTGTGGTTCACTGTGCATCGCCCAC
TCCAGAAGTAGCATGGAAATGGAGGGCATCTTCAACCACAAAGACTCGCTCTCCGTCTCC
AGCAATGACGCCAGTCCACCCGCATCCGTGGCTTCCCTCCAGCCCCACATGATCGGGGCC
CAGAGCTCGCCGGGCCCCAAGCGGCCGGGCAACACCCTGCGCAAGTGGCTCACCAGCCCC
GTGCGGCGGCTCAGCAGCGGCAAGGCCGACGGGCACGTGAAGAAGCTGGCGCACAAGCAC
AAGAAGAGCCGCGAGGTCCGCAAGAGCGCCGACGCCGGCTCGCAGAAGGACTCCGACGAC
AGTGCGGCCACCCCGCAGGACGAGACGGTCGAGGAGAGAGGCCGGAACGAGGGCCTGAGC
AGCGGTACTCTCTCCAAATCCTCCTCCTCGGGGATGCAGAGCTGTGGAGAAGAGGAAGGC
GAGGAGGGGGCCGACGCCGTGCCCCTGCCGCCACCCATGGCCATCCAGCAGCACAGCCTC
CTCCAGCCAGACTCACAGGATGACAAGGCCTCTTCTCGGTTATTAGTCCGCCCCACCAGC
TCCGAAACACCGAGTGCAGCCGAGCTCGTCAGTGCAATTGAGGAACTCGTGAAAAGCAAG
ATGGCACTGGAGGATCGCCCCAGCTCACTCCTTGTTGACCAGGGAGATAGTAGCAGCCCT
TCCTTCAACCCTTCGGATAATTCCCTTCTCTCTTCCTCCTCGCCCATTGATGAGATGGAA
GAAAGGAAATCCAGCTCTTTAAAGAGAAGACACTACGTTTTGCAAGAACTAGTGGAGACA
GAGCGTGACTATGTGCGGGACCTTGGCTATGTGGTTGAGGGCTACATGGCACTTATGAAA
GAAGATGGTGTTCCTGATGACATGAAAGGAAAAGACAAAATTGTGTTCGGCAACATCCAT
CAGATTTACGACTGGCACAGAGACTTTTTTTTAGGAGAGTTAGAGAAGTGCCTTGAAGAT
CCAGAAAAACTAGGATCCCTTTTTGTTAAACACGAGAGAAGGTTGCACATGTACATAGCT
TATTGTCAAAATAAACCAAAGTCTGAGCACATTGTCTCAGAATACATTGATACCTTTTTT
GAGGACTTAAAGCAGCGTCTTGGCCACAGGTTACAGCTCACAGATCTGTTGATCAAACCA
GTGCAGAGAATCATGAAGTATCAGCTGTTACTGAAGGACTTCCTCAAGTATTCCAAAAAG
GCCAGCCTGGATACATCAGAATTAGAGAGAGCTGTGGAAGTCATGTGCATAGTACCCAGG
CGGTGCAACGACATGATGAACGTGGGGCGGCTGCAAGGATTCGACGGGAAAATCGTTGCC
CAGGGTAAACTGCTCTTGCAGGACACATTCTTGGTCACAGACCAAGATGCAGGACTTCTG
CCTCGCTGCAGAGAGAGGCGCATCTTCCTCTTTGAGCAGATCGTCATATTCAGCGAACCA
CTTGATAAAAAGAAGGGCTTCTCCATGCCGGGATTCCTGTTTAAGAACAGTATCAAGGTG
AGTTGCCTTTGCCTGGAGGAAAATGTGGAAAATGATCCCTGTAAATTTGCTCTGACATCG
AGGACGGGTGACGTGGTAGAGACCTTCATTTTGCATTCATCTAGTCCAAGTGTCCGGCAA
ACTTGGATCCATGAAATCAACCAAATTTTAGAAAACCAGCGCAATTTTTTAAATGCCTTG
ACATCGCCAATCGAGTACCAGAGGAACCACAGCGGGGGCGGCGGCGGCGGCGGCAGCGGG
GGCAGCGGCGGGGGTGGGGGCAGCGGCGGCGGCGGGGCCCCCAGTGGCGGCAGCGGCCAC
AGTGGCGGCCCCAGCAGCTGCGGCGGCGCCCCCAGCACGAGCAGGAGCCGGCCCTCCCGG
ATCCCCCAGCCTGTCCGACACCACCCCCCCGTGCTGGTCTCCTCTGCAGCCTCGAGCCAG
GCAGAGGCAGACAAGATGTCAGGTACGTCCACCCCCGGGCCCTCCCTGCCTCCCCCTGGC
GCGGCCCCCGAGGCCGGCCCCAGCGCGCCCAGCAGGCGGCCCCCCGGCGCGGACGCCGAG
GGGTCCGAGCGAGAAGCGGAGCCGATCCCCAAGATGAAGGTGCTGGAGAGCCCCAGGAAA
GGCGCCGCGAACGCCTCGGGGTCGAGCCCAGACGCCCCCGCCAAGGACGCGCGCGCTAGC
CTGGGCACCCTGCCGCTTGGGAAGCCCCGGGCCGGGGCCGCTTCGCCGCTGAACTCGCCG
CTCTCCAGCGCGGTCCCTTCTCTCGGCAAGGAGCCCTTCCCCCCCAGCAGCCCCCTGCAG
AAGGGGGGCTCCTTCTGGAGCTCCATCCCCGCCTCCCCCGCCAGCCGACCCGGCTCCTTC
ACCTTCCCGGGGGACAGCGACTCCCTCCAGCGGCAGACACCCCGCCACGCGGCCCCTGGC
AAGGATACTGACCGCATGAGCACGTGCTCCTCGGCCAGCGAGCAGTCCGTGCAGTCCACC
CAGAGCAACGGGAGTGAAAGCAGCAGCAGTAGCAACATCTCCACCATGTTGGTGACACAC
GATTACACGGCAGTGAAGGAGGATGAGATCAACGTCTACCAAGGAGAGGTCGTTCAAATT
CTGGCCAGCAACCAGCAGAACATGTTTCTGGTGTTCCGAGCCGCCACTGACCAGTGCCCC
GCAGCTGAGGGCTGGATTCCAGGCTTTGTCCTGGGCCACACCAGTGCAGTCATCGTGGAG
AACCCGGACGGGACTCTCAAGAAGTCAACATCTTGGCACACAGCACTCCGTTTAAGGAAA
AAATCTGAGAAAAAAGATAAAGACGGCAAAAGGGAAGGCAAGTTAGAGAACGGTTATCGG
AAGTCACGGGAAGGACTCAGCAACAAGGTATCTGTGAAGCTTCTCAATCCCAACTACATT
TATGACGTTCCCCCAGAATTCGTCATTCCATTGAGTGAGGTCACGTGTGAGACAGGGGAG
ACCGTTGTTCTTAGATGTCGAGTCTGTGGCCGCCCCAAAGCCTCAATTACCTGGAAGGGC
CCTGAACACAACACCTTGAACAACGATGGTCACTACAGCATCTCCTACAGTGACCTGGGA
GAGGCCACGCTGAAGATTGTGGGCGTGACCACGGAAGATGACGGCATCTACACGTGCATC
GCTGTCAATGACATGGGTTCAGCCTCATCGTCGGCCAGCCTGAGGGTCCTAGGTCCAGGG
ATGGATGGGATCATGGTGACCTGGAAAGACAACTTTGACTCCTTCTACAGTGAAGTGGCT
GAGCTTGGCAGGGGCAGATTCTCTGTCGTTAAGAAATGTGATCAGAAAGGAACCAAGCGA
GCAGTGGCCACTAAGTTTGTGAACAAGAAGTTGATGAAGCGCGACCAGGTCACCCATGAG
CTTGGCATCCTGCAGAGCCTCCAGCACCCCCTGCTTGTCGGCCTCCTCGACACCTTTGAG
ACCCCCACCAGCTACATCCTGGTCTTAGAAATGGCTGACCAGGGTCGCCTCCTGGACTGC
GTGGTGCGATGGGGAAGCCTCACTGAAGGGAAGATCAGGGCGCACCTGGGGGAGGTTCTG
GAAGCTGTCCGGTACCTGCACAACTGCAGGATAGCACACCTGGACCTAAAGCCTGAGAAT
ATCCTGGTGGATGAGAGTTTAGCCAAGCCAACCATCAAACTGGCTGACTTTGGAGATGCT
GTTCAGCTCAACACGACCTACTACATCCACCAGTTACTGGGGAACCCTGAATTCGCAGCC
CCTGAAATCATCCTCGGGAACCCTGTCTCCCTGACCTCGGATACGTGGAGTGTTGGAGTG
CTCACATACGTACTTCTTAGTGGCGTGTCCCCCTTCCTGGATGACAGTGTGGAAGAGACC
TGCCTGAACATTTGCCGCTTAGACTTTAGCTTCCCAGATGACTACTTTAAAGGAGTGAGC
CAGAAGGCCAAGGAGTTCGTGTGCTTCCTCCTGCAGGAGGACCCCGCCAAGCGTCCCTCG
GCTGCGCTGGCCCTCCAGGAGCAGTGGCTGCAGGCCGGCAACGGCAGAAGCACGGGCGTC
CTCGACACGTCCAGACTGACTTCCTTCATTGAGCGGCGCAAACACCAGAATGATGTTCGA
CCTATCCGTAGCATTAAAAACTTTCTGCAGAGCAGGCTTCTGCCTAGAGTTTTG
Protein sequence predicted based on nucleotide sequence
