Vector Name:
pET28b(+)
Synonyms:
pET, pET28, pET28b, pET28b+, pET28b-plus
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5368
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, ssDNA production, with tag/fusion/marker
Author Name:
Novagen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
0
0
bacterial origin
ori
pBR322-type bacterial origin of replication
0
0
promoter
T7
T7 promoter
370
386
repressor protein gene
lacI
lacI coding sequence
140
1852
selectable marker
KanR
kanamycin resistance gene
3995
4807
ssDNA origin
f1 ori
f1 origin for ssDNA production
4903
5358
tag
His
N-terminal His tag (after NcoI site in MCS)
270
287
tag
T7 tag
T7 tag (at NdeI site in MCS)
207
239
tag
His
C-terminal His tag (after XhoI in MCS)
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
