Gene:
Gene Symbol:
CENPF
Gene Name:
centromere protein F, 350/400kDa
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00946262
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 9345nts
Open reading frame: 1 to 9345
ATGAGCTGGGCTTTGGAAGAATGGAAAGAAGGGCTGCCTACAAGAGCTCTTCAGAAAATT
CAAGAGCTTGAAGGACAGCTTGACAAACTGAAGAAGGAAAAGCAGCAAAGGCAGTTTCAG
CTTGACAGTCTCGAGGCTGCGCTGCAGAAGCAAAAACAGAAGGTTGAAAATGAAAAAACC
GAGGGTACAAACCTGAAAAGGGAGAATCAAAGATTGATGGAAATATGTGAAAGTCTGGAG
AAAACTAAGCAGAAGATTTCTCATGAACTTCAAGTCAAGGAGTCACAAGTGAATTTCCAG
GAAGGACAACTGAATTCAGGCAAAAAACAAATAGAAAAACTGGAACAGGAACTTAAAAGG
TGTAAATCTGAGCTTGAAAGAAGCCAACAAGCTGCGCAGTCTGCAGATGTCTCTCTGAAT
CCATGCAATACACCACAAAAAATTTTTACAACTCCACTAACACCAAGTCAATATTATAGT
GGTTCCAAGTATGAAGATCTAAAAGAAAAATATAATAAAGAGGTTGAAGAACGAAAAAGA
TTAGAGGCAGAGGTTAAAGCCTTGCAGGCTAAAAAAGCAAGCCAGACTCTTCCACAAGCC
ACCATGAATCACCGCGACATTGCCCGGCATCAGGCTTCATCATCTGTGTTCTCATGGCAG
CAAGAGAAGACCCCAAGTCATCTTTCATCTAATTCTCAAAGAACTCCAATTAGGAGAGAT
TTCTCTGCATCTTACTTTTCTGGGGAACAAGAGGTGACTCCAAGTCGATCAACTTTGCAA
ATAGGGAAAAGAGATGCTAATAGCAGTTTCTTTGACAATTCTAGCAGTCCTCATCTTTTG
GATCAATTAAAAGCGCAGAATCAAGAGCTAAGAAACAAGATTAATGAGTTGGAACTACGC
CTGCAAGGACATGAAAAAGAAATGAAAGGCCAAGTGAATAAGTTTCAAGAACTCCAACTC
CAACTGGAGAAAGCAAAAGTGGAATTAATTGAAAAAGAGAAAGTTTTGAACAAATGTAGG
GATGAACTAGTGAGAACAACAGCACAATACGACCAGGCGTCAACCAAGTATACTGCATTG
GAACAAAAACTGAAAAAATTGACGGAAGATTTGAGTTGTCAGCGACAAAATGCAGAAAGT
GCCAGATGTTCTCTGGAACAGAAAATTAAGGAAAAAGAAAAGGAGTTTCAAGAGGAGCTC
TCCCGTCAACAGCGTTCTTTCCAAACACTGGACCAGGAGTGCATCCAGATGAAGGCCAGA
CTCACCCAGGAGTTACAGCAAGCCAAGAATATGCACAACGTCCTGCAGGCTGAACTGGAT
AAACTCACATCAGTAAAGCAACAGCTAGAAAACAATTTGGAAGAGTTTAAGCAAAAGTTG
TGCAGAGCTGAACAGGCGTTCCAGGCGAGTCAGATCAAGGAGAATGAGCTGAGGAGAAGC
ATGGAGGAAATGAAGAAGGAAAACAACCTCCTTAAGAGTCACTCTGAGCAAAAGGCCAGA
GAAGTCTGCCACCTGGAGGCAGAACTCAAGAACATCAAACAGTGTTTAAATCAGAGCCAG
AATTTTGCAGAAGAAATGAAAGCGAAGAATACCTCTCAGGAAACCATGTTAAGAGATCTT
