Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
No ATG. Codon optimized
Species:
Coxsackievirus A6
Type:
cDNA
Vector Name:
pLenti6.3/V5-DEST
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
CaCD00956018
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1386nts
Open reading frame: 1 to 1386

GGGGAGATTCAGTGGGTTAAGCCTAACAAGGAAACCGGGCGGCTGAACATCAATGGACCA
ACAAGAACTAAGCTGGAGCCGTCCGTCTTCCACGACATCTTTGAGGGAAACAAGGAACCC
GCCGTCCTGCATAGCAAAGACCCTCGGCTCGAGGTTGATTTCGAACAGGCCCTGTTTTCC
AAATACGTGGGCAATACTCTGCACGAGCCAGATGAATACATTAAGGAGGCCGCTCTGCAT
TATGCCAACCAGCTGAAGCAGCTGGACATCGATACATCACAGATGAGCATGGAGGAAGCT
TGCTATGGGACTGACAACCTGGAAGCAATTGATCTGCACACCAGTGCTGGTTACCCCTAT
TCAGCACTGGGCATTAAGAAAAGGGACATCCTGGATCCTACCACACGCGACGTGTCCAAG
ATGAAATTCTACATGGACAAGTATGGACTGGACCTGCCCTACACTACCTATGTCAAAGAC
GAGCTGCGATCAATTGATAAGATCAAGAAAGGCAAAAGCCGTCTCATCGAAGCAAGCTCC
CTGAATGATAGCGTGTACCTGAGAATGGCCTTCGGGCACCTGTATGAGACCTTTCATGCC
AACCCTGGCACCGTGACAGGGAGTGCTGTCGGATGTGACCCAGATATTTTCTGGTCAAAG
CTGCCCATCCTGCTCCCTGGGAGCCTCTTCGCATTTGACTACTCCGGATATGATGCCTCT
CTGAGTCCAGTGTGGTTTCGAGCCCTGGAGCTGGTGCTGCGTGAAATCGGTTACTCCGAC
GAGGCCGTGAGCCTGATTGAAGGCATCAATCACACCCACCACGTGTACCGAAACAAGACA
TATTGCGTGCTGGGCGGGATGCCCTCCGGATGTTCTGGCACCAGCATTTTCAATTCCATG
ATCAACAACATCATCATCCGCTCTCTGCTGATCAAGACCTTCAAGGGGATCGACCTGGAT
GAGCTGAACATGGTTGCCTACGGAGACGATGTGCTGGCTTCTTATCCCTTCCCTATTGAC
TGCAGTGAGCTGGCTAGAACCGGCAAGGAATACGGCCTGACTATGACCCCAGCAGATAAA
AGCCCGTGTTTTAACGAAGTGAATTGGGAAAACGCCACCTTCCTGAAGAGGGGCTTTCTG
CCCGACGAGCAGTTCCCTTTTCTGATCCACCCAACAATGCCGATGAAGGAGATTCATGAA
TCTATCAGGTGGACAAAAGACGCACGCAATACTCAGGATCACGTGAGGAGTCTGTGCCTG
CTCGCTTGGCATAACGGCAAGCAGGAGTACGAAAAATTCGTCTCAGCCATTCGCAGCGTT
CCAATCGGAAAGGCACTGGCCATCCCCAATTATGAGAACCTGCGGAGAAACTGGCTCGAA
CTGTTT

Protein sequence predicted based on nucleotide sequence

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDIFEGNKEPAVLHSKDPRLEVDFEQALFS
KYVGNTLHEPDEYIKEAALHYANQLKQLDIDTSQMSMEEACYGTDNLEAIDLHTSAGYPY
SALGIKKRDILDPTTRDVSKMKFYMDKYGLDLPYTTYVKDELRSIDKIKKGKSRLIEASS
LNDSVYLRMAFGHLYETFHANPGTVTGSAVGCDPDIFWSKLPILLPGSLFAFDYSGYDAS
LSPVWFRALELVLREIGYSDEAVSLIEGINHTHHVYRNKTYCVLGGMPSGCSGTSIFNSM
INNIIIRSLLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCSELARTGKEYGLTMTPADK
SPCFNEVNWENATFLKRGFLPDEQFPFLIHPTMPMKEIHESIRWTKDARNTQDHVRSLCL
LAWHNGKQEYEKFVSAIRSVPIGKALAIPNYENLRRNWLELF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable