Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
codon optimized for Gene expression
Species:
Human coronavirus OC43
Type:
cDNA
Vector Name:
pCAGGS
Format:
CLOSED
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
Partial protein;6NZK_A:339-622;Domain 2;HCoV-OC43-like_Spike_S1_RBD;Domain 3;Domain 4.(1-66:Tissue plasminogen activator signal sequence);(70-921:RBD);(967-1851:HaloTag);(1855-1920:3xFLAG);Partial protein;6NZK_A:339-622;Domain 2;HCoV-OC43-like_Spike_S1_RBD;Domain 3;Domain 4
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Vel Murugan
Vel Murugan
HcCD00983872
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 1929nts
Open reading frame: 1 to 1929
ATGGACGCTATGAAGAGGGGACTGTGCTGCGTGCTGCTGCTGTGCGGAGCCGTGTTCGTG
TCCCCAAGCAAGCCTAACCTGCCAAACTGTAATATCGAGGCCTGGCTGAATGATAAGAGC
GTGCCATCTCCCCTGAACTGGGAGCGGAAGACCTTCTCTAACTGCAACTTCAACATGTCC
AGCCTGATGTCTTTCATCCAGGCTGACTCCTTTACCTGCAACAATATCGATGCCGCTAAA
ATCTACGGCATGTGCTTCTCTTCCATCACAATCGACAAGTTTGCCATCCCTAATGGCAGG
AAGGTGGATCTGCAGCTGGGCAATCTGGGCTACCTGCAGTCCTTTAACTATCGGATCGAC
ACCACAGCTACCAGCTGCCAGCTGTACTATAATCTGCCCGCCGCTAACGTGTCCGTGTCC
AGGTTCAACCCTTCTACATGGAACAAGAGGTTCGGCTTTATCGAGGACTCCGTGTTTAAG
CCTAGACCAGCCGGCGTGCTGACCAACCACGATGTGGTGTATGCTCAGCATTGTTTCAAG
GCCCCAAAGAATTTTTGCCCCTGTAAGCTGAACGGCTCTTGCGTGGGCTCCGGACCTGGC
AAGAACAATGGCATCGGCACATGTCCAGCTGGCACAAATTATCTGACCTGCGACAACCTG
TGCACCCCCGATCCTATCACCTTCACAGGCACCTACAAGTGCCCACAGACAAAGAGCCTG
GTGGGAATCGGAGAGCACTGCAGCGGCCTGGCCGTGAAGTCTGACTACTGTGGCGGCAAT
TCTTGCACCTGTCGCCCCCAGGCTTTTCTGGGATGGTCCGCCGACAGCTGCCTGCAGGGC
GATAAGTGTAATATCTTCGCTAACTTTATCCTGCATGACGTGAACAGCGGCCTGACATGT
TCTACCGATCTGCAGAAGGCTGGAGGAGGAGGCTCCGGCGGAGGAGGCAGCGGCGGCGGC
GGCTCTGAGATCGGCACAGGCTTCCCCTTTGACCCTCACTATGTGGAGGTGCTGGGCGAG
AGGATGCATTACGTGGACGTGGGACCTCGGGATGGCACACCAGTGCTGTTCCTGCACGGC
AATCCCACCTCCTCCTACGTGTGGAGAAACATCATCCCACACGTGGCTCCAACCCATAGG
TGCATCGCTCCCGACCTGATCGGCATGGGCAAGTCCGACAAGCCCGATCTGGGCTACTTC
TTTGACGATCATGTGAGGTTCATGGACGCCTTTATCGAGGCTCTGGGCCTGGAGGAGGTG
GTGCTGGTCATCCACGATTGGGGCTCCGCCCTGGGCTTTCATTGGGCCAAGAGGAATCCC
GAGCGGGTGAAGGGCATCGCTTTCATGGAGTTTATCAGACCAATCCCCACATGGGACGAG
TGGCCTGAGTTCGCTCGCGAGACCTTCCAGGCCTTTAGGACCACAGACGTGGGCCGGAAG
CTGATCATCGATCAGAACGTGTTCATCGAGGGCACACTGCCTATGGGCGTGGTGAGGCCT
CTGACCGAGGTGGAGATGGACCACTATCGGGAGCCTTTCCTGAACCCAGTGGATAGAGAG
CCTCTGTGGCGCTTTCCCAATGAGCTGCCTATCGCCGGCGAGCCAGCTAACATCGTGGCC
CTGGTGGAGGAGTACATGGATTGGCTGCATCAGTCCCCTGTGCCAAAGCTGCTGTTCTGG
GGCACACCTGGCGTGCTGATCCCACCTGCTGAGGCTGCTAGACTGGCCAAGAGCCTGCCA
AACTGTAAGGCCGTGGACATCGGCCCCGGCCTGAATCTGCTGCAGGAGGACAACCCCGAC
CTGATCGGCTCTGAGATCGCTCGCTGGCTGTCCACCCTGGAGATCAGCGGAGCTGATTAC
AAGGACCACGATGGCGACTATAAGGATCATGACATCGATTACAAGGACGATGACGATAAG
TGATGATGA
Protein sequence predicted based on nucleotide sequence
MDAMKRGLCCVLLLCGAVFVSPSKPNLPNCNIEAWLNDKSVPSPLNWERKTFSNCNFNMS SLMSFIQADSFTCNNIDAAKIYGMCFSSITIDKFAIPNGRKVDLQLGNLGYLQSFNYRID TTATSCQLYYNLPAANVSVSRFNPSTWNKRFGFIEDSVFKPRPAGVLTNHDVVYAQHCFK APKNFCPCKLNGSCVGSGPGKNNGIGTCPAGTNYLTCDNLCTPDPITFTGTYKCPQTKSL VGIGEHCSGLAVKSDYCGGNSCTCRPQAFLGWSADSCLQGDKCNIFANFILHDVNSGLTC STDLQKAGGGGSGGGGSGGGGSEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHG NPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRK LIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPD LIGSEIARWLSTLEISGADYKDHDGDYKDHDIDYKDDDDK***
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pCAGGS
Synonyms:
''
Description:
multiple cloning site clone, mammalian expression expression with CMV and beta globin and M13 reverse and ColE and AmpR and chimeric intron and CAGF and MCS and CAGR and lac operator and lac and CAP binding site and SV40 and SV40 PolyA; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
4790
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site
Author Name:
Miyazaki, J
Publications:
PMID:
1660837
Title: Efficient selection for high-expression transfectants with a novel eukaryotic vector
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin of replication
3022
3704
gene fragment
beta globin
Rabbit beta globin gene fragment
1630
2258
intron
chimeric intron
Chimeric Intron Var 002
662
1670
primer site
M13 reverse
M13 reverse primer binding site
2289
2309
promoter
CMV
CMV immediate early promoter
4
384
selectable marker
AmpR
ampicillin resistance gene
3802
4461