MSGSSGGAAAPAASSGPAAAASAAGSGCGGGAGEGAEEAAKDLADIAAFFRSGFRKNDEM KAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVC KRGFTVIVDMRGSKWDSIKPLLKILQESFPCCIHVALIIKPDNFWQKQRTNFGSSKFEFE TNMVSLEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNATHMLSRLEELQDI LAKKELPQDLEGARNMIEEHSQLKKKVIKAPIEDLDLEGQKLLQRIQSSESFPKKNSGSG NADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMFDWITHNK GLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQIR QIASQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKACGEVDLPSELQDL EDAIHHHQGIYEHITLAYSEVSQDGKSLLDKLQRPLTPGSSDSLTASANYSKAVHHVLDV IHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLSKHTGVGKSL HRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRIQDFV RRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDDVYAESVEAVQDLIKRFGQQQQTT LQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKI KLELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNN LTFDVIHQGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHR KHLEQCVQLRHLQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKT HQSALQVQQKAEAMLQANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTS EQVCSVLESLEQEYKREEDWCGGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRN ADVFLKYLHRNSVNMPGMVTHIKAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQY VVFERSAKQALEWIHDNGEFYLSTHTSTGSSIQHTQELLKEHEEFQITAKQTKERVKLLI QLADGFCEKGHAHAAEIKKCVTAVDKRYRDFSLRMEKYRTSLEKALGISSDSNKSSKSLQ LDIIPASIPGSEVKLRDAAHELNEEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLW EMTSGVEEIPPGIVNKELIIFGNMQEIYEFHNNIFLKELEKYEQLPEDVGHCFVTWADKF QMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANSISSYLIKPVQRITKYQLLLKELL TCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIESQGELILQESFQVWDPKTL IRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEHVEGDPCKFALWVG RTPTSDNKIVLKASSIENKQDWIKHIREVIQERTIHLKGALKEPIHIPKTAPATRQKGRR DGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACNSNELTIRRG QTVEVLERPHDKPDWCLVRTTDRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDSLSVS SNDASPPASVASLQPHMIGAQSSPGPKRPGNTLRKWLTSPVRRLSSGKADGHVKKLAHKH KKSREVRKSADAGSQKDSDDSAATPQDETVEERGRNEGLSSGTLSKSSSSGMQSCGEEEG EEGADAVPLPPPMAIQQHSLLQPDSQDDKASSRLLVRPTSSETPSAAELVSAIEELVKSK MALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEMEERKSSSLKRRHYVLQELVET ERDYVRDLGYVVEGYMALMKEDGVPDDMKGKDKIVFGNIHQIYDWHRDFFLGELEKCLED PEKLGSLFVKHERRLHMYIAYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLIKP VQRIMKYQLLLKDFLKYSKKASLDTSELERAVEVMCIVPRRCNDMMNVGRLQGFDGKIVA QGKLLLQDTFLVTDQDAGLLPRCRERRIFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKV SCLCLEENVENDPCKFALTSRTGDVVETFILHSSSPSVRQTWIHEINQILENQRNFLNAL TSPIEYQRNHSGGGGGGGSGGSGGGGGSGGGGAPSGGSGHSGGPSSCGGAPSTSRSRPSR IPQPVRHHPPVLVSSAASSQAEADKMSGTSTPGPSLPPPGAAPEAGPSAPSRRPPGADAE GSEREAEPIPKMKVLESPRKGAANASGSSPDAPAKDARASLGTLPLGKPRAGAASPLNSP LSSAVPSLGKEPFPPSSPLQKGGSFWSSIPASPASRPGSFTFPGDSDSLQRQTPRHAAPG KDTDRMSTCSSASEQSVQSTQSNGSESSSSSNISTMLVTHDYTAVKEDEINVYQGEVVQI LASNQQNMFLVFRAATDQCPAAEGWIPGFVLGHTSAVIVENPDGTLKKSTSWHTALRLRK KSEKKDKDGKREGKLENGYRKSREGLSNKVSVKLLNPNYIYDVPPEFVIPLSEVTCETGE TVVLRCRVCGRPKASITWKGPEHNTLNNDGHYSISYSDLGEATLKIVGVTTEDDGIYTCI AVNDMGSASSSASLRVLGPGMDGIMVTWKDNFDSFYSEVAELGRGRFSVVKKCDQKGTKR AVATKFVNKKLMKRDQVTHELGILQSLQHPLLVGLLDTFETPTSYILVLEMADQGRLLDC VVRWGSLTEGKIRAHLGEVLEAVRYLHNCRIAHLDLKPENILVDESLAKPTIKLADFGDA VQLNTTYYIHQLLGNPEFAAPEIILGNPVSLTSDTWSVGVLTYVLLSGVSPFLDDSVEET CLNICRLDFSFPDDYFKGVSQKAKEFVCFLLQEDPAKRPSAALALQEQWLQAGNGRSTGV LDTSRLTSFIERRKHQNDVRPIRSIKNFLQSRLLPRVL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_nHalo_DC(r4)
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp):
6140
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Arizona State University
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Joshua LaBaer
Ji Qiu
Justin Saul
Fernanda Festa
Andrea Throop
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3425
4107
death cassette
ccdB
ccdB death cassette (removed in plasmids containing a gene insert)
2785
2994
enhancer
CITE
CITE enhancer
17
515
protease cleavage site
TEV
TEV protease cleavage site
1422
1442
recombination site
attR1
attR1 recombination site
1458
1474
recombination site
attR2
recombination site
3145
3160
selectable marker
AmpR
ampicillin resistance
4205
4864
selectable marker
CmR
chloramphenicol resistance (removed in clones containing a gene insert)
1691
2347
ssDNA origin
f1
f1 origin
5633
5939
tag
HALO
N-terminal HALO tag
522
1409