CAAGAAAAAATAAATCAGCAAGAAAACTCCTTGACTTTAGAAAAACTGAAGCTTGCTGTG
GCTGATCTGGAAAAGCAGCGAGATTGTTCTCAAGACCTTTTGAAGAAAAGAGAACATCAC
ATTGAACAACTTAATGATAAGTTAAGCAAGACAGAGAAAGAGTCCAAAGCCTTGCTGAGT
GCTTTAGAGTTAAAAAAGAAAGAATATGAAGAATTGAAAGAAGAGAAAACTCTGTTTTCT
TGTTGGAAAAGTGAAAACGAAAAACTTTTAACTCAGATGGAATCAGAAAAGGAAAACTTG
CAGAGTAAAATTAATCACTTGGAAACTTGTCTGAAGACACAGCAAATAAAAAGTCATGAA
TACAACGAGAGAGTAAGAACGCTGGAGATGGACAGAGAAAACCTAAGTGTCGAGATCAGA
AACCTTCACAACGTGTTAGACAGTAAGTCAGTGGAGGTAGAGACCCAGAAACTAGCTTAT
ATGGAGCTACAGCAGAAAGCTGAGTTCTCAGATCAGAAACATCAGAAGGAAATAGAAAAT
ATGTGTTTGAAGACTTCTCAGCTTACTGGGCAAGTTGAAGATCTAGAACACAAGCTTCAG
TTACTGTCAAATGAAATAATGGACAAAGACCGGTGTTACCAAGACTTGCATGCCGAATAT
GAGAGCCTCAGGGATCTGCTAAAATCCAAAGATGCTTCTCTGGTGACAAATGAAGATCAT
CAGAGAAGTCTTTTGGCTTTTGATCAGCAGCCTGCCATGCATCATTCCTTTGCAAATATA
ATTGGAGAACAAGGAAGCATGCCTTCAGAGAGGAGTGAATGTCGTTTAGAAGCAGACCAA
AGTCCGAAAAATTCTGCCATCCTACAAAATAGAGTTGATTCACTTGAATTTTCATTAGAG
TCTCAAAAACAGATGAACTCAGACCTGCAAAAGCAGTGTGAAGAGTTGGTGCAAATCAAA
GGAGAAATAGAAGAAAATCTCATGAAAGCAGAACAGATGCATCAAAGTTTTGTGGCTGAA
ACAAGTCAGCGCATTAGTAAGTTACAGGAAGACACTTCTGCTCACCAGAATGTTGTTGCT
GAAACCTTAAGTGCCCTTGAGAACAAGGAAAAAGAGCTGCAACTTTTAAATGATAAGGTA
GAAACTGAGCAGGCAGAGATTCAAGAATTAAAAAAGAGCAACCATCTACTTGAAGACTCT
CTAAAGGAGCTACAACTTTTATCCGAAACCCTAAGCTTGGAGAAGAAAGAAATGAGTTCC
ATCATTTCTCTAAATAAAAGGGAAATTGAAGAGCTGACCCAAGAGAATGGGACTCTTAAG
GAAATTAATGCATCCTTAAATCAAGAGAAGATGAACTTAATCCAGAAAAGTGAGAGTTTT
GCAAACTATATAGATGAAAGGGAGAAAAGCATTTCAGAGTTATCTGATCAGTACAAGCAA
GAAAAACTTATTTTACTACAAAGATGTGAAGAAACCGGAAATGCATATGAGGATCTTAGT
CAAAAATACAAAGCAGCACAGGAAAAGAATTCTAAATTAGAATGCTTGCTAAATGAATGC
ACTAGTCTTTGTGAAAATAGGAAAAATGAGTTGGAACAGCTAAAGGAAGCATTTGCAAAG
GAACACCAAGAATTCTTAACAAAATTAGCATTTGCTGAAGAAAGAAATCAGAATCTGATG
CTAGAGTTGGAGACAGTGCAGCAAGCTCTGAGATCTGAGATGACAGATAACCAAAACAAT
TCTAAGAGCGAGGCTGGTGGTTTAAAGCAAGAAATCATGACTTTAAAGGAAGAACAAAAC
AAAATGCAAAAGGAAGTTAATGACTTATTACAAGAGAATGAACAGCTGATGAAGGTAATG
AAGACTAAACATGAATGTCAAAATCTAGAATCAGAACCAATTAGGAACTCTGTGAAAGAA
AGAGAGAGTGAGAGAAATCAATGTAATTTTAAACCTCAGATGGATCTTGAAGTTAAAGAA
ATTTCTCTAGATAGTTATAATGCGCAGTTGGTGCAATTAGAAGCTATGCTAAGAAATAAG
GAATTAAAACTTCAGGAAAGTGAGAAGGAGAAGGAGTGCCTGCAGCATGAATTACAGACA
ATTAGAGGAGATCTTGAAACCAGCAATTTGCAAGACATGCAGTCACAAGAAATTAGTGGC
CTTAAAGACTGTGAAATAGATGCGGAAGAAAAGTATATTTCAGGGCCTCATGAGTTGTCA
ACAAGTCAAAACGACAATGCACACCTTCAGTGCTCTCTGCAAACAACAATGAACAAGCTG
AATGAGCTAGAGAAAATATGTGAAATACTGCAGGCTGAAAAGTATGAACTCGTAACTGAG
CTGAATGATTCAAGGTCAGAATGTATCACAGCAACTAGGAAAATGGCAGAAGAGGTAGGG
AAACTACTAAATGAAGTTAAAATATTAAATGATGACAGTGGTCTTCTCCATGGTGAGTTA
GTGGAAGACATACCAGGAGGTGAATTTGGTGAACAACCAAATGAACAGCACCCTGTGTCT
TTGGCTCCATTGGACGAGAGTAATTCCTACGAGCACTTGACATTGTCAGACAAAGAAGTT
CAAATGCACTTTGCCGAATTGCAAGAGAAATTCTTATCTTTACAAAGTGAACACAAAATT
TTACATGATCAGCACTGTCAGATGAGCTCTAAAATGTCAGAGCTGCAGACCTATGTTGAC
TCATTAAAGGCCGAAAATTTGGTCTTGTCAACGAATCTGAGAAACTTTCAAGGTGACTTG
GTGAAGGAGATGCAGCTGGGCTTGGAGGAGGGGCTCGTTCCATCCCTGTCATCCTCTTGT
GTGCCTGACAGCTCTAGTCTTAGCAGTTTGGGAGACTCCTCCTTTTACAGAGCTCTTTTA
GAACAGACAGGAGATATGTCTCTTTTGAGTAATTTAGAAGGGGCTGTTTCAGCAAACCAG
TGCAGTGTAGATGAAGTATTTTGCAGCAGTCTGCAGGAGGAGAATCTGACCAGGAAAGAA
ACCCCTTCGGCCCCAGCGAAGGGTGTTGAAGAGCTTGAGTCCCTCTGTGAGGTGTACCGG
CAGTCCCTCGAGAAGCTAGAAGAGAAAATGGAAAGTCAAGGGATTATGAAAAATAAGGAA
ATTCAAGAGCTCGAGCAGTTATTAAGTTCTGAAAGGCAAGAGCTTGACTGCCTTAGGAAG
CAGTATTTGTCAGAAAATGAACAGTGGCAACAGAAGCTGACAAGCGTGACTCTGGAGATG
GAGTCCAAGTTGGCGGCAGAAAAGAAACAGACGGAACAACTGTCACTTGAGCTGGAAGTA
GCACGACTCCAGCTACAAGGTCTGGACTTAAGTTCTCGGTCTTTGCTTGGCATCGACACA
GAAGATGCTATTCAAGGCCGAAATGAGAGCTGTGACATATCAAAAGAACATACTTCAGAA
ACTACAGAAAGAACACCAAAGCATGATGTTCATCAGATTTGTGATAAAGATGCTCAGCAG
GACCTCAATCTAGACATTGAGAAAATAACTGAGACTGGTGCAGTGAAACCCACAGGAGAG
TGCTCTGGGGAACAGTCCCCAGATACCAATTATGAGCCTCCAGGGGAAGATAAAACCCAG
GGCTCTTCAGAATGCATTTCTGAATTGTCATTTTCTGGTCCTAATGCTTTGGTACCTATG
