Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Burkholderia pseudomallei K96243
Type:
cDNA
Vector Name:
pTwist30 KAN Uni9
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
BpCD00959240
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1278nts
Open reading frame: 106 to 1383
ATGAACTGGGCTGCACGCAGGATTGGCGGACGATTTCACTATGGCTGGCTTGCAGCAGCA
GTCGTTTTCCTTATCTTGCTTGCTGCAGCCGGGACAAGAGCTACACCTTCTGTACTCATG
GTACCCCTGGAACACGAATTTGGTTGGTCTCGAGCCAGCATTAGCCTGGCTATCTCCGTC
AATATTGCATTGTACGGACTTACGGGCCCTTTTGCAGCTGCCGCCATGCAACGGTTTGGG
GTACGTCCAACTGTACTGGCGGCTCTTGGTGTTATGGGCATGGGAGTTGCGCTGAGTTCT
ATGATGACCGCCACCTGGCAGATGGTCCTGGTGTGGGGTGTAATGGTGGGCGGGGCCACC
GGGGTTGCCGCACTGACACTCTCTGCTACATTTGTGACCAGGTGGTTTGTGGCTCGCCGA
GGACTTGTCATGGGTTTGCTTACAGCCAGCAGCGCTACCGGGCAGCTGGTCTTTCTCCCA
ATGCTGGCCGCTATCGCCCAACGACATGGATGGCGCCCTGTAGTACTTACAGTGGCAGTT
GCTGCTGCAATTGCAGTTCCCCTGGTGGCACTCCTTCTTCCTGAAAGGCCAAGCGATGTA
GGCCTCAGGCCCTATGGAGAACCAGCCAATGCTCCAAGAGCCGAAGATGGTGCCAAAAGG
AATCCCATTGCCGTGGCCTTTGGGATGCTGGCCACTGCCAGCAAAACAAGAGATTTCTGG
CTCTTGTTCTTTTCTTTCTTTATATGTGGGGCCAGTACAAATGGATATGTTGGGACACAC
CTGATCGCCATGTGTTCCGATTATGGCATGACCCAAGTTCAGGGAGCATCATTGCTGGCT
GCTATGGGAGTTTTCGATTTGATTGGTACAACTATGAGTGGGTGGTTGTCTGATAGATAT
GATTCTAGGGTCCTGCTCTTTTGGTATTATGGACTCCGGGGACTCTCATTGATATACCTT
CCCCACGCTTTTGGGATTGACTTCTTTGGACTGCCACTGTTTGCCGTTTTCTATGGCTTG
GATTGGATCGCCACTGTTCCACCAACAGTTCGATTGGCTACAGATGTCTATGGGAAAGAC
GCCGCACCGGTGGTATTTGGGTGGATTGTTGCCGGGCATCAATTGGGTGCTGCCTTTGCC
GCCTTGGGAGCAGGTATGTTGAGGGCCTCACTGGGGACATACACAGTCGCTTCCATGATC
TCCGGCGGTCTTTGTATTGTGGGAGCTCTTATTGTTCTTCGTATTAATAGGGGCACCGCT
CGTGTCTCAGCTAGTGCCGCCCTGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGA
AAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATT
ATT
Protein sequence predicted based on nucleotide sequence
PSVLMVPLEHEFGWSRASISLAISVNIALYGLTGPFAAAAMQRFGVRPTVLAALGVMGMG VALSSMMTATWQMVLVWGVMVGGATGVAALTLSATFVTRWFVARRGLVMGLLTASSATGQ LVFLPMLAAIAQRHGWRPVVLTVAVAAAIAVPLVALLLPERPSDVGLRPYGEPANAPRAE DGAKRNPIAVAFGMLATASKTRDFWLLFFSFFICGASTNGYVGTHLIAMCSDYGMTQVQG ASLLAAMGVFDLIGTTMSGWLSDRYDSRVLLFWYYGLRGLSLIYLPHAFGIDFFGLPLFA VFYGLDWIATVPPTVRLATDVYGKDAAPVVFGWIVAGHQLGAAFAALGAGMLRASLGTYT VASMISGGLCIVGALIVLRINRGTARVSASAALDPAFLYKVGIIRKHCLSICCNEQVTIS QNKIII
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
50ug/mL of Kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pTwist30 KAN Uni9
Synonyms:
''
Description:
with rrnB T1 T2 (transcription terminator) and Origin and Kan-R; 50ug/mL of Kanamycin resistance in bacterial;
Comments:
Backbone vector from Twist biosciences
Size (bp):
2165
Parent Vector:
None
Properties:
''
Author Name:
Twist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
Origin
Origin
null
245
833
selectable marker
Kan-R
null
969
1784
trxn termination sequence
rrnB T1 T2 (transcription terminator)
rrnB T1 Terminator
1922
1968
