Vector Name:
pSB2
Synonyms:
pSB-2, pSB2_TN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
TOPO modified pSB2 vector -by Invitrogen. Stop codon generated by adding an extra G to the reverse primer which puts the insert in-frame with vector-encoded stop when cloned into the TA site.
Size (bp):
5338
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5110
5119
bacterial origin
ori
ColE1 bacterial origin of replication
1420
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3770
4594
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5079
5096
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5267
5313