GATTTCCTGGGGAATCAGGAAGATATCCATAATCTTCAACTGCGGGTAAAAGAGACATCA
AATGAGAATTTGAGATTACTTCATGTGATAGAGGACCGTGACAGAAAAGTTGAAAGTTTG
CTAAATGAAATGAAAGAATTAGACTCAAAACTCCATTTACAGGAGGTACAACTAATGACC
AAAATTGAAGCATGCATAGAATTGGAAAAAATAGTTGGGGAACTTAAGAAAGAAAACTCA
GATTTAAGTGAAAAATTGGAATATTTTTCTTGTGATCACCAGGAGTTACTCCAGAGAGTA
GAAACTTCTGAAGGCCTCAATTCTGATTTAGAAATGCATGCAGATAAATCATCACGTGAA
GATATTGGAGATAATGTGGCCAAGGTGAATGACAGCTGGAAGGAGAGATTTCTTGATGTG
GAAAATGAGCTGAGTAGGATCAGATCGGAGAAAGCTAGCATTGAGCATGAAGCCCTCTAC
CTGGAGGCTGACTTAGAGGTAGTTCAAACAGAGAAGCTATGTTTAGAAAAAGACAATGAA
AATAAGCAGAAGGTTATTGTCTGCCTTGAAGAAGAACTCTCAGTGGTCACAAGTGAGAGA
AACCAGCTTCGTGGAGAATTAGATACTATGTCAAAAAAAACCACGGCACTGGATCAGTTG
TCTGAAAAAATGAAGGAGAAAACACAAGAGCTTGAGTCTCATCAAAGTGAGTGTCTCCAT
TGCATTCAGGTGGCAGAGGCAGAGGTGAAGGAAAAGACGGAACTCCTTCAGACTTTGTCC
TCTGATGTGAGTGAGCTGTTAAAAGACAAAACTCATCTCCAGGAAAAGCTGCAGAGTTTG
GAAAAGGACTCACAGGCACTGTCTTTGACAAAATGTGAGCTGGAAAACCAAATTGCACAA
CTGAATAAAGAGAAAGAATTGCTTGTCAAGGAATCTGAAAGCCTGCAGGCCAGACTGAGT
GAATCAGATTATGAAAAGCTGAATGTCTCCAAGGCCTTGGAGGCCGCACTGGTGGAGAAA
GGTGAGTTCGCATTGAGGCTGAGCTCAACACAGGAGGAAGTGCATCAGCTGAGAAGAGGC
ATCGAGAAACTGAGAGTTCGCATTGAGGCCGATGAAAAGAAGCAGCTGCACATCGCAGAG
AAACTGAAAGAACGCGAGCGGGAGAATGATTCACTTAAGGATAAAGTTGAGAACCTTGAA
AGGGAATTGCAGATGTCAGAAGAAAACCAGGAGCTAGTGATTCTTGATGCCGAGAATTCC
AAAGCAGAAGTAGAGACTCTAAAAACACAAATAGAAGAGATGGCCAGAAGCCTGAAAGTT
TTTGAATTAGACCTTGTCACGTTAAGGTCTGAAAAAGAAAATCTGACAAAACAAATACAA
GAAAAACAAGGTCAGTTGTCAGAACTAGACAAGTTACTCTCTTCATTTAAAAGTCTGTTA
GAAGAAAAGGAGCAAGCAGAGATACAGATCAAAGAAGAATCTAAAACTGCAGTGGAGATG
CTTCAGAATCAGTTAAAGGAGCTAAATGAGGCAGTAGCAGCCTTGTGTGGTGACCAAGAA
ATTATGAAGGCCACAGAACAGAGTCTAGACCCACCAATAGAGGAAGAGCATCAGCTGAGA
AATAGCATTGAAAAGCTGAGAGCCCGCCTAGAAGCTGATGAAAAGAAGCAGCTCTGTGTC
TTACAACAACTGAAGGAAAGTGAGCATCATGCAGATTTACTTAAGGGTAGAGTGGAGAAC
CTTGAAAGAGAGCTAGAGATAGCCAGGACAAACCAAGAGCATGCAGCTCTTGAGGCAGAG
