Gene:
Gene Symbol:
CIMG_09750
Gene Name:
hypothetical protein
Original Clone ID:
None
Keyword:
Codon optimized by Homo sapiens
Species:
Coccidioides immitis RS
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Partial protein;XP_001240129:7114-8032
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
CiCD00970157
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2757nts
Open reading frame: 1 to 2757
TCCGAATACGTGATTACAGTGAACGTTGAGACGGCCAAGAACGACGAAAAAGTGGTACTG
CGGTACTGGTCAGATGCGATCCCTGATTTCGAGGCCAAGAAATACGCTAGTTCTATGGCG
GAAATCTTAACGAAATTCCTGGACCAGCCTCGCCAGAAGGTAGCAACTCGAGACCCCGCT
ATCAAACCAAGCCTAGAGATTAGCCCCTCGGAAGATCAGGAGAGAAGCGACTCACCAGCT
AAACAGGAGAGCGAAAGGTTCTACGAGCAGTCCGTGGCCGGCTCATCTGCCGATCTGACG
AAAGTGGTCGACAGCTGTGTTAGAGAGGTCATCCAGCAGATGCTGGAGAACGGAACACTG
AAGAGCAATCCTTCAGTGAACATGGCCGACCTGGTTGATCGGAAAATCTCAGAGATCTTG
GGCCAGAATCAAGCACTGATCAAGCCCGTGCCCGGCAGTTCCCACGGCACGTTCAGCCCC
TACCGGAAGAAGGCCGGCCTGGATCTCCCTTCCACCAGCTCCGACTCCTACGAGGTGCTG
GACTACAACCCGAAGGACGATCCTGACACACACAGCATGGCTGCCCAGATCAGACGTAGG
GGCAGATCCGCAGTGATCGAGAAGAAGCTGTTGTCTCTCTGGAGCTCTATGCTGGAGATG
GACGAGGATGCTATTGCTCCCGATGACAACTTTTTCGAGCTCGGCGGCGATTCTTTGACT
GCCATGAAACTGGTTGGTGCCGCCAGAGAGGACGGACTCGCCTTGACTGTGGCCGATGTA
TTCAGAAACCCTGTCTTTGAGGATATGGTCACCATGATCCGGGTGGCTAGTCTCATGACG
GCGTACGAGGACGGCGATGGTGATGGCGACGGAGATGCAAACTCCAACGGCGATCTGTCC
AGCTACCACCGAACAGTGCCTGTCCTCCGGAGCGCTGCCAAATCAGAACTGTATCAACGG
TTCAGCCTGGTTAAGGCCACGAATATCGACGCGTTTCTGCAATCCAATATCTGCCCCAAG
GTAGGAGTCTTCAAGGGCGGCATCGCTGATGTACTGCCTGTGACAGATTTCCAGGGTCTG
GCCATCACCGGCTCTCTGCTGGAATCTAAGTGGATGCTGAACTACTTCTTCTTCGACGGC
AATGGGCCACTGGATCTGAAGCAACTGAAGCTCTCGGCTTTCCGCTTGGTCAATAGTATC
GACGTGTTGAGAACTGTATTCTTACCTCACGGCGATAGATTCCTGCAGGTGGTGCTGAGA
AAGATGAGACCCGATTTTTTCGTTTATGAGACCGAATCTAACATGGACGAGTTTACCGCT
ATGCTGCAACAGCGTGATAGGGATCAGGGACCTAGACTGGGCGAGCCTTTTTTCAACTTC
ATAGTCGTGAAGAGAAAGGACAGTGATCATCACAGAATCATTATTAGAATTAGTCACGCC
CAGTACGACGGGGTGTGCCTGCCAAAGATCTTCCTGGCATTGCAATCTGCTTACCAGGAC
GAGCCTCTGCCTCCTATGAGCTCTTTCGCCAACTACGTGAGAAGTACAGCATCTACTATT
ACAAGCGATCATTACCAGCACTGGCGAGCCCTACTTAAGGGCAGCAGAATGACCGAGATT
GTCCGCCGACACGGCCCAAACTACAGACGTTCCGCTGGGGCAACTACACAGCTGAAACAG
ACAATCCTCCTGCCGCCAATCGCTCATGGCAGTATCACGACGGCAACAGTAGTAAAATCA
