Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
codon optimized by Homo sapiens for Gene expression
Species:
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Type:
cDNA
Vector Name:
pAH_nHalo_3xFlag
Format:
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Vel Murugan
Vel Murugan
CjCD00984086
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 870nts
Open reading frame: 1 to 870
ATGGACGCCATGAAAAGAGGCCTGTGCTGTGTGCTGCTCCTGTGCGGCGCCGTTTTCGTG
TCCCCTATGCAGAAGATCATCGTGTTTATCCTGTGTTGCTTCATGACATTCTTCCTGTAC
GCCTGCAGCAGCAAGTTCGAGAACGTGAACCCTCTTGGAAGATCTTTCGGCGAGTTCGAA
GATACCGACCCCCTGAAGCTGGGACTGGAACCCACCTTTCCAACCAACCAGGAGATCCCC
AGCCTGATCAGCGGCGCCGACCTGGTGCCTATTACTCCAATCACACCTCCTCTGACCCGG
ACCAGCAACAGCGCCAACAACAACGCCGCTAATGGCATCAACCCCAGATTCAAGGATGAG
GCCTTTAACGATGTGCTGATTTTCGAGAACCGGCCTGCCGTCTCCGATTTTCTGACCATC
CTGGGACCTTCTGGCGCTGCTCTGACAGTGTGGGCCCTGGCTCAAGGCAATTGGATCTGG
GGCTACACCCTGATCGACAGCAAGGGCTTCGGCGATGCCAGAGTGTGGCAGCTGCTGCTG
TATCCTAACGACTTCGCCATGATCAAAAACGCCAAGACCAATACCTGTCTGAACGCCTAC
GGCAACGGCATCGTGCACTACCCCTGCGACGCTTCTAATCACGCCCAGATGTGGAAGCTG
ATCCCTATGAGCAACACCGCCGTGCAGATCAAGAACCTGGGAAATGGCAAGTGCATCCAG
GCCCCTATCACCAACCTGTACGGCGACTTCCACAAAGTGTTTAAGATCTTCACCGTGGAA
TGCGCCAAAAAGGACAACTTCGACCAACAGTGGTTCCTGACAACACCTCCATTCACAGCC
AAGCCCCTGTACAGACAGGGCGAGGTGCGG
Protein sequence predicted based on nucleotide sequence
MDAMKRGLCCVLLLCGAVFVSPMQKIIVFILCCFMTFFLYACSSKFENVNPLGRSFGEFE DTDPLKLGLEPTFPTNQEIPSLISGADLVPITPITPPLTRTSNSANNNAANGINPRFKDE AFNDVLIFENRPAVSDFLTILGPSGAALTVWALAQGNWIWGYTLIDSKGFGDARVWQLLL YPNDFAMIKNAKTNTCLNAYGNGIVHYPCDASNHAQMWKLIPMSNTAVQIKNLGNGKCIQ APITNLYGDFHKVFKIFTVECAKKDNFDQQWFLTTPPFTAKPLYRQGEVR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pAH_nHalo_3xFlag
Synonyms:
''
Description:
with tag/fusion/marker assay, restriction enzyme clone, mammalian expression expression with CMV enhancer and chicken_beta_actin_promoter-var002 and chimeric intron and HaloTag(R) and 3xFlag and beta globin and M13 reverse and lac operator and lac promoter and CAP binding site and ori and AmpR and AmpR promoter; ampicillin resistance in bacterial;
Comments:
None
Size (bp):
5541
Parent Vector:
None
Properties:
mammalian expression, restriction enzyme, with tag/fusion/marker
Author Name:
Vel Murugan
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
HaloTag(R)
modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
1816
2706
CDS
3xFlag
three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
2737
2802
CDS
AmpR
confers resistance to ampicillin, carbenicillin, and related antibiotics
4550
5410
enhancer
CMV enhancer
human cytomegalovirus immediate early enhancer
4
380
intron
chimeric intron
chimera between introns from chicken beta-actin and rabbit beta-globin
662
1674
poly-A signal
beta globin
rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)
2963
3018
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3377
3393
promoter
chicken_beta_actin_promoter-var002
NULL
386
661
promoter
lac promoter
promoter for the E. coli lac operon
3425
3455
promoter
AmpR promoter
NULL
5411
5515
protein binding site
lac operator
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
3401
3417
protein binding site
CAP binding site
CAP binding activates transcription in the presence of cAMP.
3470
3491
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3791
4379
