Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Burkholderia pseudomallei K96243
Type:
cDNA
Vector Name:
pTwist 30 pMB1-Kan-UCS1.2
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
BpCD00959420
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1308nts
Open reading frame: 1 to 1308
ATGAACATCATCTGGGCGCGCTTTCTGGTGTCGCTCGTGCTGCTCGCGCTCGTGAGCGTG
ATCATCGGCGCAATCGCGGGCCCCGTGCCGGGTCTCGTCTTCGCGCTCGCGATGCTCGTC
GTGCAGGGTTTCGTCAGCACCTTCCACACGCAGCGCCTCTGGCGCCTGCTCGATGCGCCC
GTCTACGGCGAAGTGCCGAGCGCGCCGGGCATCTGGGGCGAGATCTACTATCGGCTGCAC
AAACTCGCGAAGCAGTGGCACGCGCAGGTGCGCCAGGTCGAGCAGCAGCACTCGCGTTTC
ATCCAGGCGATCCAGGCGTCGCCGAACGGCGTCGCGATGCTCGACGATCGCGATCAGATC
GAGTGGTGCAACGCGATCGCCGAGGTGCACTTCGGGCTCGACGCGAAGCGCGACCTGCGC
CAGCACGTCACGCATCTCGTGCGCCATCCGGATTTCGTCCGCTATCTGAACGGCCAGCAT
TACGACGAAACGCTCGTGATGCGCGGCATGGGCGGCAATCGGCAGAACGTGCTTGCCGTG
CAGGTGTTTCCGTACGGCGAGAACCGCAAGCTGCTGCTCACGCAGGATATCACCGAGCTC
GAGCGCACCGATGCGATGCGGCGCGACTTCGTCGCGAACGTGTCGCACGAACTGAAGACG
CCGCTTACCGTGCTGTCGGGCTTTCTCGAGACGATGCGCGAGCTGCCGCTGTCGGACGAC
GAGCGCGCGCGCTATCTCGAACTGATGGAGCAGCAGGCGTCGCGGATGCGGCACATCGTC
ACCGATCTGCTCGTGCTCGCGAAGCTCGAGGGCGAAAGCAAGCCGCCCGCCGATCGCGCG
GTCGACATGCGCACGGTGCTCGACCATCTGCGGGACGATGCGCAGACGCTATCGGGCGGC
CATCACGAGATCACGTTCAAGGTTGACGAGACGCTCGCGGTGACGGGCGCGCAGACCGAG
ATCTTCAGCGCGCTCGGCAACCTCGTGACGAATGCGATCCGCTATACGCCGGAGGGCGGG
ACGATCCGCGTCGAATGGCGGCGCGACGGCGCGCAGGCGGTGTTCTCGGTCACCGATAGC
GGGCTCGGCATTCCGGCCGCCGAACTGCCGCGGCTCACCGAGCGGTTCTATCGCGTCGAC
CGCAGCCGCTCGCGCGACACGGGCGGCACGGGGCTCGGGCTCGCGATCGTCAAGCATGTG
TTGCAACGGCACGATGCGCAACTGTCGATCCAGAGCGAGGAGGGGCGTGGCAGCACGTTT
ACCGCGCGTTTTCCCGCGCATCGGACGATCAGCCGGAGGCATGCGGCGTGA
Protein sequence predicted based on nucleotide sequence
MNIIWARFLVSLVLLALVSVIIGAIAGPVPGLVFALAMLVVQGFVSTFHTQRLWRLLDAP VYGEVPSAPGIWGEIYYRLHKLAKQWHAQVRQVEQQHSRFIQAIQASPNGVAMLDDRDQI EWCNAIAEVHFGLDAKRDLRQHVTHLVRHPDFVRYLNGQHYDETLVMRGMGGNRQNVLAV QVFPYGENRKLLLTQDITELERTDAMRRDFVANVSHELKTPLTVLSGFLETMRELPLSDD ERARYLELMEQQASRMRHIVTDLLVLAKLEGESKPPADRAVDMRTVLDHLRDDAQTLSGG HHEITFKVDETLAVTGAQTEIFSALGNLVTNAIRYTPEGGTIRVEWRRDGAQAVFSVTDS GLGIPAAELPRLTERFYRVDRSRSRDTGGTGLGLAIVKHVLQRHDAQLSIQSEEGRGSTF TARFPAHRTISRRHAA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
50ug/mL of Kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pTwist 30 pMB1-Kan-UCS1.2
Synonyms:
''
Description:
with Origin and Kan-R and rrnB T1 T2 (transcription terminator); 50ug/mL of Kanamycin resistance in bacterial;
Comments:
Backbone vector from Twist biosciences
Size (bp):
2165
Parent Vector:
None
Properties:
''
Author Name:
Twist
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
Origin
Origin
null
245
833
Terminator
rrnB T1 T2 (transcription terminator)
null
1922
1968
selectable marker
Kan-R
null
969
1784
