Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
ASU_CoV_008-B
Keyword:
"Split in to 2 ORFs, with 50 aa overlap"
Species:
Human coronavirus NL63
Type:
cDNA
Vector Name:
pJFT7_cHalo-3xFlag_DC
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HcCD00960345
Price:
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AVAILABLE
No restriction
Special MTA: HALO
Special MTA: HALO
Insert sequence: 2496nts
Open reading frame: 1 to 2496
ATGGTAGCTCCAGAAGTTGACTGGGTTGCTTTTTACGGTTTTGAAAAGGCAGCACTTTTT
GCTTCTTTGGATGTAAAGCCATATGGTTACCCTAATGATTTTGTTGGTGGTTTTAGAGTT
CTTGGGACCACCGACAATAATTGTTGGGTTAATGCAACTTGTATAATTTTACAGTATCTT
AAGCCTACTTTTAAATCTAAGGGTTTAAATGTTCTTTGGAACAAATTTGTTACAGGTGAT
GTTGGACCTTTTGTTAGTTTTATTTATTTTATAACTATGTCTTCAAAGGGTCAAAAGGGT
GATGCTGAAGAGGCATTATCTAAATTGTCAGAGTATTTGATTAGTGATTCTATTGTTACT
CTTGAACAATATTCAACTTGTGACATTTGTAAAAGTACTGTAGTTGAAGTTAAAAGTGCT
ATTGTCTGTGCTAGTGTGCTTAAAGATGGTTGTGATGTTGGTTTTTGTCCACACAGACAT
AAATTGCGTTCACGTGTTAAGTTTGTTAATGGACGTGTTGTTATTACCAATGTTGGTGAA
CCTATAATTTCACAACCTTCTAAGTTGCTTAATGGTATTGCTTATACAACATTTTCAGGT
TCTTTTGATAACGGTCACTATGTAGTTTATGATGCTGCTAATAATGCTGTCTATGATGGT
GCTCGTTTATTTTCTTCAGATTTGTCTACTTTAGCTGTTACAGCTATTGTTGTAGTAGGT
GGTTGTGTAACATCTAATGTTCCAACAATTGTTAGTGAGAAAATTTCTGTTATGGATAAA
CTTGATACTGGTGCACAAAAATTTTTCCAATTTGGTGATTTTGTTATGAATAACATTGTT
CTGTTTTTAACTTGGTTGCTTAGTATGTTTAGTCTTTTACGTACTTCTATTATGAAGCAT
GATATTAAAGTTATTGCCAAGGCTCCTAAACGTACAGGTGTTATTTTGACACGTAGTTTT
AAGTATAACATTAGATCTGCTTTGTTTGTTATAAAGCAGAAGTGGTGTGTTATTGTTACT
TTGTTTAAGTTCTTATTATTATTATATGCTATTTATGCACTTGTTTTTATGATTGTGCAA
TTTAGTCCTTTTAATAGTCTTTTATGTGGTGACATTGTAAGTGGTTATGAAAAATCCACT
TTTAATAAGGATATTTATTGTGGTAATTCTATGGTTTGTAAGATGTGTTTGTTCAGTTAT
CAAGAGTTTAATGATTTGGATCATACTAGTCTTGTTTGGAAGCACATTCGTGATCCTATA
TTAATCAGTTTACAACCATTTGTTATACTTGTTATTTTGTTAATTTTTGGTAATATGTAT
TTGCGTTTTGGACTTTTATATTTTGTTGCACAATTTATTAGTACTTTTGGTTCTTTCTTA
GGCTTTCATCAGAAACAGTGGTTTTTACATTTTGTGCCGTTTGATGTTTTATGTAATGAG
TTTTTAGCTACATTTATTGTCTGCAAAATCGTTTTATTTGTTAGACATATTATTGTTGGC
TGTAATAATGCTGACTGTGTAGCTTGTTCTAAAAGTGCTAGACTTAAACGTGTACCACTT
CAAACTATTATTAATGGTATGCATAAATCATTCTATGTTAATGCTAATGGTGGTACTTGT
TTCTGTAATAAACATAACTTCTTTTGTGTTAATTGTGATTCTTTTGGGCCTGGTAATACT
TTTATTAATGGTGATATTGCAAGAGAGCTTGGTAATGTTGTTAAAACAGCTGTTCAACCC
ACAGCTCCTGCATATGTTATTATTGATAAGGTAGATTTTGTTAATGGATTTTATCGTCTT
TATAGTGGTGACACTTTTTGGCGGTATGACTTTGACATTACTGAATCTAAGTATAGTTGT
AAAGAGGTTCTGAAGAATTGTAATGTTTTAGAAAATTTTATTGTTTACAATAATAGTGGT
AGTAACATTACACAGATTAAAAATGCTTGTGTTTATTTTTCTCAATTGTTGTGTGAACCT
ATAAAGTTGGTAAATTCAGAGTTGTTGTCAACTTTATCTGTTGATTTTAATGGTGTTTTG
CATAAGGCATATGTTGATGTTTTGTGTAATAGTTTTTTTAAGGAGTTAACTGCTAACATG
TCCATGGCTGAATGTAAAGCTACACTTGGTTTGACTGTTTCTGATGATGATTTTGTTTCA
GCTGTTGCCAATGCACATAGGTATGACGTTTTGCTTTCAGATTTGTCATTTAATAATTTT
TTTATTTCTTATGCTAAACCTGAAGATAAGTTGTCCGTTTATGACATTGCTTGTTGTATG