AATTCCAAAGGAGAGGTAGAGACCCTAAAAGCAAAAATAGAAGGGATGACCCAAAGTCTG
AGAGGTCTGGAATTAGATGTTGTTACTATAAGGTCAGAAAAAGAAAATCTGACAAATGAA
TTACAAAAAGAGCAAGAGCGAATATCTGAATTAGAAATAATAAATTCATCATTTGAAAAT
ATTTTGCAAGAAAAAGAGCAAGAGAAAGTACAGATGAAAGAAAAATCAAGCACTGCCATG
GAGATGCTTCAAACACAATTAAAAGAGCTCAATGAGAGAGTGGCAGCCCTGCATAATGAC
CAAGAAGCCTGTAAGGCCAAAGAGCAGAATCTTAGTAGTCAAGTAGAGTGTCTTGAACTT
GAGAAGGCTCAGTTGCTACAAGGCCTTGATGAGGCCAAAAATAATTATATTGTTTTGCAA
TCTTCAGTGAATGGCCTCATTCAAGAAGTAGAAGATGGCAAGCAGAAACTGGAGAAGAAG
GATGAAGAAATCAGTAGACTGAAAAATCAAATTCAAGACCAAGAGCAGCTTGTCTCTAAA
CTGTCCCAGGTGGAAGGAGAGCACCAACTTTGGAAGGAGCAAAACTTAGAACTGAGAAAT
CTGACAGTGGAATTGGAGCAGAAGATCCAAGTGCTACAATCCAAAAATGCCTCTTTGCAG
GACACATTAGAAGTGCTGCAGAGTTCTTACAAGAATCTAGAGAATGAGCTTGAATTGACA
AAAATGGACAAAATGTCCTTTGTTGAAAAAGTAAACAAAATGACTGCAAAGGAAACTGAG
CTGCAGAGGGAAATGCATGAGATGGCACAGAAAACAGCAGAGCTGCAAGAAGAACTCAGT
GGAGAGAAAAATAGGCTAGCTGGAGAGTTGCAGTTACTGTTGGAAGAAATAAAGAGCAGC
AAAGATCAATTGAAGGAGCTCACACTAGAAAATAGTGAATTGAAGAAGAGCCTAGATTGC
ATGCACAAAGACCAGGTGGAAAAGGAAGGGAAAGTGAGAGAGGAAATAGCTGAATATCAG
CTACGGCTTCATGAAGCTGAAAAGAAACACCAGGCTTTGCTTTTGGACACAAACAAACAG
TATGAAGTAGAAATCCAGACATACCGAGAGAAATTGACTTCTAAAGAAGAATGTCTCAGT
TCACAGAAGCTGGAGATAGACCTTTTAAAGTCTAGTAAAGAAGAGCTCAATAATTCATTG
AAAGCTACTACTCAGATTTTGGAAGAATTGAAGAAAACCAAGATGGACAATCTAAAATAT
GTAAATCAGTTGAAGAAGGAAAATGAACGTGCCCAGGGGAAAATGAAGTTGTTGATCAAA
TCCTGTAAACAGCTGGAAGAGGAAAAGGAGATACTGCAGAAAGAACTCTCTCAACTTCAA
GCTGCACAGGAGAAGCAGAAAACAGGTACTGTTATGGATACCAAGGTCGATGAATTAACA
ACTGAGATCAAAGAACTGAAAGAAACTCTTGAAGAAAAAACCAAGGAGGCAGATGAATAC
TTGGATAAGTACTGTTCCTTGCTTATAAGCCATGAAAAGTTAGAGAAAGCTAAAGAGATG
TTAGAGACACAAGTGGCCCATCTGTGTTCACAGCAATCTAAACAAGATTCCCGAGGGTCT
CCTTTGCTAGGTCCAGTTGTTCCAGGACCATCTCCAATCCCTTCTGTTACTGAAAAGAGG
TTATCATCTGGCCAAAATAAAGCTTCAGGCAAGAGGCAAAGATCCAGTGGAATATGGGAG
AATGGTAGAGGACCAACACCTGCTACCCCAGAGAGCTTTTCTAAAAAAAGCAAGAAAGCA
GTCATGAGTGGTATTCACCCTGCAGAAGACACGGAAGGTACTGAGTTTGAGCCAGAGGGA
