Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized by Homo sapiens
Species:
Coccidioides immitis RS
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Partial protein;XP_001239562:844-1761
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
CiCD00971695
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2754nts
Open reading frame: 1 to 2754
CACGTGAAGCAGGGCATCGCGAACGGCTCTGTTGATCCCTCAGTAACACCTGCTAGAGGC
GAAAGCAAGCACGTGCAGTTCGTGAGGGAGTTTGCCCTGTCGGCGTCCGTGTTACAAGCT
AAGAGTGCGACTCTTACATCCATCGACGAGAGTGTGCAGAAAACCATCTTGGAACTGAAG
AGCTGCCCTTACGAAGCCGACGCCTTCAGACAGAGACTGGATCAGATTCAAATCGCCGTG
GATAAGTTGAATCTCGAGAACTACGCCAACCTGGGCTTCTGGGTGGCCAATCTCAACAAC
ATGGTGGAAAGCATCCTGCAGGACCGGCTGCGGAGGGCTATTAGACATTGGATCCGGTCT
TTCCAGGAGGCGAAACATGAACAGGCTCACAAAATCTCTCACGGAGAGCTTTCCGATAGC
GCCGAGGAAGCCCAGATCCAAAATATACAGTTTCCGAACCTACTGCACGAGATCTCTATG
AGAAACCAGGTTATGCACCTTGACCCTCCTCTGGAGTTCGCCAGAGCGAACTGGTTTTCG
CACTTCGACCAGTGGGTGGGCGTCCTGTGCAACCTGGAAAGGATCAAGTCTTCCAGATAC
AAGATGAGCATCCATGCTGAGAAGGCCCGGCTGAGTGAGACACACTTTAGCGCCCTCCCC
CAGTACTGCGCCGACGAGTTGACTGAAGTTTACTCTGTCGTGGAAGCCAGACTTCAAGAG
ATCAGTGAGTACATTGAAAAATGGCTGCAGTTCCAATCTCTGTGGGACCTGCAGAGCTCT
TATGTGTATGACGTGTTGGGAGACGATCTCTCTCAGTGGCTCCAGCTTCTTCAGGAGATC
AGACGGAGCCGGGCGACCTTCGATACCAGCGAAGTCCGTCGGAGCTTCGGCACCATTAGA
ATCGACTACGAGCAGGTGCAGACAAAGGTGAACGCCAAATACGACCAATGGCAGAGAGAA
ATCCTCTTAAACTTCGGCGGCAAGTTGGGCACAAGAATGAGAGAGGTGTACGCCGAGCTG
CAAGCCGCGCGACGAGACCTGGAGGGCCAGTCGCTGGAAGCTTCCTCGACCGCCCACGCA
GTCTCTTTCATCACGATTGTGCAACAATGCAAAAGAAAAGCGAGAGTTTGGGAGCCTGAA
GTGGACCTGTTCAGGCAGGGACAAGCTACGCTGGCGAGGCAAAGATACCAGTTCCCTAAT
GACTGGCTGCATGTCGAGCAGGTGGATGGGGAATGGTTAGCCTTAAACGACATCTTAGAG
CGGCGCTCCAAGACTGTGCAAGATCAGACCGACGCCTTGAGAGCCAAGATCACAGCCGAA
GACAAGGTGATAAACGACAAGATCGCCGAAATCATCTCCCAGTGGAACGAGGAAAAGCCT
GTGAGTGGCAGCACCCCTCCTGAAGAAGCGAGTAGAACTCTGAGCCACTTCCAGAGCAGA
CTGGAGAGCCTCCAGTCTGAGTTCGAGATGGTGAGCAAGGCAAAGGAAGCGCTAGACCTT
CCAAGCAGTCCCGAGACAGCTCTGGCCGCCATCCTCGAAGAGGTGCAAGATTTTATGGCA
GTTTGGGCTGCTCTGTCTACAATCTGGAAGTCATTAAATGAACTGCGGGACGTACTGTGG
AATAGCGTGCAGCCCAGAAAGCTGAGGCAGAGCCTGGATGGACTGATCAGAATGACCAAG
GAAATGCCTAGCAGAATGAGACAGTACGCAGCCTTCGAACACATCCAGAACATCCTGAGA
CAGTTACTGAAAGTTAACCCACTGCTGTCCGATATGAAGAGCGAGGCTGTGCGGGAACGT