CGTGCCGGTTCTAAGGTTGTTAACCATAATGTTTTAATTAAAGAGTCAATACCTATTGTT
TGGGGTGTCAAGGACTTTAATACTCTTTCTCAAGAAGGTAAGAAGTACCTTGTTAAAACA
ACTAAAGCAAAGGGTTTGACTTTTTTATTAACTTTTAATGATAACCAAGCAATTACACAA
GTTCCTGCTACTAGTATAGTTGCAAAACAGGGTGCT
Protein sequence predicted based on nucleotide sequence
MVAPEVDWVAFYGFEKAALFASLDVKPYGYPNDFVGGFRVLGTTDNNCWVNATCIILQYL KPTFKSKGLNVLWNKFVTGDVGPFVSFIYFITMSSKGQKGDAEEALSKLSEYLISDSIVT LEQYSTCDICKSTVVEVKSAIVCASVLKDGCDVGFCPHRHKLRSRVKFVNGRVVITNVGE PIISQPSKLLNGIAYTTFSGSFDNGHYVVYDAANNAVYDGARLFSSDLSTLAVTAIVVVG GCVTSNVPTIVSEKISVMDKLDTGAQKFFQFGDFVMNNIVLFLTWLLSMFSLLRTSIMKH DIKVIAKAPKRTGVILTRSFKYNIRSALFVIKQKWCVIVTLFKFLLLLYAIYALVFMIVQ FSPFNSLLCGDIVSGYEKSTFNKDIYCGNSMVCKMCLFSYQEFNDLDHTSLVWKHIRDPI LISLQPFVILVILLIFGNMYLRFGLLYFVAQFISTFGSFLGFHQKQWFLHFVPFDVLCNE FLATFIVCKIVLFVRHIIVGCNNADCVACSKSARLKRVPLQTIINGMHKSFYVNANGGTC FCNKHNFFCVNCDSFGPGNTFINGDIARELGNVVKTAVQPTAPAYVIIDKVDFVNGFYRL YSGDTFWRYDFDITESKYSCKEVLKNCNVLENFIVYNNSGSNITQIKNACVYFSQLLCEP IKLVNSELLSTLSVDFNGVLHKAYVDVLCNSFFKELTANMSMAECKATLGLTVSDDDFVS AVANAHRYDVLLSDLSFNNFFISYAKPEDKLSVYDIACCMRAGSKVVNHNVLIKESIPIV WGVKDFNTLSQEGKKYLVKTTKAKGLTFLLTFNDNQAITQVPATSIVAKQGA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pJFT7_cHalo-3xFlag_DC
Synonyms:
''
Description:
In vitro expression vector with a T7 promoter and C-terminal TEV-cleavable HALO-3xFlag tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments:
None
Size (bp):
6274
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning
Author Name:
Arizona State University
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Joshua LaBaer
Justin Saul
Ji Qiu
Fernanda Festa
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3559
4241
bacterial origin
M13 Ori
M13 origin
5365
6090
death cassette
ccdB
Gateway ccdB death Cassette (DC) (removed in plasmids with inserts)
556
2210
death cassette 2
ccdB (BsrGI removed)
ccdB (BsrGI removed)
1765
2070
enhancer
CITE
CITE enhancer
388
408
primer
NAP150
NAP150F forward sequencing primer cccattgtatgggatctgatc (sequencing starts before the 3xFlag-HALO tag)
388
408
primer
reverse primer
cHaloseqR reverse sequencing primer CAACATCGACGTAGTGCAT (used for sequencing the gene insert)
2351
2369
promoter
T7
T7 promoter
6250
3
protease cleavage site
TEV
TEV protease cleavage site EDLYFQS (removes C-terminal HALO 3xFlag tag)
2258
2278
recombination site
attR1
attR1 recombination site
531
555
recombination site
attR2
attR2 recombination site
2211
2235
reporter gene
LacZ
LacZ alpha gene
6095
6163
selectable marker
CmR
Chloramphenicol resistance gene (removed in plasmids containing gene inserts)
764
1420
selectable marker
AmpR
ampicillin resistance gene
4339
4998
ssDNA origin
f1
f1 origin
5767
6073
tag
Flag
C-terminal 3xFlag tag
3179
3247
tag
HALO
C-terminal Halo tag
2294
3178
trxn termination sequence
T7 term
T7 transcription terminator
3319
3337