GCTTGGGCCTTGGTACTGGCGCAGCTGACCGGACGTTCAGATGTAGTTTTTGGCCACACC
ATTAGTGGCAGAAATGCCACCGTTCCCGGAGTGGAAAGCACAGTCGGTCCCTGCTTGAAC
ATCGTGCCTGTGAGAGTGAGATTCTGCGAGGGTTGGACGGCCCTTGACCTGTTACGGTTT
GTGCAGGATCAGCAGGTTTCCAATATGCCTTACGAGGCGCTGGGATTTCGGGAAATATCT
AAACACTGCACCGAGTGGCCTGACTGGACAAACTACACCACGGTGGTGCAACACCAGAAC
GTGTCCTACGGCAGCGAAATGGAACTGGGAGGCAACACCTACAAGATAGGCGGCGTGGGA
GCCGATGAAGATTTTAGCGACTTTTCGGTCATAAGCACGCCTAAGGGGAGCGACAGATGC
GAGATTTCTTTATCCTTTTCTCAGAACGGCGACATCACCCCTATTTTTGCCCAGAAGGTG
CTCAACATGCTCTGCAACATCGCCTCTAATTTCACAGCTGAGCCTAACTCTGCTCTGATG
AGCCCCACCGAGCTGGGCGAGCTACCTCCTCAGGTGATCGATGAGACGGGTAGACAAAAC
GACAATTACTTCGTTTCCTCCCAGCTGCAGGGGCTGTCTCGGGCCGATCTGCTGGTACTC
TCGGATGTGCTTTCCCGATCCTGGCGCCAGGTGCTAGGGGATGATAACACGAACGGGCTG
AACTTGGAGTCTTCATTCTTCGACCTCAATGGCGACATTATGGGCCTCGCACAAGTTTCC
TGGCTGCTGGAACAGGAAGGCTTCAAGGTACGCATCGAGGACCTTATTGACCATCCTACC
ATGCTGGGACAGATGGCGGCGATGTGTTCTTCCAAAGTGGAGCAAAGCAAGATGACCTTT
ATGGATGAAGAATCTTCAATTCCCGCGACCCCCACCACGCCTAAAAGTGAAAAGAAGTCC
TGGCTGAAGGCCATGAACATGGCCCGCAAGATCGTAAAGCGGAACACACGGAGCTCT
Protein sequence predicted based on nucleotide sequence
SEYVITVNVETAKNDEKVVLRYWSDAIPDFEAKKYASSMAEILTKFLDQPRQKVATRDPA IKPSLEISPSEDQERSDSPAKQESERFYEQSVAGSSADLTKVVDSCVREVIQQMLENGTL KSNPSVNMADLVDRKISEILGQNQALIKPVPGSSHGTFSPYRKKAGLDLPSTSSDSYEVL DYNPKDDPDTHSMAAQIRRRGRSAVIEKKLLSLWSSMLEMDEDAIAPDDNFFELGGDSLT AMKLVGAAREDGLALTVADVFRNPVFEDMVTMIRVASLMTAYEDGDGDGDGDANSNGDLS SYHRTVPVLRSAAKSELYQRFSLVKATNIDAFLQSNICPKVGVFKGGIADVLPVTDFQGL AITGSLLESKWMLNYFFFDGNGPLDLKQLKLSAFRLVNSIDVLRTVFLPHGDRFLQVVLR KMRPDFFVYETESNMDEFTAMLQQRDRDQGPRLGEPFFNFIVVKRKDSDHHRIIIRISHA QYDGVCLPKIFLALQSAYQDEPLPPMSSFANYVRSTASTITSDHYQHWRALLKGSRMTEI VRRHGPNYRRSAGATTQLKQTILLPPIAHGSITTATVVKSAWALVLAQLTGRSDVVFGHT ISGRNATVPGVESTVGPCLNIVPVRVRFCEGWTALDLLRFVQDQQVSNMPYEALGFREIS KHCTEWPDWTNYTTVVQHQNVSYGSEMELGGNTYKIGGVGADEDFSDFSVISTPKGSDRC EISLSFSQNGDITPIFAQKVLNMLCNIASNFTAEPNSALMSPTELGELPPQVIDETGRQN DNYFVSSQLQGLSRADLLVLSDVLSRSWRQVLGDDNTNGLNLESSFFDLNGDIMGLAQVS WLLEQEGFKVRIEDLIDHPTMLGQMAAMCSSKVEQSKMTFMDEESSIPATPTTPKSEKKS WLKAMNMARKIVKRNTRSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
Pichia
Marker:
50ug/mL of Kanamycin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