CTTCCAGAAGTTGTAAAGAAAGGGTTTGCTGACATCCCGACAGGAAAGACTAGCCCATAT
ATCCTGCGAAGAACAACCATGGCAACTCGGACCAGCCCCCGCCTGGCTGCACAGAAGTTA
GCGCTATCCCCACTGAGTCTCGGCAAAGAAAATCTTGCAGAGTCCTCCAAACCAACAGCT
GGTGGCAGCAGATCACAAAAGGTCAAAGTTGCTCAGCGGAGCCCAGTAGATTCAGGCACC
ATCCTCCGAGAACCCACCACGAAATCCGTCCCAGTCAATAATCTTCCTGAGAGAAGTCCG
ACTGACAGCCCCAGAGAGGGCCTGAGGGTCAAGCGAGGCCGACTTGTCCCCAGCCCCAAA
GCTGGACTGGAGTCCAACGGCAGTGAGAACTGTAAGGTCCAGTTG
Protein sequence predicted based on nucleotide sequence
MSWALEEWKEGLPTRALQKIQELEGQLDKLKKEKQQRQFQLDSLEAALQKQKQKVENEKT EGTNLKRENQRLMEICESLEKTKQKISHELQVKESQVNFQEGQLNSGKKQIEKLEQELKR CKSELERSQQAAQSADVSLNPCNTPQKIFTTPLTPSQYYSGSKYEDLKEKYNKEVEERKR LEAEVKALQAKKASQTLPQATMNHRDIARHQASSSVFSWQQEKTPSHLSSNSQRTPIRRD FSASYFSGEQEVTPSRSTLQIGKRDANSSFFDNSSSPHLLDQLKAQNQELRNKINELELR LQGHEKEMKGQVNKFQELQLQLEKAKVELIEKEKVLNKCRDELVRTTAQYDQASTKYTAL EQKLKKLTEDLSCQRQNAESARCSLEQKIKEKEKEFQEELSRQQRSFQTLDQECIQMKAR LTQELQQAKNMHNVLQAELDKLTSVKQQLENNLEEFKQKLCRAEQAFQASQIKENELRRS MEEMKKENNLLKSHSEQKAREVCHLEAELKNIKQCLNQSQNFAEEMKAKNTSQETMLRDL QEKINQQENSLTLEKLKLAVADLEKQRDCSQDLLKKREHHIEQLNDKLSKTEKESKALLS ALELKKKEYEELKEEKTLFSCWKSENEKLLTQMESEKENLQSKINHLETCLKTQQIKSHE YNERVRTLEMDRENLSVEIRNLHNVLDSKSVEVETQKLAYMELQQKAEFSDQKHQKEIEN MCLKTSQLTGQVEDLEHKLQLLSNEIMDKDRCYQDLHAEYESLRDLLKSKDASLVTNEDH QRSLLAFDQQPAMHHSFANIIGEQGSMPSERSECRLEADQSPKNSAILQNRVDSLEFSLE SQKQMNSDLQKQCEELVQIKGEIEENLMKAEQMHQSFVAETSQRISKLQEDTSAHQNVVA ETLSALENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLSLEKKEMSS IISLNKREIEELTQENGTLKEINASLNQEKMNLIQKSESFANYIDEREKSISELSDQYKQ EKLILLQRCEETGNAYEDLSQKYKAAQEKNSKLECLLNECTSLCENRKNELEQLKEAFAK EHQEFLTKLAFAEERNQNLMLELETVQQALRSEMTDNQNNSKSEAGGLKQEIMTLKEEQN KMQKEVNDLLQENEQLMKVMKTKHECQNLESEPIRNSVKERESERNQCNFKPQMDLEVKE ISLDSYNAQLVQLEAMLRNKELKLQESEKEKECLQHELQTIRGDLETSNLQDMQSQEISG LKDCEIDAEEKYISGPHELSTSQNDNAHLQCSLQTTMNKLNELEKICEILQAEKYELVTE