ID pDNR-Dual standard; circular DNA; ; 4938 BP. CC This file is created by Vector NTI CC http://www.informaxinc.com/ CC VNTDATE|373826522| CC VNTDBDATE|373831246| CC LSOWNER| CC VNTAUTHORNAME|Stephanie Mohr| CC VNTAUTHORTEL|617.324.4251| CC VNTAUTHOREML|stephanie_mohr@hms.harvard.edu| FH Key Location/Qualifiers FH FT CDS 1509..2927 FT /vntifkey="4" FT /label=sacB FT /note="sacB death cassette gene from B. subtilis" FT CDS 3103..3960 FT /vntifkey="4" FT /label=beta-lactamase\(ampR) FT CDS complement(330..1031) FT /vntifkey="4" FT /label=chlR\ORF FT misc_feature 1015..1048 FT /vntifkey="21" FT /label=LoxP FT misc_feature 9..42 FT /vntifkey="21" FT /label=LoxP FT misc_feature 67..95 FT /vntifkey="21" FT /label=MCS\(gene\inserted\here) FT /note="MCS at HIP use reaction to clone into this region" SQ Sequence 4938 BP; 1492 A; 1122 C; 1091 G; 1233 t; gcggccgcat aacttcgtat agcatacatt atacgaagtt atcagtcgac ggtaccggac 60 atatgcccgg gaattcctgc aggatccgct cgagaagctt tctagaccat tcgtttggcg 120 cgcgggccca ggtgagtggt cataatcata atcataatca taatcataat cacaactagc 180 ctaggagatc ctggtcatga ctagtgcttg gattctcacc aataaaaaac gcccggcggc 240 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca 300 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt 360 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga 420 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg 480 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag 540 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt 600 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg 660 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg 720 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga 780 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc 840 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga 900 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg 960 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaag atccataact 1020 tcgtatagca tacattatac gaagttatgc ggccgcgacg tccacatata cctgccgttc 1080 actattattt agtgaaatga gatattatga tattttctga attgtgatta aaaaggcaac 1140 tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa cagatagatt 1200 ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc aaacaaaaga 1260 ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg aaaagtaaat 1320 cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta 1380 tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta actaacttgc 1440 catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata aaaaaggaga 1500 catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg 1560 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata 1620 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc 1680 aaaaaaatga aaaatatcaa gttcctgagt tcgattcgtc cacaattaaa aatatctctt 1740 ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg 1800 caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg 1860 acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa 1920 acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag 1980 accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat 2040 tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta 2100 acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct 2160 ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca 2220 gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat 2280 acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat 2340 ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc 2400 tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc 2460 taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa 2520 cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg 2580 actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg 2640 gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt 2700 taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc 2760 aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag 2820 acaaacaatc aacgtttgcg cctagcttcc tgctgaacat caaaggcaag aaaacatctg 2880 ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa aaacgcaaaa 2940 gaaaatgccg atatcctatt ggcattgacg tcaggtggca cttttcgggg aaatgtgcgc 3000 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 3060 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 3120 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 3180 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 3240 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 3300 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 3360 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 3420 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 3480 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 3540 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 3600 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 3660 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 3720 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 3780 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 3840 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 3900 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 3960 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 4020 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4080 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4140 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4200 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4260 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 4320 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4380 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4440 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4500 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 4560 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4620 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 4680 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 4740 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 4800 cctgattctg tggataaccg tattaccgcc ttacgcgtgt aaaacgacgg ccagtagatc 4860 tgtaatacga ctcactatag ggcgctagct gctcgccgca gccgaacgac cgagcgcagc 4920 gagtcagtga gcgaggaa 4938 //