Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized by
Species:
Streptomyces ambofaciens
Type:
cDNA
Vector Name:
pET28b(+)
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Lawrence P. Wackett
Carrie Wilmot
University of Minnesota
Joint Genome Institute
Lawrence Berkeley National Laboratory
Carrie Wilmot
University of Minnesota
Joint Genome Institute
Lawrence Berkeley National Laboratory
SaCD00971605
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AVAILABLE
No restriction
Special MTA: LBNL
Special MTA: LBNL
Insert sequence: 1029nts
Open reading frame: 1 to 1029
ATGTCAGAAGCGATACGCGCACGCATTGCCGCAGTATGCGCCCATCTTCCAAGTCGGTAT
CGTACCCTGGATGAGACTCGGGCAAAGATTACAGAGACGGGAGGTTATGCTCCGCCGCCC
GGACTGTTAGAGGATCTTACAGGGGTGCGTGGAGTCCATGTTCATGAAGATGGTGAGAAT
TCAAGTGATCTGGCGGTGGCAGCCGCTAGGAGCGCACTGGAGCAGGCAGGACTTAAAATT
CGAGATGTTGACTTACTGTTATTCGCGTCGACTTCACAGGATTTGATCGAGCCGGCTACC
GCCCACCTTGTTGCAACCAAACTTGGAGCCAGCTGTCCAGTGTTTGACGTCAAAAATGCA
TGTAACAGCTTTCTGAATGGTATGGAAGTGGCAGCGTCCTTCATTGAGACGGGGCGGTAT
CGGACGGTCCTTATTGCTTGTGGGGAACCCGCTACCCTAGCGACTAGATGGCATTTGCCC
ACACGCGAAGATTTACTGAGAGCATTGCCTGGATATACCGCTTCTGATGCCGGTGCTGCA
ATGGTGCTCACAGCTGGCCCGGCGGGTGATGGCGACCCCGGAATTCTTAGCCTCCGCTTT
GTCTCAAACAGTCCGGCCTGGGAAGCGTGTACGGTTGCAGGGGGTGGCTCTATGCATCAT
TTGAGCGTTCATGATGACCATACGACCCTGCGACTAAATGGTGACCTCTGGCAGTCAGCG
CTCGATGTGTTACCGAGAATCATGGATACAGCAGGTGAGGAGTTTCGTGCAGCCCAGGCT
GGCGCAGTGTCCTTCATCGCCTTTCACCAGATTGCAATGCCGCAATATCGCGAAGTTATT
GATCTTTTCTCCTTACCTGAGGATCGCTGTATGCCAGCAGTGGCGGAGCACGGTAACTGC
GCGGCCGCGTCTCTGCCGCTGCAATTAGCCAAAGCCCGCGAGGCGGATCGTATTGAGGGG
GGTGATCTGGTCGCCATGATAGGCTTGGCATCAGGGTTCTCATTCGGACTTGCCTTGGTA
CGCTGGTGA
Protein sequence predicted based on nucleotide sequence
MSEAIRARIAAVCAHLPSRYRTLDETRAKITETGGYAPPPGLLEDLTGVRGVHVHEDGEN SSDLAVAAARSALEQAGLKIRDVDLLLFASTSQDLIEPATAHLVATKLGASCPVFDVKNA CNSFLNGMEVAASFIETGRYRTVLIACGEPATLATRWHLPTREDLLRALPGYTASDAGAA MVLTAGPAGDGDPGILSLRFVSNSPAWEACTVAGGGSMHHLSVHDDHTTLRLNGDLWQSA LDVLPRIMDTAGEEFRAAQAGAVSFIAFHQIAMPQYREVIDLFSLPEDRCMPAVAEHGNC AAASLPLQLAKAREADRIEGGDLVAMIGLASGFSFGLALVRW*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET28b(+)
Synonyms:
pET, pET28, pET28b, pET28b+, pET28b-plus
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5368
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, ssDNA production, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
0
0
bacterial origin
ori
pBR322-type bacterial origin of replication
0
0
promoter
T7
T7 promoter
370
386
repressor protein gene
lacI
lacI coding sequence
140
1852
selectable marker
KanR
kanamycin resistance gene
3995
4807
ssDNA origin
f1 ori
f1 origin for ssDNA production
4903
5358
tag
His
N-terminal His tag (after NcoI site in MCS)
270
287
tag
T7 tag
T7 tag (at NdeI site in MCS)
207
239
tag
His
C-terminal His tag (after XhoI in MCS)
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