CATTGGCAAAAAATTTTCAAGGCACTCAAACCCGGCAAGAGATTTAGTCAGGTGAGTCTT
GCGCTCGGGGATGTGTGGGACCTGAATCTGGCAGCCAGCGAGGCCGTCATCCGGGACATA
ATCACTCAAGCCCAGGGCGAGATGGCCCTCGAAGAGTTCCTGAAGATGGTCCGGGAGACC
TGGCAAAGTTACTCACTAGACCTGGTGAATTATCAGAACAAGTGCAGACTGATCAAGGGC
TTTGATGACCTGTTTGCAAAATGCAGCGAAAATTTGAACTCGCTCCAGGCCATGCGGCAC
AGCCCGTACTACAAAGAATTTGAGGAAGAAGCCACAAGCTGGGAAGACAAGCTGAACAGA
GTTCACGTGCTGTTCGACGTGTGGATTGACGTGCAGCGGCAGTGGGTGTACCTCGAGGGC
GTCTTTACTGGCAACGCCGACATCAAGCACCTCCTGCCCTTGGAAAGCGGCAGATTCCAG
AACATCAACTCCGAATTTTTCGCGGTCATGAAAAAGGTGTACAAGAGCCCTTTCGTGTTG
GATGTGCTGGCCATCAACGGCGTGCAGAAGTCCCTGGAGAGACTGGCCGAGCTGCTGAAC
AAGATCCAGAAGGCCTTGGGAGAATACCTCGAACGGGAGCGCGTCAGCTTCCCTCGGTTC
TACTTCGTGGGAGACGAGGACCTGCTGGAGATCATTGGGAACTCTAACGATACACTGAGG
GTGGCCAAACACTTCAAGAAAATGTTCGCGGGTCTGTCGGGACTGGTTATGGATGATGAT
GGCAATATCGTGGGGTTTACGAGCAAAGAGGGCGAAGAAGTAAGATTGAAGAAGGAAGTG
TCTCTGCTCAAGAGCCCCAGGATTAATGACTGGCTGGCCGCCCTTGAGAACAGCATGAAG
AGCACACTCGCCGAGTTGCTGGCCGAAGCTATCGAGCAATTTGAATCCATTTAC
Protein sequence predicted based on nucleotide sequence
HVKQGIANGSVDPSVTPARGESKHVQFVREFALSASVLQAKSATLTSIDESVQKTILELK SCPYEADAFRQRLDQIQIAVDKLNLENYANLGFWVANLNNMVESILQDRLRRAIRHWIRS FQEAKHEQAHKISHGELSDSAEEAQIQNIQFPNLLHEISMRNQVMHLDPPLEFARANWFS HFDQWVGVLCNLERIKSSRYKMSIHAEKARLSETHFSALPQYCADELTEVYSVVEARLQE ISEYIEKWLQFQSLWDLQSSYVYDVLGDDLSQWLQLLQEIRRSRATFDTSEVRRSFGTIR IDYEQVQTKVNAKYDQWQREILLNFGGKLGTRMREVYAELQAARRDLEGQSLEASSTAHA VSFITIVQQCKRKARVWEPEVDLFRQGQATLARQRYQFPNDWLHVEQVDGEWLALNDILE RRSKTVQDQTDALRAKITAEDKVINDKIAEIISQWNEEKPVSGSTPPEEASRTLSHFQSR LESLQSEFEMVSKAKEALDLPSSPETALAAILEEVQDFMAVWAALSTIWKSLNELRDVLW NSVQPRKLRQSLDGLIRMTKEMPSRMRQYAAFEHIQNILRQLLKVNPLLSDMKSEAVRER HWQKIFKALKPGKRFSQVSLALGDVWDLNLAASEAVIRDIITQAQGEMALEEFLKMVRET WQSYSLDLVNYQNKCRLIKGFDDLFAKCSENLNSLQAMRHSPYYKEFEEEATSWEDKLNR VHVLFDVWIDVQRQWVYLEGVFTGNADIKHLLPLESGRFQNINSEFFAVMKKVYKSPFVL DVLAINGVQKSLERLAELLNKIQKALGEYLERERVSFPRFYFVGDEDLLEIIGNSNDTLR VAKHFKKMFAGLSGLVMDDDGNIVGFTSKEGEEVRLKKEVSLLKSPRINDWLAALENSMK STLAELLAEAIEQFESIY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
Pichia
Marker:
50ug/mL of Kanamycin
Host Type: bacterial Marker: kanamycin
Host Type: bacterial Marker: kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