LNDSRSECITATRKMAEEVGKLLNEVKILNDDSGLLHGELVEDIPGGEFGEQPNEQHPVS LAPLDESNSYEHLTLSDKEVQMHFAELQEKFLSLQSEHKILHDQHCQMSSKMSELQTYVD SLKAENLVLSTNLRNFQGDLVKEMQLGLEEGLVPSLSSSCVPDSSSLSSLGDSSFYRALL EQTGDMSLLSNLEGAVSANQCSVDEVFCSSLQEENLTRKETPSAPAKGVEELESLCEVYR QSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEM ESKLAAEKKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTSE TTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSPDTNYEPPGEDKTQ GSSECISELSFSGPNALVPMDFLGNQEDIHNLQLRVKETSNENLRLLHVIEDRDRKVESL LNEMKELDSKLHLQEVQLMTKIEACIELEKIVGELKKENSDLSEKLEYFSCDHQELLQRV ETSEGLNSDLEMHADKSSREDIGDNVAKVNDSWKERFLDVENELSRIRSEKASIEHEALY LEADLEVVQTEKLCLEKDNENKQKVIVCLEEELSVVTSERNQLRGELDTMSKKTTALDQL SEKMKEKTQELESHQSECLHCIQVAEAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSL EKDSQALSLTKCELENQIAQLNKEKELLVKESESLQARLSESDYEKLNVSKALEAALVEK GEFALRLSSTQEEVHQLRRGIEKLRVRIEADEKKQLHIAEKLKERERENDSLKDKVENLE RELQMSEENQELVILDAENSKAEVETLKTQIEEMARSLKVFELDLVTLRSEKENLTKQIQ EKQGQLSELDKLLSSFKSLLEEKEQAEIQIKEESKTAVEMLQNQLKELNEAVAALCGDQE IMKATEQSLDPPIEEEHQLRNSIEKLRARLEADEKKQLCVLQQLKESEHHADLLKGRVEN LERELEIARTNQEHAALEAENSKGEVETLKAKIEGMTQSLRGLELDVVTIRSEKENLTNE LQKEQERISELEIINSSFENILQEKEQEKVQMKEKSSTAMEMLQTQLKELNERVAALHND QEACKAKEQNLSSQVECLELEKAQLLQGLDEAKNNYIVLQSSVNGLIQEVEDGKQKLEKK DEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQSKNASLQ DTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTAELQEELS GEKNRLAGELQLLLEEIKSSKDQLKELTLENSELKKSLDCMHKDQVEKEGKVREEIAEYQ LRLHEAEKKHQALLLDTNKQYEVEIQTYREKLTSKEECLSSQKLEIDLLKSSKEELNNSL KATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQKELSQLQ AAQEKQKTGTVMDTKVDELTTEIKELKETLEEKTKEADEYLDKYCSLLISHEKLEKAKEM LETQVAHLCSQQSKQDSRGSPLLGPVVPGPSPIPSVTEKRLSSGQNKASGKRQRSSGIWE NGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPY ILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGT ILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
